Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS25685

Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS25680 and BWI76_RS25685 are separated by 21 nucleotidesBWI76_RS25685 and BWI76_RS25690 are separated by 140 nucleotidesBWI76_RS25690 and BWI76_RS25695 overlap by 47 nucleotidesBWI76_RS25695 and BWI76_RS25700 are separated by 58 nucleotides BWI76_RS25680: BWI76_RS25680 - 16S rRNA m5C967 methyltransferase, at 5,331,836 to 5,333,125 _RS25680 BWI76_RS25685: BWI76_RS25685 - Trk system potassium transport protein TrkA, at 5,333,147 to 5,334,523 _RS25685 BWI76_RS25690: BWI76_RS25690 - large-conductance mechanosensitive channel, at 5,334,664 to 5,335,077 _RS25690 BWI76_RS25695: BWI76_RS25695 - ribosome alternative rescue factor ArfA, at 5,335,031 to 5,335,291 _RS25695 BWI76_RS25700: BWI76_RS25700 - heavy metal-responsive transcriptional regulator, at 5,335,350 to 5,335,775 _RS25700 Position (kb) 5333 5334 5335Strain fitness (log2 ratio) -2 -1 0 1 2at 5332.349 kb on + strand, within BWI76_RS25680at 5332.352 kb on + strand, within BWI76_RS25680at 5332.481 kb on - strand, within BWI76_RS25680at 5332.546 kb on - strand, within BWI76_RS25680at 5332.651 kb on + strand, within BWI76_RS25680at 5332.882 kb on + strand, within BWI76_RS25680at 5332.911 kb on + strand, within BWI76_RS25680at 5332.911 kb on + strand, within BWI76_RS25680at 5333.008 kb on + strandat 5333.048 kb on + strandat 5333.055 kb on - strandat 5333.098 kb on + strandat 5334.496 kb on + strandat 5334.515 kb on + strandat 5334.538 kb on - strandat 5334.545 kb on + strandat 5334.638 kb on + strandat 5334.638 kb on + strandat 5334.638 kb on + strandat 5334.638 kb on + strandat 5334.638 kb on + strandat 5334.646 kb on - strandat 5334.646 kb on - strandat 5334.646 kb on - strandat 5334.647 kb on + strandat 5334.730 kb on + strand, within BWI76_RS25690at 5334.745 kb on + strand, within BWI76_RS25690at 5334.745 kb on + strand, within BWI76_RS25690at 5334.748 kb on - strand, within BWI76_RS25690at 5334.793 kb on + strand, within BWI76_RS25690at 5334.799 kb on + strand, within BWI76_RS25690at 5334.801 kb on - strand, within BWI76_RS25690at 5334.829 kb on - strand, within BWI76_RS25690at 5334.851 kb on - strand, within BWI76_RS25690at 5334.852 kb on + strand, within BWI76_RS25690at 5334.854 kb on + strand, within BWI76_RS25690at 5334.874 kb on + strand, within BWI76_RS25690at 5334.874 kb on + strand, within BWI76_RS25690at 5334.874 kb on - strand, within BWI76_RS25690at 5334.882 kb on + strand, within BWI76_RS25690at 5334.997 kb on + strand, within BWI76_RS25690at 5335.000 kb on + strand, within BWI76_RS25690at 5335.001 kb on + strand, within BWI76_RS25690at 5335.006 kb on + strand, within BWI76_RS25690at 5335.019 kb on + strand, within BWI76_RS25690at 5335.032 kb on - strandat 5335.032 kb on - strandat 5335.032 kb on - strandat 5335.127 kb on + strand, within BWI76_RS25695at 5335.136 kb on - strand, within BWI76_RS25695at 5335.143 kb on - strand, within BWI76_RS25695at 5335.189 kb on - strand, within BWI76_RS25695at 5335.189 kb on - strand, within BWI76_RS25695at 5335.209 kb on - strand, within BWI76_RS25695at 5335.226 kb on + strand, within BWI76_RS25695at 5335.248 kb on - strand, within BWI76_RS25695at 5335.269 kb on + strandat 5335.277 kb on - strandat 5335.287 kb on + strandat 5335.305 kb on - strandat 5335.326 kb on + strandat 5335.326 kb on + strandat 5335.326 kb on + strandat 5335.326 kb on + strandat 5335.334 kb on - strandat 5335.416 kb on + strand, within BWI76_RS25700at 5335.424 kb on - strand, within BWI76_RS25700at 5335.424 kb on - strand, within BWI76_RS25700at 5335.424 kb on - strand, within BWI76_RS25700at 5335.512 kb on + strand, within BWI76_RS25700at 5335.520 kb on - strand, within BWI76_RS25700

