Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS26825

Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS26810 and BWI76_RS26815 are separated by 83 nucleotidesBWI76_RS26815 and BWI76_RS26820 are separated by 351 nucleotidesBWI76_RS26820 and BWI76_RS26825 overlap by 20 nucleotidesBWI76_RS26825 and BWI76_RS26830 are separated by 48 nucleotides BWI76_RS26810: BWI76_RS26810 - cellulose biosynthesis protein BcsG, at 5,558,766 to 5,560,442 _RS26810 BWI76_RS26815: BWI76_RS26815 - small toxic polypeptide ldrD (Modular protein), at 5,560,526 to 5,560,651 _RS26815 BWI76_RS26820: BWI76_RS26820 - small toxic polypeptide ldrD (Modular protein), at 5,561,003 to 5,561,128 _RS26820 BWI76_RS26825: BWI76_RS26825 - hypothetical protein, at 5,561,109 to 5,561,327 _RS26825 BWI76_RS26830: BWI76_RS26830 - endoglucanase, at 5,561,376 to 5,562,380 _RS26830 Position (kb) 5561 5562Strain fitness (log2 ratio) -2 -1 0 1at 5560.369 kb on - strandat 5560.399 kb on + strandat 5560.492 kb on - strandat 5560.729 kb on - strandat 5560.964 kb on - strandat 5561.144 kb on + strand, within BWI76_RS26825at 5561.342 kb on + strandat 5561.398 kb on + strandat 5561.477 kb on + strand, within BWI76_RS26830at 5561.477 kb on + strand, within BWI76_RS26830at 5561.544 kb on - strand, within BWI76_RS26830at 5561.772 kb on + strand, within BWI76_RS26830at 5561.829 kb on + strand, within BWI76_RS26830at 5561.860 kb on + strand, within BWI76_RS26830at 5561.868 kb on - strand, within BWI76_RS26830at 5561.876 kb on - strand, within BWI76_RS26830at 5562.152 kb on + strand, within BWI76_RS26830at 5562.160 kb on - strand, within BWI76_RS26830at 5562.324 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM
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5,560,369 - +1.0
5,560,399 + -0.2
5,560,492 - -1.1
5,560,729 - +0.4
5,560,964 - +1.4
5,561,144 + BWI76_RS26825 0.16 -0.3
5,561,342 + +0.4
5,561,398 + -1.2
5,561,477 + BWI76_RS26830 0.10 +0.6
5,561,477 + BWI76_RS26830 0.10 +1.7
5,561,544 - BWI76_RS26830 0.17 +0.5
5,561,772 + BWI76_RS26830 0.39 -2.1
5,561,829 + BWI76_RS26830 0.45 -0.3
5,561,860 + BWI76_RS26830 0.48 -0.5
5,561,868 - BWI76_RS26830 0.49 +0.3
5,561,876 - BWI76_RS26830 0.50 -0.1
5,562,152 + BWI76_RS26830 0.77 +0.9
5,562,160 - BWI76_RS26830 0.78 -0.5
5,562,324 - +0.3

Or see this region's nucleotide sequence