Experiment: DinoMM minimal media with L-Arginine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c11250 and PGA1_c11255 are separated by 60 nucleotides PGA1_c11255 and PGA1_c11260 are separated by 219 nucleotides PGA1_c11260 and PGA1_c11270 are separated by 87 nucleotides PGA1_c11270 and PGA1_c11280 are separated by 211 nucleotides
PGA1_c11250: PGA1_c11250 - fatty acid/phospholipid synthesis protein PlsX, at 1,163,471 to 1,164,574
_c11250
PGA1_c11255: PGA1_c11255 - 50S ribosomal protein L32, at 1,164,635 to 1,164,841
_c11255
PGA1_c11260: PGA1_c11260 - Predicted metal-binding, possibly nucleic acid-binding protein, at 1,165,061 to 1,165,606
_c11260
PGA1_c11270: PGA1_c11270 - Small protein A (tmRNA-binding), at 1,165,694 to 1,166,161
_c11270
PGA1_c11280: PGA1_c11280 - Putative hemolysin, at 1,166,373 to 1,167,236
_c11280
Position (kb)
1165
1166 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1164.590 kb on - strand at 1165.695 kb on - strand at 1166.174 kb on + strand at 1166.182 kb on - strand at 1166.192 kb on + strand at 1166.192 kb on + strand at 1166.200 kb on - strand at 1166.244 kb on - strand at 1166.249 kb on - strand at 1166.333 kb on + strand at 1166.354 kb on + strand at 1166.354 kb on + strand at 1166.359 kb on + strand at 1166.364 kb on + strand at 1166.364 kb on + strand at 1166.367 kb on - strand at 1166.372 kb on - strand at 1166.429 kb on - strand at 1166.436 kb on - strand at 1166.436 kb on - strand at 1166.437 kb on + strand at 1166.444 kb on + strand at 1166.552 kb on + strand, within PGA1_c11280 at 1166.575 kb on + strand, within PGA1_c11280 at 1166.575 kb on + strand, within PGA1_c11280 at 1166.575 kb on + strand, within PGA1_c11280 at 1166.575 kb on + strand, within PGA1_c11280
Per-strain Table
Position Strand Gene LocusTag Fraction DinoMM minimal media with L-Arginine remove 1,164,590 - +0.0 1,165,695 - +0.4 1,166,174 + -0.5 1,166,182 - -0.6 1,166,192 + +1.3 1,166,192 + -0.2 1,166,200 - +0.5 1,166,244 - -1.2 1,166,249 - +0.6 1,166,333 + -0.6 1,166,354 + -0.2 1,166,354 + -1.6 1,166,359 + +3.0 1,166,364 + +0.0 1,166,364 + +0.3 1,166,367 - -1.5 1,166,372 - +0.9 1,166,429 - +0.6 1,166,436 - +0.6 1,166,436 - -1.9 1,166,437 + +0.1 1,166,444 + -0.3 1,166,552 + PGA1_c11280 0.21 +2.0 1,166,575 + PGA1_c11280 0.23 -0.1 1,166,575 + PGA1_c11280 0.23 -0.8 1,166,575 + PGA1_c11280 0.23 +0.4 1,166,575 + PGA1_c11280 0.23 -0.6
Or see this region's nucleotide sequence