Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20645

Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20640 and BWI76_RS20645 are separated by 381 nucleotidesBWI76_RS20645 and BWI76_RS20650 are separated by 44 nucleotides BWI76_RS20640: BWI76_RS20640 - cysteine synthase A, at 4,319,208 to 4,320,179 _RS20640 BWI76_RS20645: BWI76_RS20645 - phosphocarrier protein HPr, at 4,320,561 to 4,320,818 _RS20645 BWI76_RS20650: BWI76_RS20650 - phosphoenolpyruvate--protein phosphotransferase, at 4,320,863 to 4,322,590 _RS20650 Position (kb) 4320 4321Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4 5 6at 4319.820 kb on - strand, within BWI76_RS20640at 4319.865 kb on - strand, within BWI76_RS20640at 4320.263 kb on - strandat 4320.449 kb on + strandat 4321.146 kb on - strand, within BWI76_RS20650at 4321.357 kb on + strand, within BWI76_RS20650at 4321.684 kb on + strand, within BWI76_RS20650

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM
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4,319,820 - BWI76_RS20640 0.63 +4.9
4,319,865 - BWI76_RS20640 0.68 +6.3
4,320,263 - -0.6
4,320,449 + -0.1
4,321,146 - BWI76_RS20650 0.16 -0.0
4,321,357 + BWI76_RS20650 0.29 +1.0
4,321,684 + BWI76_RS20650 0.48 -3.5

Or see this region's nucleotide sequence