Experiment: DinoMM minimal media with L-Arginine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c21160 and PGA1_c21170 are separated by 72 nucleotides PGA1_c21170 and PGA1_c21180 are separated by 12 nucleotides PGA1_c21180 and PGA1_c21190 are separated by 300 nucleotides
PGA1_c21160: PGA1_c21160 - putative metallopeptidase, at 2,190,966 to 2,192,123
_c21160
PGA1_c21170: PGA1_c21170 - dihydrofolate reductase FolA, at 2,192,196 to 2,192,672
_c21170
PGA1_c21180: PGA1_c21180 - thymidylate synthase ThyA, at 2,192,685 to 2,193,518
_c21180
PGA1_c21190: PGA1_c21190 - hypothetical protein, at 2,193,819 to 2,194,757
_c21190
Position (kb)
2192
2193
2194 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2191.699 kb on + strand, within PGA1_c21160 at 2191.715 kb on + strand, within PGA1_c21160 at 2191.812 kb on - strand, within PGA1_c21160 at 2191.921 kb on + strand, within PGA1_c21160 at 2191.927 kb on + strand, within PGA1_c21160 at 2191.935 kb on - strand, within PGA1_c21160 at 2191.954 kb on - strand, within PGA1_c21160 at 2191.954 kb on - strand, within PGA1_c21160 at 2191.995 kb on - strand, within PGA1_c21160 at 2192.023 kb on - strand at 2192.133 kb on + strand at 2192.672 kb on + strand at 2192.680 kb on - strand at 2192.680 kb on - strand at 2193.614 kb on - strand at 2193.668 kb on - strand at 2193.668 kb on - strand at 2193.668 kb on - strand at 2193.687 kb on - strand at 2193.774 kb on + strand at 2193.840 kb on - strand at 2193.872 kb on - strand at 2193.945 kb on + strand, within PGA1_c21190 at 2194.082 kb on + strand, within PGA1_c21190 at 2194.090 kb on - strand, within PGA1_c21190 at 2194.090 kb on - strand at 2194.090 kb on - strand, within PGA1_c21190 at 2194.090 kb on - strand, within PGA1_c21190 at 2194.091 kb on - strand, within PGA1_c21190 at 2194.092 kb on + strand, within PGA1_c21190 at 2194.092 kb on + strand, within PGA1_c21190 at 2194.092 kb on + strand, within PGA1_c21190 at 2194.092 kb on + strand, within PGA1_c21190 at 2194.092 kb on + strand, within PGA1_c21190 at 2194.092 kb on + strand, within PGA1_c21190 at 2194.095 kb on - strand, within PGA1_c21190 at 2194.205 kb on + strand, within PGA1_c21190 at 2194.288 kb on + strand, within PGA1_c21190 at 2194.293 kb on + strand, within PGA1_c21190 at 2194.368 kb on + strand, within PGA1_c21190 at 2194.390 kb on - strand, within PGA1_c21190 at 2194.490 kb on - strand, within PGA1_c21190
Per-strain Table
Position Strand Gene LocusTag Fraction DinoMM minimal media with L-Arginine remove 2,191,699 + PGA1_c21160 0.63 +1.1 2,191,715 + PGA1_c21160 0.65 -0.2 2,191,812 - PGA1_c21160 0.73 +0.7 2,191,921 + PGA1_c21160 0.82 +1.1 2,191,927 + PGA1_c21160 0.83 +0.7 2,191,935 - PGA1_c21160 0.84 +0.7 2,191,954 - PGA1_c21160 0.85 -0.8 2,191,954 - PGA1_c21160 0.85 +1.2 2,191,995 - PGA1_c21160 0.89 -1.1 2,192,023 - -0.9 2,192,133 + +0.2 2,192,672 + +0.4 2,192,680 - -1.9 2,192,680 - +0.8 2,193,614 - -0.7 2,193,668 - +0.6 2,193,668 - -0.9 2,193,668 - +1.4 2,193,687 - -0.6 2,193,774 + +0.3 2,193,840 - +1.2 2,193,872 - -1.4 2,193,945 + PGA1_c21190 0.13 -1.4 2,194,082 + PGA1_c21190 0.28 +0.8 2,194,090 - PGA1_c21190 0.29 -0.4 2,194,090 - -0.6 2,194,090 - PGA1_c21190 0.29 -0.6 2,194,090 - PGA1_c21190 0.29 +0.1 2,194,091 - PGA1_c21190 0.29 -0.6 2,194,092 + PGA1_c21190 0.29 +0.2 2,194,092 + PGA1_c21190 0.29 -0.2 2,194,092 + PGA1_c21190 0.29 -1.3 2,194,092 + PGA1_c21190 0.29 +0.3 2,194,092 + PGA1_c21190 0.29 +1.4 2,194,092 + PGA1_c21190 0.29 +1.1 2,194,095 - PGA1_c21190 0.29 -0.4 2,194,205 + PGA1_c21190 0.41 -0.3 2,194,288 + PGA1_c21190 0.50 +0.7 2,194,293 + PGA1_c21190 0.50 +0.6 2,194,368 + PGA1_c21190 0.58 -1.2 2,194,390 - PGA1_c21190 0.61 +2.0 2,194,490 - PGA1_c21190 0.71 -0.7
Or see this region's nucleotide sequence