Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS25110

Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS25095 and BWI76_RS25100 are separated by 27 nucleotidesBWI76_RS25100 and BWI76_RS25105 are separated by 206 nucleotidesBWI76_RS25105 and BWI76_RS25110 are separated by 130 nucleotidesBWI76_RS25110 and BWI76_RS25115 overlap by 21 nucleotides BWI76_RS25095: BWI76_RS25095 - transcription termination protein NusA, at 5,225,395 to 5,226,882 _RS25095 BWI76_RS25100: BWI76_RS25100 - ribosome maturation factor, at 5,226,910 to 5,227,362 _RS25100 BWI76_RS25105: BWI76_RS25105 - tRNA-Met, at 5,227,569 to 5,227,645 _RS25105 BWI76_RS25110: BWI76_RS25110 - hypothetical protein, at 5,227,776 to 5,227,973 _RS25110 BWI76_RS25115: BWI76_RS25115 - argininosuccinate synthase, at 5,227,953 to 5,229,296 _RS25115 Position (kb) 5227 5228Strain fitness (log2 ratio) -3 -2 -1 0 1at 5228.036 kb on + strandat 5228.036 kb on + strandat 5228.054 kb on - strandat 5228.077 kb on + strandat 5228.228 kb on - strand, within BWI76_RS25115at 5228.249 kb on + strand, within BWI76_RS25115at 5228.359 kb on + strand, within BWI76_RS25115at 5228.390 kb on + strand, within BWI76_RS25115at 5228.455 kb on + strand, within BWI76_RS25115at 5228.585 kb on - strand, within BWI76_RS25115

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM
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5,228,036 + -3.2
5,228,036 + -1.2
5,228,054 - -1.8
5,228,077 + -2.8
5,228,228 - BWI76_RS25115 0.20 -1.1
5,228,249 + BWI76_RS25115 0.22 -2.6
5,228,359 + BWI76_RS25115 0.30 -1.9
5,228,390 + BWI76_RS25115 0.33 -1.7
5,228,455 + BWI76_RS25115 0.37 -1.0
5,228,585 - BWI76_RS25115 0.47 -1.2

Or see this region's nucleotide sequence