Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS25350

Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS25335 and BWI76_RS25340 overlap by 2 nucleotidesBWI76_RS25340 and BWI76_RS25345 are separated by 109 nucleotidesBWI76_RS25345 and BWI76_RS25350 are separated by 15 nucleotidesBWI76_RS25350 and BWI76_RS25355 are separated by 219 nucleotides BWI76_RS25335: BWI76_RS25335 - stringent starvation protein A, at 5,268,028 to 5,268,666 _RS25335 BWI76_RS25340: BWI76_RS25340 - hypothetical protein, at 5,268,665 to 5,268,868 _RS25340 BWI76_RS25345: BWI76_RS25345 - 30S ribosomal protein S9, at 5,268,978 to 5,269,370 _RS25345 BWI76_RS25350: BWI76_RS25350 - 50S ribosomal protein L13, at 5,269,386 to 5,269,814 _RS25350 BWI76_RS25355: BWI76_RS25355 - cell division protein ZapE, at 5,270,034 to 5,271,167 _RS25355 Position (kb) 5269 5270Strain fitness (log2 ratio) -1 0 1 2at 5269.977 kb on + strandat 5270.031 kb on + strandat 5270.186 kb on + strand, within BWI76_RS25355at 5270.294 kb on + strand, within BWI76_RS25355at 5270.490 kb on + strand, within BWI76_RS25355at 5270.654 kb on + strand, within BWI76_RS25355at 5270.654 kb on + strand, within BWI76_RS25355at 5270.807 kb on - strand, within BWI76_RS25355

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM
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5,269,977 + +1.2
5,270,031 + +1.2
5,270,186 + BWI76_RS25355 0.13 +0.2
5,270,294 + BWI76_RS25355 0.23 +1.1
5,270,490 + BWI76_RS25355 0.40 +2.7
5,270,654 + BWI76_RS25355 0.55 -0.7
5,270,654 + BWI76_RS25355 0.55 +0.3
5,270,807 - BWI76_RS25355 0.68 -0.3

Or see this region's nucleotide sequence