Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS23790

Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS23780 and BWI76_RS23785 are separated by 14 nucleotidesBWI76_RS23785 and BWI76_RS23790 are separated by 52 nucleotidesBWI76_RS23790 and BWI76_RS23795 are separated by 42 nucleotides BWI76_RS23780: BWI76_RS23780 - aromatic acid decarboxylase, at 4,964,633 to 4,965,211 _RS23780 BWI76_RS23785: BWI76_RS23785 - hypothetical protein, at 4,965,226 to 4,965,942 _RS23785 BWI76_RS23790: BWI76_RS23790 - isopentenyl-diphosphate Delta-isomerase, at 4,965,995 to 4,966,543 _RS23790 BWI76_RS23795: BWI76_RS23795 - lysine--tRNA ligase, at 4,966,586 to 4,968,103 _RS23795 Position (kb) 4965 4966 4967Strain fitness (log2 ratio) -2 -1 0 1 2at 4965.163 kb on + strandat 4965.178 kb on + strandat 4965.244 kb on - strandat 4965.335 kb on - strand, within BWI76_RS23785at 4965.343 kb on - strand, within BWI76_RS23785at 4965.358 kb on - strand, within BWI76_RS23785at 4965.535 kb on - strand, within BWI76_RS23785at 4965.688 kb on + strand, within BWI76_RS23785at 4965.711 kb on - strand, within BWI76_RS23785at 4966.034 kb on - strandat 4966.036 kb on + strandat 4966.491 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM
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4,965,163 + +1.9
4,965,178 + +0.4
4,965,244 - +0.5
4,965,335 - BWI76_RS23785 0.15 -2.2
4,965,343 - BWI76_RS23785 0.16 +0.7
4,965,358 - BWI76_RS23785 0.18 -1.2
4,965,535 - BWI76_RS23785 0.43 +0.1
4,965,688 + BWI76_RS23785 0.64 +2.3
4,965,711 - BWI76_RS23785 0.68 +0.3
4,966,034 - -0.1
4,966,036 + -1.3
4,966,491 - -0.8

Or see this region's nucleotide sequence