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM
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5,332,349 + BWI76_RS25680 0.40 -1.1
5,332,352 + BWI76_RS25680 0.40 +0.2
5,332,481 - BWI76_RS25680 0.50 -0.3
5,332,546 - BWI76_RS25680 0.55 -0.1
5,332,651 + BWI76_RS25680 0.63 -0.2
5,332,882 + BWI76_RS25680 0.81 +0.6
5,332,911 + BWI76_RS25680 0.83 -0.1
5,332,911 + BWI76_RS25680 0.83 -0.9
5,333,008 + -2.1
5,333,048 + -1.9
5,333,055 - +0.2
5,333,098 + +0.3
5,334,496 + +0.3
5,334,515 + +1.2
5,334,538 - -2.0
5,334,545 + -0.8
5,334,638 + +0.8
5,334,638 + -0.1
5,334,638 + +2.3
5,334,638 + +0.7
5,334,638 + -0.3
5,334,646 - +0.7
5,334,646 - -0.4
5,334,646 - +1.0
5,334,647 + +0.4
5,334,730 + BWI76_RS25690 0.16 +0.2
5,334,745 + BWI76_RS25690 0.20 +0.2
5,334,745 + BWI76_RS25690 0.20 +0.2
5,334,748 - BWI76_RS25690 0.20 -0.9
5,334,793 + BWI76_RS25690 0.31 +1.2
5,334,799 + BWI76_RS25690 0.33 +0.2
5,334,801 - BWI76_RS25690 0.33 -0.1
5,334,829 - BWI76_RS25690 0.40 +1.2
5,334,851 - BWI76_RS25690 0.45 -0.1
5,334,852 + BWI76_RS25690 0.45 -0.1
5,334,854 + BWI76_RS25690 0.46 +2.0
5,334,874 + BWI76_RS25690 0.51 -0.0
5,334,874 + BWI76_RS25690 0.51 +0.4
5,334,874 - BWI76_RS25690 0.51 -0.2
5,334,882 + BWI76_RS25690 0.53 -0.8
5,334,997 + BWI76_RS25690 0.80 +0.2
5,335,000 + BWI76_RS25690 0.81 -0.2
5,335,001 + BWI76_RS25690 0.81 +0.3
5,335,006 + BWI76_RS25690 0.83 +0.4
5,335,019 + BWI76_RS25690 0.86 +0.5
5,335,032 - +0.8
5,335,032 - +1.1
5,335,032 - -0.1
5,335,127 + BWI76_RS25695 0.37 -0.9
5,335,136 - BWI76_RS25695 0.40 -1.1
5,335,143 - BWI76_RS25695 0.43 +0.8
5,335,189 - BWI76_RS25695 0.61 -0.1
5,335,189 - BWI76_RS25695 0.61 +1.2
5,335,209 - BWI76_RS25695 0.68 -0.1
5,335,226 + BWI76_RS25695 0.75 +0.6
5,335,248 - BWI76_RS25695 0.83 +0.9
5,335,269 + +1.1
5,335,277 - +0.2
5,335,287 + +2.5
5,335,305 - +1.2
5,335,326 + +0.8
5,335,326 + -1.0
5,335,326 + +0.5
5,335,326 + +0.1
5,335,334 - -0.1
5,335,416 + BWI76_RS25700 0.15 -0.2
5,335,424 - BWI76_RS25700 0.17 +0.2
5,335,424 - BWI76_RS25700 0.17 +0.1
5,335,424 - BWI76_RS25700 0.17 +0.8
5,335,512 + BWI76_RS25700 0.38 +1.1
5,335,520 - BWI76_RS25700 0.40 +0.3

Or see this region's nucleotide sequence