Experiment: DinoMM minimal media with L-Arginine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c12580 and PGA1_c12590 overlap by 37 nucleotides PGA1_c12590 and PGA1_c12600 are separated by 118 nucleotides PGA1_c12600 and PGA1_c12610 are separated by 9 nucleotides PGA1_c12610 and PGA1_c12620 are separated by 159 nucleotides
PGA1_c12580: PGA1_c12580 - Uncharacterized protein conserved in bacteria (DUF2155)., at 1,304,484 to 1,304,846
_c12580
PGA1_c12590: PGA1_c12590 - leucyl/phenylalanyl-tRNA--protein transferase Aat, at 1,304,810 to 1,305,442
_c12590
PGA1_c12600: PGA1_c12600 - biotin carboxylase AccA, at 1,305,561 to 1,306,913
_c12600
PGA1_c12610: PGA1_c12610 - biotin carboxyl carrier protein of acetyl-CoA carboxylase AccB, at 1,306,923 to 1,307,420
_c12610
PGA1_c12620: PGA1_c12620 - putative transcriptional regulator, araC family, at 1,307,580 to 1,308,116
_c12620
Position (kb)
1305
1306
1307 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1304.591 kb on + strand, within PGA1_c12580 at 1304.591 kb on + strand, within PGA1_c12580 at 1304.591 kb on + strand, within PGA1_c12580 at 1304.599 kb on - strand, within PGA1_c12580 at 1304.706 kb on + strand, within PGA1_c12580 at 1304.713 kb on + strand, within PGA1_c12580 at 1304.773 kb on + strand, within PGA1_c12580 at 1304.773 kb on + strand, within PGA1_c12580 at 1304.773 kb on + strand, within PGA1_c12580 at 1304.821 kb on + strand at 1304.938 kb on + strand, within PGA1_c12590 at 1304.938 kb on + strand, within PGA1_c12590 at 1304.938 kb on + strand, within PGA1_c12590 at 1304.946 kb on - strand, within PGA1_c12590 at 1304.946 kb on - strand, within PGA1_c12590 at 1304.946 kb on - strand, within PGA1_c12590 at 1304.983 kb on + strand, within PGA1_c12590 at 1305.040 kb on + strand, within PGA1_c12590 at 1305.083 kb on + strand, within PGA1_c12590 at 1305.083 kb on + strand, within PGA1_c12590 at 1305.123 kb on - strand, within PGA1_c12590 at 1305.285 kb on + strand, within PGA1_c12590 at 1305.285 kb on - strand, within PGA1_c12590 at 1305.293 kb on - strand, within PGA1_c12590 at 1305.293 kb on - strand, within PGA1_c12590 at 1305.293 kb on - strand, within PGA1_c12590 at 1305.293 kb on - strand, within PGA1_c12590 at 1305.293 kb on - strand, within PGA1_c12590 at 1305.293 kb on - strand, within PGA1_c12590 at 1305.338 kb on - strand, within PGA1_c12590 at 1305.359 kb on - strand, within PGA1_c12590 at 1305.369 kb on - strand, within PGA1_c12590 at 1305.369 kb on - strand, within PGA1_c12590 at 1305.369 kb on - strand, within PGA1_c12590 at 1305.424 kb on + strand at 1305.424 kb on + strand at 1305.424 kb on + strand at 1305.424 kb on + strand at 1305.432 kb on - strand at 1305.432 kb on - strand at 1305.445 kb on - strand at 1305.505 kb on + strand at 1305.513 kb on - strand at 1305.568 kb on - strand at 1307.479 kb on + strand at 1307.540 