Experiment: Varel_Bryant_medium_Glucose with Ceftriaxone hemi(heptahydrate) 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2680 and BT2681 are separated by 388 nucleotides BT2681 and BT2682 overlap by 14 nucleotides BT2682 and BT2683 are separated by 28 nucleotides
BT2680: BT2680 - beta-galactosidase (NCBI ptt file), at 3,338,137 to 3,341,142
BT2680
BT2681: BT2681 - alginate O-acetylation protein (NCBI ptt file), at 3,341,531 to 3,343,018
BT2681
BT2682: BT2682 - putative periplasmic protein (NCBI ptt file), at 3,343,005 to 3,343,871
BT2682
BT2683: BT2683 - putative periplasmic protein (NCBI ptt file), at 3,343,900 to 3,345,294
BT2683
Position (kb)
3341
3342
3343
3344 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3340.555 kb on - strand, within BT2680 at 3340.557 kb on - strand, within BT2680 at 3340.557 kb on - strand, within BT2680 at 3340.557 kb on - strand, within BT2680 at 3340.558 kb on + strand, within BT2680 at 3340.683 kb on - strand, within BT2680 at 3340.737 kb on + strand, within BT2680 at 3340.738 kb on - strand, within BT2680 at 3340.738 kb on - strand at 3340.738 kb on - strand, within BT2680 at 3340.804 kb on + strand, within BT2680 at 3340.807 kb on - strand, within BT2680 at 3340.844 kb on - strand at 3340.902 kb on + strand at 3340.902 kb on + strand at 3340.903 kb on - strand at 3341.033 kb on + strand at 3341.059 kb on - strand at 3341.064 kb on + strand at 3341.064 kb on + strand at 3341.068 kb on - strand at 3341.099 kb on - strand at 3341.099 kb on - strand at 3341.133 kb on + strand at 3341.143 kb on - strand at 3341.143 kb on - strand at 3341.145 kb on + strand at 3341.145 kb on + strand at 3341.145 kb on + strand at 3341.146 kb on - strand at 3341.152 kb on - strand at 3341.217 kb on - strand at 3341.220 kb on + strand at 3341.221 kb on - strand at 3341.261 kb on - strand at 3341.263 kb on + strand at 3341.297 kb on - strand at 3341.405 kb on - strand at 3341.405 kb on - strand at 3341.405 kb on - strand at 3341.505 kb on + strand at 3341.545 kb on - strand at 3341.621 kb on + strand at 3341.638 kb on + strand at 3341.771 kb on + strand, within BT2681 at 3341.772 kb on - strand, within BT2681 at 3341.854 kb on + strand, within BT2681 at 3341.929 kb on + strand, within BT2681 at 3341.930 kb on - strand, within BT2681 at 3341.930 kb on - strand, within BT2681 at 3341.930 kb on - strand, within BT2681 at 3342.071 kb on + strand, within BT2681 at 3342.072 kb on - strand, within BT2681 at 3342.075 kb on + strand, within BT2681 at 3342.075 kb on + strand, within BT2681 at 3342.109 kb on + strand, within BT2681 at 3342.110 kb on - strand, within BT2681 at 3342.219 kb on + strand, within BT2681 at 3342.252 kb on + strand, within BT2681 at 3342.253 kb on - strand, within BT2681 at 3342.336 kb on + strand, within