Experiment: DinoMM minimal media with L-Arginine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c12600 and PGA1_c12610 are separated by 9 nucleotides PGA1_c12610 and PGA1_c12620 are separated by 159 nucleotides PGA1_c12620 and PGA1_c12630 are separated by 196 nucleotides
PGA1_c12600: PGA1_c12600 - biotin carboxylase AccA, at 1,305,561 to 1,306,913
_c12600
PGA1_c12610: PGA1_c12610 - biotin carboxyl carrier protein of acetyl-CoA carboxylase AccB, at 1,306,923 to 1,307,420
_c12610
PGA1_c12620: PGA1_c12620 - putative transcriptional regulator, araC family, at 1,307,580 to 1,308,116
_c12620
PGA1_c12630: PGA1_c12630 - putative high-affinity branched-chain amino acid transport ATP-binding protein, at 1,308,313 to 1,309,068
_c12630
Position (kb)
1306
1307
1308 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1307.479 kb on + strand at 1307.540 kb on + strand at 1307.576 kb on - strand at 1307.581 kb on - strand at 1307.581 kb on - strand at 1307.581 kb on - strand at 1307.581 kb on - strand at 1307.669 kb on - strand, within PGA1_c12620 at 1307.725 kb on - strand, within PGA1_c12620 at 1307.756 kb on + strand, within PGA1_c12620 at 1307.756 kb on + strand, within PGA1_c12620 at 1307.756 kb on + strand, within PGA1_c12620 at 1307.756 kb on + strand, within PGA1_c12620 at 1307.756 kb on + strand, within PGA1_c12620 at 1307.760 kb on - strand, within PGA1_c12620 at 1307.764 kb on - strand, within PGA1_c12620 at 1307.764 kb on - strand, within PGA1_c12620 at 1307.764 kb on - strand, within PGA1_c12620 at 1307.764 kb on - strand, within PGA1_c12620 at 1307.799 kb on + strand, within PGA1_c12620 at 1307.884 kb on + strand, within PGA1_c12620 at 1307.971 kb on + strand, within PGA1_c12620 at 1307.971 kb on + strand, within PGA1_c12620 at 1307.979 kb on - strand, within PGA1_c12620 at 1307.979 kb on - strand, within PGA1_c12620 at 1307.979 kb on - strand, within PGA1_c12620 at 1307.979 kb on - strand, within PGA1_c12620 at 1307.979 kb on - strand, within PGA1_c12620 at 1307.988 kb on + strand, within PGA1_c12620 at 1308.024 kb on - strand, within PGA1_c12620 at 1308.124 kb on + strand at 1308.129 kb on + strand at 1308.134 kb on + strand at 1308.177 kb on + strand at 1308.196 kb on + strand at 1308.214 kb on - strand at 1308.260 kb on + strand at 1308.268 kb on - strand at 1308.296 kb on + strand at 1308.306 kb on + strand at 1308.332 kb on - strand at 1308.345 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction DinoMM minimal media with L-Arginine remove 1,307,479 + +1.5 1,307,540 + -1.1 1,307,576 - +0.6 1,307,581 - +0.4 1,307,581 - +1.1 1,307,581 - -0.5 1,307,581 - +2.0 1,307,669 - PGA1_c12620 0.17 +0.1 1,307,725 - PGA1_c12620 0.27 -0.9 1,307,756 + PGA1_c12620 0.33 -2.4 1,307,756 + PGA1_c12620 0.33 -0.6 1,307,756 + PGA1_c12620 0.33 -0.8 1,307,756 + PGA1_c12620 0.33 -0.2 1,307,756 + PGA1_c12620 0.33 +1.1 1,307,760 - PGA1_c12620 0.34 -0.7 1,307,764 - PGA1_c12620 0.34 -0.5 1,307,764 - PGA1_c12620 0.34 -2.1 1,307,764 - PGA1_c12620 0.34 -0.1 1,307,764 - PGA1_c12620 0.34 +0.4 1,307,799 + PGA1_c12620 0.41 +1.0 1,307,884 + PGA1_c12620 0.57 +1.0 1,307,971 + PGA1_c12620 0.73 +0.6 1,307,971 + PGA1_c12620 0.73 -0.8 1,307,979 - PGA1_c12620 0.74 -0.0 1,307,979 - PGA1_c12620 0.74 -0.9 1,307,979 - PGA1_c12620 0.74 +0.9 1,307,979 - PGA1_c12620 0.74 -1.2 1,307,979 - PGA1_c12620 0.74 +0.8 1,307,988 + PGA1_c12620 0.76 +0.3 1,308,024 - PGA1_c12620 0.83 +0.9 1,308,124 + +1.1 1,308,129 + -0.6 1,308,134 + +0.2 1,308,177 + +0.5 1,308,196 + -1.8 1,308,214 - -0.1 1,308,260 + +0.7 1,308,268 - +1.1 1,308,296 + +0.1 1,308,306 + +0.9 1,308,332 - +0.3 1,308,345 - -0.2
Or see this region's nucleotide sequence