Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS21230

Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntBWI76_RS21225 and BWI76_RS21230 overlap by 14 nucleotidesBWI76_RS21230 and BWI76_RS21235 overlap by 15 nucleotidesBWI76_RS21235 and BWI76_RS21240 overlap by 56 nucleotides BWI76_RS21225: BWI76_RS21225 - serine hydroxymethyltransferase, at 4,445,622 to 4,446,875 _RS21225 BWI76_RS21230: BWI76_RS21230 - hypothetical protein, at 4,446,862 to 4,447,091 _RS21230 BWI76_RS21235: BWI76_RS21235 - hypothetical protein, at 4,447,077 to 4,447,256 _RS21235 BWI76_RS21240: BWI76_RS21240 - flavohemoprotein, at 4,447,201 to 4,448,391 _RS21240 Position (kb) 4446 4447 4448Strain fitness (log2 ratio) -1 0 1at 4446.973 kb on - strand, within BWI76_RS21230at 4447.198 kb on + strand, within BWI76_RS21235at 4447.259 kb on - strandat 4447.357 kb on - strand, within BWI76_RS21240at 4447.482 kb on + strand, within BWI76_RS21240at 4447.482 kb on + strand, within BWI76_RS21240at 4447.584 kb on - strand, within BWI76_RS21240at 4447.854 kb on - strand, within BWI76_RS21240at 4448.039 kb on - strand, within BWI76_RS21240

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM
remove
4,446,973 - BWI76_RS21230 0.48 -1.0
4,447,198 + BWI76_RS21235 0.67 +0.3
4,447,259 - +1.0
4,447,357 - BWI76_RS21240 0.13 -0.0
4,447,482 + BWI76_RS21240 0.24 +0.7
4,447,482 + BWI76_RS21240 0.24 -0.6
4,447,584 - BWI76_RS21240 0.32 -0.0
4,447,854 - BWI76_RS21240 0.55 -0.5
4,448,039 - BWI76_RS21240 0.70 -0.2

Or see this region's nucleotide sequence