BT2681 at 3342.377 kb on - strand, within BT2681 at 3342.440 kb on - strand, within BT2681 at 3342.495 kb on + strand, within BT2681 at 3342.495 kb on + strand, within BT2681 at 3342.495 kb on + strand, within BT2681 at 3342.496 kb on - strand, within BT2681 at 3342.513 kb on - strand, within BT2681 at 3342.589 kb on + strand, within BT2681 at 3342.593 kb on + strand, within BT2681 at 3342.593 kb on + strand, within BT2681 at 3342.593 kb on + strand, within BT2681 at 3342.594 kb on - strand, within BT2681 at 3342.606 kb on - strand, within BT2681 at 3342.608 kb on - strand, within BT2681 at 3342.679 kb on - strand, within BT2681 at 3342.679 kb on - strand, within BT2681 at 3342.810 kb on + strand, within BT2681 at 3342.826 kb on + strand, within BT2681 at 3342.827 kb on - strand, within BT2681 at 3342.829 kb on - strand, within BT2681 at 3342.831 kb on + strand, within BT2681 at 3342.837 kb on + strand, within BT2681 at 3342.865 kb on + strand, within BT2681 at 3342.874 kb on + strand at 3342.876 kb on + strand at 3342.877 kb on - strand at 3342.877 kb on - strand at 3342.877 kb on - strand at 3342.881 kb on - strand at 3342.897 kb on + strand at 3342.934 kb on + strand at 3342.973 kb on + strand at 3342.973 kb on + strand at 3342.973 kb on + strand at 3342.974 kb on - strand at 3342.974 kb on - strand at 3343.018 kb on + strand at 3343.027 kb on + strand at 3343.027 kb on + strand at 3343.051 kb on - strand at 3343.070 kb on + strand at 3343.073 kb on - strand at 3343.073 kb on - strand at 3343.158 kb on + strand, within BT2682 at 3343.159 kb on - strand, within BT2682 at 3343.166 kb on - strand, within BT2682 at 3343.367 kb on + strand, within BT2682 at 3343.369 kb on + strand, within BT2682 at 3343.512 kb on - strand, within BT2682 at 3343.517 kb on + strand, within BT2682 at 3343.518 kb on - strand, within BT2682 at 3343.519 kb on + strand, within BT2682 at 3343.611 kb on - strand, within BT2682 at 3343.642 kb on - strand, within BT2682 at 3343.692 kb on + strand, within BT2682 at 3343.692 kb on + strand, within BT2682 at 3343.751 kb on + strand, within BT2682 at 3343.884 kb on - strand at 3343.902 kb on - strand at 3343.914 kb on - strand at 3343.931 kb on + strand at 3343.932 kb on - strand at 3343.947 kb on - strand at 3343.948 kb on + strand at 3343.949 kb on - strand at 3343.949 kb on - strand at 3343.961 kb on + strand at 3344.007 kb on + strand at 3344.007 kb on + strand at 3344.008 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Ceftriaxone hemi(heptahydrate) 0.1 mM remove 3,340,555 - BT2680 0.80 -2.1 3,340,557 - BT2680 0.81 +1.6 3,340,557 - BT2680 0.81 -1.0 3,340,557 - BT2680 0.81 -0.0 3,340,558 + BT2680 0.81 +0.3 3,340,683 - BT2680 0.85 -1.1 3,340,737 + BT2680 0.86 -0.3 3,340,738 - BT2680 0.87 +0.5 3,340,738 - -0.6 3,340,738 - BT2680 0.87 -0.9 3,340,804 + BT2680 0.89 +0.5 3,340,807 - BT2680 0.89 -1.4 3,340,844 - -0.4 3,340,902 + -2.0 3,340,902 + -0.3 3,340,903 - -3.1 3,341,033 + -0.0 3,341,059 - -0.6 3,341,064 + +0.6 3,341,064 + -0.8 3,341,068 - -0.9 3,341,099 - -0.9 3,341,099 - +1.5 3,341,133 + +2.0 3,341,143 - -0.6 3,341,143 - +2.3 3,341,145 + -0.7 3,341,145 + +1.0 3,341,145 + -0.4 3,341,146 - +2.0 3,341,152 - -1.8 3,341,217 - -0.3 3,341,220 + -2.3 3,341,221 - -2.8 3,341,261 - -0.0 3,341,263 + -0.8 3,341,297 - -1.4 3,341,405 - -0.5 3,341,405 - +1.0 3,341,405 - -0.0 3,341,505 + -1.2 3,341,545 - +0.4 3,341,621 + -0.0 3,341,638 + -0.0 3,341,771 + BT2681 0.16 -2.0 3,341,772 - BT2681 0.16 -1.4 3,341,854 + BT2681 0.22 -2.0 3,341,929 + BT2681 0.27 -1.1 3,341,930 - BT2681 0.27 -1.8 3,341,930 - BT2681 0.27 -0.2 3,341,930 - BT2681 0.27 -1.3 3,342,071 + BT2681 0.36 -0.6 3,342,072 - BT2681 0.36 -1.7 3,342,075 + BT2681 0.37 -2.3 3,342,075 + BT2681 0.37 -1.4 3,342,109 + BT2681 0.39 -1.8 3,342,110 - BT2681 0.39 +1.0 3,342,219 + BT2681 0.46 -1.6 3,342,252 + BT2681 0.48 +1.0 3,342,253 - BT2681 0.49 -1.3 3,342,336 + BT2681 0.54 +2.0 3,342,377 - BT2681 0.57 -2.0 3,342,440 - BT2681 0.61 -1.3 3,342,495 + BT2681 0.65 -1.9 3,342,495 + BT2681 0.65 +0.5 3,342,495 + BT2681 0.65 -0.0 3,342,496 - BT2681 0.65 -1.6 3,342,513 - BT2681 0.66 -1.3 3,342,589 + BT2681 0.71 +1.0 3,342,593 + BT2681 0.71 -1.5 3,342,593 + BT2681 0.71 -1.4 3,342,593 + BT2681 0.71 -3.0 3,342,594 - BT2681 0.71 +0.4 3,342,606 - BT2681 0.72 -2.0 3,342,608 - BT2681 0.72 -2.2 3,342,679 - BT2681 0.77 -0.0 3,342,679 - BT2681 0.77 -1.3 3,342,810 + BT2681 0.86 -0.6 3,342,826 + BT2681 0.87 -1.6 3,342,827 - BT2681 0.87 -1.1 3,342,829 - BT2681 0.87 +1.0 3,342,831 + BT2681 0.87 -1.2 3,342,837 + BT2681 0.88 -1.2 3,342,865 + BT2681 0.90 -1.6 3,342,874 + -1.3 3,342,876 + -1.6 3,342,877 - +1.0 3,342,877 - -2.0 3,342,877 - -2.6 3,342,881 - +0.3 3,342,897 + -0.0 3,342,934 + -1.6 3,342,973 + +0.8 3,342,973 + -2.6 3,342,973 + -1.0 3,342,974 - +0.6 3,342,974 - +2.0 3,343,018 + -0.0 3,343,027 + -1.1 3,343,027 + -0.4 3,343,051 - -0.0 3,343,070 + -0.9 3,343,073 - -1.6 3,343,073 - -0.3 3,343,158 + BT2682 0.18 -0.4 3,343,159 - BT2682 0.18 -0.5 3,343,166 - BT2682 0.19 -0.9 3,343,367 + BT2682 0.42 -1.2 3,343,369 + BT2682 0.42 -0.0 3,343,512 - BT2682 0.58 -1.1 3,343,517 + BT2682 0.59 -0.6 3,343,518 - BT2682 0.59 +0.2 3,343,519 + BT2682 0.59 -1.3 3,343,611 - BT2682 0.70 -1.4 3,343,642 - BT2682 0.73 -1.0 3,343,692 + BT2682 0.79 -0.0 3,343,692 + BT2682 0.79 -1.1 3,343,751 + BT2682 0.86 -1.5 3,343,884 - -1.2 3,343,902 - -0.3 3,343,914 - -0.6 3,343,931 + -0.6 3,343,932 - -1.9 3,343,947 - -2.0 3,343,948 + -0.0 3,343,949 - -0.5 3,343,949 - -1.0 3,343,961 + -0.0 3,344,007 + -0.9 3,344,007 + -0.0 3,344,008 - -1.0
Or see this region's nucleotide sequence