Experiment: DinoMM minimal media with L-Arginine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c21220 and PGA1_c21230 are separated by 67 nucleotides PGA1_c21230 and PGA1_c21240 overlap by 4 nucleotides PGA1_c21240 and PGA1_c21250 are separated by 109 nucleotides
PGA1_c21220: PGA1_c21220 - transcriptional regulator, MarR family, at 2,197,275 to 2,197,778
_c21220
PGA1_c21230: PGA1_c21230 - Uncharacterized conserved protein, at 2,197,846 to 2,198,106
_c21230
PGA1_c21240: PGA1_c21240 - helix-turn-helix domain-containing protein, at 2,198,103 to 2,198,498
_c21240
PGA1_c21250: PGA1_c21250 - aspartate aminotransferase AatA, at 2,198,608 to 2,199,810
_c21250
Position (kb)
2197
2198
2199 Strain fitness (log2 ratio)
-2
-1
0
1 at 2196.849 kb on + strand at 2196.857 kb on - strand at 2196.857 kb on - strand at 2196.862 kb on + strand at 2196.864 kb on + strand at 2196.870 kb on - strand at 2196.993 kb on + strand at 2197.001 kb on - strand at 2197.083 kb on + strand at 2197.147 kb on + strand at 2197.295 kb on + strand at 2197.338 kb on - strand, within PGA1_c21220 at 2197.520 kb on + strand, within PGA1_c21220 at 2197.709 kb on + strand, within PGA1_c21220 at 2197.881 kb on - strand, within PGA1_c21230 at 2198.521 kb on + strand at 2198.541 kb on - strand at 2198.561 kb on - strand at 2198.566 kb on + strand at 2198.566 kb on + strand at 2198.566 kb on + strand at 2198.566 kb on + strand at 2198.566 kb on + strand at 2198.566 kb on + strand at 2198.566 kb on + strand at 2198.566 kb on + strand at 2198.574 kb on - strand at 2198.574 kb on - strand at 2198.574 kb on - strand at 2198.574 kb on - strand at 2198.574 kb on - strand at 2198.644 kb on - strand at 2198.886 kb on + strand, within PGA1_c21250 at 2199.074 kb on + strand, within PGA1_c21250
Per-strain Table
Position Strand Gene LocusTag Fraction DinoMM minimal media with L-Arginine remove 2,196,849 + +0.2 2,196,857 - -0.5 2,196,857 - +1.2 2,196,862 + -2.1 2,196,864 + -1.1 2,196,870 - -0.9 2,196,993 + -0.8 2,197,001 - +1.1 2,197,083 + +0.8 2,197,147 + -0.9 2,197,295 + -1.4 2,197,338 - PGA1_c21220 0.12 -1.3 2,197,520 + PGA1_c21220 0.49 -0.9 2,197,709 + PGA1_c21220 0.86 -1.1 2,197,881 - PGA1_c21230 0.13 +0.8 2,198,521 + -0.3 2,198,541 - -1.1 2,198,561 - -1.2 2,198,566 + -1.1 2,198,566 + -0.5 2,198,566 + +0.1 2,198,566 + -0.8 2,198,566 + -0.8 2,198,566 + +0.1 2,198,566 + -0.9 2,198,566 + -0.5 2,198,574 - -0.2 2,198,574 - -2.2 2,198,574 - -0.9 2,198,574 - -2.5 2,198,574 - -1.1 2,198,644 - -0.9 2,198,886 + PGA1_c21250 0.23 +0.1 2,199,074 + PGA1_c21250 0.39 -1.4
Or see this region's nucleotide sequence