Experiment: DinoMM minimal media with L-Arginine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c17670 and PGA1_c17680 are separated by 126 nucleotides PGA1_c17680 and PGA1_c17690 are separated by 450 nucleotides PGA1_c17690 and PGA1_c17700 are separated by 292 nucleotides PGA1_c17700 and PGA1_c17710 are separated by 12 nucleotides
PGA1_c17670: PGA1_c17670 - transcriptional regulator, crp family, at 1,850,475 to 1,851,233
_c17670
PGA1_c17680: PGA1_c17680 - hypothetical protein, at 1,851,360 to 1,851,596
_c17680
PGA1_c17690: PGA1_c17690 - serine acetyltransferase CysE, at 1,852,047 to 1,852,862
_c17690
PGA1_c17700: PGA1_c17700 - hypothetical protein, at 1,853,155 to 1,853,451
_c17700
PGA1_c17710: PGA1_c17710 - putative gene transfer agent protein, at 1,853,464 to 1,857,432
_c17710
Position (kb)
1852
1853 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1851.053 kb on - strand, within PGA1_c17670 at 1851.134 kb on + strand, within PGA1_c17670 at 1851.134 kb on - strand, within PGA1_c17670 at 1851.134 kb on - strand, within PGA1_c17670 at 1851.222 kb on - strand at 1851.222 kb on - strand at 1851.223 kb on - strand at 1851.249 kb on + strand at 1851.249 kb on + strand at 1851.249 kb on + strand at 1851.249 kb on + strand at 1851.249 kb on + strand at 1851.249 kb on + strand at 1851.249 kb on + strand at 1851.249 kb on + strand at 1851.252 kb on - strand at 1851.252 kb on - strand at 1851.254 kb on + strand at 1851.257 kb on - strand at 1851.257 kb on - strand at 1851.257 kb on - strand at 1851.257 kb on - strand at 1851.257 kb on - strand at 1851.259 kb on + strand at 1851.270 kb on + strand at 1851.270 kb on + strand at 1851.270 kb on + strand at 1851.270 kb on + strand at 1851.278 kb on - strand at 1851.311 kb on - strand at 1851.388 kb on - strand, within PGA1_c17680 at 1851.560 kb on + strand, within PGA1_c17680 at 1851.676 kb on + strand at 1851.681 kb on + strand at 1851.682 kb on - strand at 1851.836 kb on + strand at 1851.859 kb on - strand at 1851.911 kb on + strand at 1851.954 kb on + strand at 1852.020 kb on - strand at 1853.127 kb on + strand at 1853.150 kb on - strand at 1853.215 kb on - strand, within PGA1_c17700 at 1853.215 kb on - strand, within PGA1_c17700 at 1853.215 kb on - strand, within PGA1_c17700 at 1853.249 kb on - strand, within PGA1_c17700 at 1853.300 kb on + strand, within PGA1_c17700 at 1853.313 kb on - strand, within PGA1_c17700 at 1853.436 kb on + strand at 1853.480 kb on - strand at 1853.530 kb on - strand at 1853.541 kb on + strand at 1853.601 kb on - strand at 1853.601 kb on - strand at 1853.624 kb on - strand at 1853.650 kb on + strand at 1853.775 kb on + strand at 1853.777 kb on + strand at 1853.783 kb on - strand at 1853.785 kb on + strand at 1853.817 kb on + strand at 1853.835 kb on - strand at 1853.835 kb on - strand at 1853.855 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction DinoMM minimal media with L-Arginine remove 1,851,053 - PGA1_c17670 0.76 -1.1 1,851,134 + PGA1_c17670 0.87 -0.0 1,851,134 - PGA1_c17670 0.87 +0.2 1,851,134 - PGA1_c17670 0.87 +0.7 1,851,222 - +0.4 1,851,222 - +0.7 1,851,223 - +1.4 1,851,249 + -2.6 1,851,249 + -1.0 1,851,249 + +1.2 1,851,249 + -0.8 1,851,249 + +0.6 1,851,249 + -0.3 1,851,249 + +1.7 1,851,249 + +0.3 1,851,252 - -0.2 1,851,252 - -1.5 1,851,254 + +1.1 1,851,257 - -0.3 1,851,257 - +0.6 1,851,257 - -0.6 1,851,257 - +0.1 1,851,257 - -0.1 1,851,259 + +1.2 1,851,270 + +0.7 1,851,270 + -0.2 1,851,270 + -1.8 1,851,270 + -1.3 1,851,278 - +0.5 1,851,311 - -0.4 1,851,388 - PGA1_c17680 0.12 -0.3 1,851,560 + PGA1_c17680 0.84 +0.0 1,851,676 + +0.7 1,851,681 + +0.4 1,851,682 - -0.8 1,851,836 + -0.9 1,851,859 - +0.8 1,851,911 + +0.2 1,851,954 + -1.9 1,852,020 - -0.7 1,853,127 + +0.7 1,853,150 - +0.5 1,853,215 - PGA1_c17700 0.20 -1.0 1,853,215 - PGA1_c17700 0.20 +2.0 1,853,215 - PGA1_c17700 0.20 +1.2 1,853,249 - PGA1_c17700 0.32 -0.9 1,853,300 + PGA1_c17700 0.49 +0.4 1,853,313 - PGA1_c17700 0.53 +0.3 1,853,436 + -0.0 1,853,480 - +0.6 1,853,530 - +0.2 1,853,541 + +0.1 1,853,601 - -0.1 1,853,601 - +0.3 1,853,624 - +0.9 1,853,650 + +0.1 1,853,775 + +0.0 1,853,777 + +0.5 1,853,783 - -0.9 1,853,785 + -0.4 1,853,817 + -0.6 1,853,835 - -0.9 1,853,835 - -0.4 1,853,855 + -0.1
Or see this region's nucleotide sequence