kb on + strand at 1307.576 kb on - strand at 1307.581 kb on - strand at 1307.581 kb on - strand at 1307.581 kb on - strand at 1307.581 kb on - strand at 1307.669 kb on - strand, within PGA1_c12620 at 1307.725 kb on - strand, within PGA1_c12620 at 1307.756 kb on + strand, within PGA1_c12620 at 1307.756 kb on + strand, within PGA1_c12620 at 1307.756 kb on + strand, within PGA1_c12620 at 1307.756 kb on + strand, within PGA1_c12620 at 1307.756 kb on + strand, within PGA1_c12620 at 1307.760 kb on - strand, within PGA1_c12620 at 1307.764 kb on - strand, within PGA1_c12620 at 1307.764 kb on - strand, within PGA1_c12620 at 1307.764 kb on - strand, within PGA1_c12620 at 1307.764 kb on - strand, within PGA1_c12620 at 1307.799 kb on + strand, within PGA1_c12620 at 1307.884 kb on + strand, within PGA1_c12620
Per-strain Table
Position Strand Gene LocusTag Fraction DinoMM minimal media with L-Arginine remove 1,304,591 + PGA1_c12580 0.29 +0.2 1,304,591 + PGA1_c12580 0.29 -0.7 1,304,591 + PGA1_c12580 0.29 +1.4 1,304,599 - PGA1_c12580 0.32 -0.2 1,304,706 + PGA1_c12580 0.61 -0.1 1,304,713 + PGA1_c12580 0.63 -1.8 1,304,773 + PGA1_c12580 0.80 -0.4 1,304,773 + PGA1_c12580 0.80 -1.7 1,304,773 + PGA1_c12580 0.80 +0.4 1,304,821 + +0.9 1,304,938 + PGA1_c12590 0.20 +1.4 1,304,938 + PGA1_c12590 0.20 -0.1 1,304,938 + PGA1_c12590 0.20 +1.5 1,304,946 - PGA1_c12590 0.21 -1.1 1,304,946 - PGA1_c12590 0.21 -1.4 1,304,946 - PGA1_c12590 0.21 -0.2 1,304,983 + PGA1_c12590 0.27 -0.6 1,305,040 + PGA1_c12590 0.36 -2.4 1,305,083 + PGA1_c12590 0.43 +0.2 1,305,083 + PGA1_c12590 0.43 -0.6 1,305,123 - PGA1_c12590 0.49 -0.5 1,305,285 + PGA1_c12590 0.75 +0.5 1,305,285 - PGA1_c12590 0.75 +0.7 1,305,293 - PGA1_c12590 0.76 +0.5 1,305,293 - PGA1_c12590 0.76 -1.4 1,305,293 - PGA1_c12590 0.76 -0.4 1,305,293 - PGA1_c12590 0.76 -0.2 1,305,293 - PGA1_c12590 0.76 +1.0 1,305,293 - PGA1_c12590 0.76 -1.5 1,305,338 - PGA1_c12590 0.83 -0.8 1,305,359 - PGA1_c12590 0.87 +0.1 1,305,369 - PGA1_c12590 0.88 -0.1 1,305,369 - PGA1_c12590 0.88 -0.6 1,305,369 - PGA1_c12590 0.88 -0.2 1,305,424 + +1.2 1,305,424 + +0.5 1,305,424 + -0.4 1,305,424 + -0.9 1,305,432 - -0.6 1,305,432 - +2.2 1,305,445 - +0.6 1,305,505 + -0.4 1,305,513 - +0.6 1,305,568 - -0.8 1,307,479 + +1.5 1,307,540 + -1.1 1,307,576 - +0.6 1,307,581 - +0.4 1,307,581 - +1.1 1,307,581 - -0.5 1,307,581 - +2.0 1,307,669 - PGA1_c12620 0.17 +0.1 1,307,725 - PGA1_c12620 0.27 -0.9 1,307,756 + PGA1_c12620 0.33 -2.4 1,307,756 + PGA1_c12620 0.33 -0.6 1,307,756 + PGA1_c12620 0.33 -0.8 1,307,756 + PGA1_c12620 0.33 -0.2 1,307,756 + PGA1_c12620 0.33 +1.1 1,307,760 - PGA1_c12620 0.34 -0.7 1,307,764 - PGA1_c12620 0.34 -0.5 1,307,764 - PGA1_c12620 0.34 -2.1 1,307,764 - PGA1_c12620 0.34 -0.1 1,307,764 - PGA1_c12620 0.34 +0.4 1,307,799 + PGA1_c12620 0.41 +1.0 1,307,884 + PGA1_c12620 0.57 +1.0
Or see this region's nucleotide sequence