Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS25625

Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS25620 and BWI76_RS25625 are separated by 53 nucleotidesBWI76_RS25625 and BWI76_RS25630 are separated by 21 nucleotidesBWI76_RS25630 and BWI76_RS25635 overlap by 2 nucleotidesBWI76_RS25635 and BWI76_RS25640 overlap by 25 nucleotidesBWI76_RS25640 and BWI76_RS25645 overlap by 4 nucleotides BWI76_RS25620: BWI76_RS25620 - 16S ribosomal RNA, at 5,323,839 to 5,325,392 _RS25620 BWI76_RS25625: BWI76_RS25625 - hypothetical protein, at 5,325,446 to 5,325,651 _RS25625 BWI76_RS25630: BWI76_RS25630 - hypothetical protein, at 5,325,673 to 5,325,861 _RS25630 BWI76_RS25635: BWI76_RS25635 - gamma carbonic anhydrase family protein, at 5,325,860 to 5,326,414 _RS25635 BWI76_RS25640: BWI76_RS25640 - hypothetical protein, at 5,326,390 to 5,326,647 _RS25640 BWI76_RS25645: BWI76_RS25645 - shikimate dehydrogenase, at 5,326,644 to 5,327,462 _RS25645 Position (kb) 5325 5326Strain fitness (log2 ratio) -2 -1 0 1at 5325.798 kb on - strand, within BWI76_RS25630at 5325.807 kb on - strand, within BWI76_RS25630at 5326.136 kb on - strand, within BWI76_RS25635at 5326.136 kb on - strand, within BWI76_RS25635at 5326.264 kb on + strandat 5326.278 kb on + strand, within BWI76_RS25635at 5326.287 kb on - strand, within BWI76_RS25635at 5326.342 kb on + strand, within BWI76_RS25635at 5326.350 kb on - strand, within BWI76_RS25635at 5326.403 kb on + strandat 5326.403 kb on + strandat 5326.406 kb on - strandat 5326.426 kb on - strand, within BWI76_RS25640at 5326.426 kb on - strand, within BWI76_RS25640at 5326.491 kb on + strand, within BWI76_RS25640at 5326.533 kb on + strand, within BWI76_RS25640at 5326.548 kb on - strand, within BWI76_RS25640at 5326.565 kb on - strand, within BWI76_RS25640

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM
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5,325,798 - BWI76_RS25630 0.66 -0.5
5,325,807 - BWI76_RS25630 0.71 +0.5
5,326,136 - BWI76_RS25635 0.50 +0.5
5,326,136 - BWI76_RS25635 0.50 +0.2
5,326,264 + +0.2
5,326,278 + BWI76_RS25635 0.75 -0.2
5,326,287 - BWI76_RS25635 0.77 -0.3
5,326,342 + BWI76_RS25635 0.87 -2.1
5,326,350 - BWI76_RS25635 0.88 -0.7
5,326,403 + +0.5
5,326,403 + +0.1
5,326,406 - +1.2
5,326,426 - BWI76_RS25640 0.14 -0.7
5,326,426 - BWI76_RS25640 0.14 +0.8
5,326,491 + BWI76_RS25640 0.39 +0.0
5,326,533 + BWI76_RS25640 0.55 -1.9
5,326,548 - BWI76_RS25640 0.61 -0.4
5,326,565 - BWI76_RS25640 0.68 -1.1

Or see this region's nucleotide sequence