Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20555

Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20545 and BWI76_RS20550 are separated by 202 nucleotidesBWI76_RS20550 and BWI76_RS20555 are separated by 41 nucleotidesBWI76_RS20555 and BWI76_RS20560 are separated by 219 nucleotidesBWI76_RS20560 and BWI76_RS20565 are separated by 4 nucleotides BWI76_RS20545: BWI76_RS20545 - sensor domain-containing phosphodiesterase, at 4,303,715 to 4,305,895 _RS20545 BWI76_RS20550: BWI76_RS20550 - tRNA-Ala, at 4,306,098 to 4,306,173 _RS20550 BWI76_RS20555: BWI76_RS20555 - tRNA-Ala, at 4,306,215 to 4,306,290 _RS20555 BWI76_RS20560: BWI76_RS20560 - hypothetical protein, at 4,306,510 to 4,306,863 _RS20560 BWI76_RS20565: BWI76_RS20565 - hypothetical protein, at 4,306,868 to 4,307,263 _RS20565 Position (kb) 4306 4307Strain fitness (log2 ratio) -2 -1 0 1 2at 4305.246 kb on - strand, within BWI76_RS20545at 4305.303 kb on - strandat 4305.303 kb on - strand, within BWI76_RS20545at 4305.389 kb on + strand, within BWI76_RS20545at 4305.474 kb on + strand, within BWI76_RS20545at 4305.474 kb on + strand, within BWI76_RS20545at 4305.474 kb on + strand, within BWI76_RS20545at 4305.474 kb on + strand, within BWI76_RS20545at 4305.474 kb on + strand, within BWI76_RS20545at 4305.474 kb on + strand, within BWI76_RS20545at 4305.474 kb on + strand, within BWI76_RS20545at 4305.474 kb on + strand, within BWI76_RS20545at 4305.474 kb on + strand, within BWI76_RS20545at 4305.474 kb on + strand, within BWI76_RS20545at 4305.474 kb on + strand, within BWI76_RS20545at 4305.474 kb on + strand, within BWI76_RS20545at 4305.474 kb on + strand, within BWI76_RS20545at 4305.474 kb on + strand, within BWI76_RS20545at 4305.474 kb on + strandat 4305.474 kb on + strand, within BWI76_RS20545at 4305.474 kb on + strand, within BWI76_RS20545at 4305.474 kb on + strand, within BWI76_RS20545at 4305.479 kb on + strand, within BWI76_RS20545at 4305.482 kb on - strand, within BWI76_RS20545at 4305.482 kb on - strand, within BWI76_RS20545at 4305.482 kb on - strand, within BWI76_RS20545at 4305.482 kb on - strand, within BWI76_RS20545at 4305.482 kb on - strand, within BWI76_RS20545at 4305.482 kb on - strand, within BWI76_RS20545at 4305.482 kb on - strand, within BWI76_RS20545at 4305.482 kb on - strand, within BWI76_RS20545at 4305.482 kb on - strand, within BWI76_RS20545at 4305.482 kb on - strand, within BWI76_RS20545at 4305.482 kb on - strand, within BWI76_RS20545at 4305.490 kb on + strand, within BWI76_RS20545at 4305.544 kb on + strand, within BWI76_RS20545at 4305.545 kb on - strandat 4305.549 kb on - strand, within BWI76_RS20545at 4305.572 kb on - strand, within BWI76_RS20545at 4305.573 kb on + strand, within BWI76_RS20545at 4305.595 kb on - strand, within BWI76_RS20545at 4305.658 kb on - strand, within BWI76_RS20545at 4305.664 kb on + strand, within BWI76_RS20545at 4305.683 kb on - strandat 4305.753 kb on - strandat 4305.753 kb on - strandat 4305.760 kb on - strandat 4305.837 kb on + strandat 4305.878 kb on - strandat 4305.956 kb on + strandat 4306.006 kb on + strandat 4306.019 kb on - strandat 4306.021 kb on - strandat 4306.344 kb on + strandat 4306.509 kb on - strandat 4306.531 kb on - strandat 4306.563 kb on - strand, within BWI76_RS20560at 4306.637 kb on - strand, within BWI76_RS20560at 4306.639 kb on - strand, within BWI76_RS20560at 4307.110 kb on - strand, within BWI76_RS20565at 4307.155 kb on - strand, within BWI76_RS20565at 4307.185 kb on - strand, within BWI76_RS20565at 4307.247 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM
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4,305,246 - BWI76_RS20545 0.70 -2.4
4,305,303 - -0.6
4,305,303 - BWI76_RS20545 0.73 -0.6
4,305,389 + BWI76_RS20545 0.77 -0.5
4,305,474 + BWI76_RS20545 0.81 -0.9
4,305,474 + BWI76_RS20545 0.81 -0.2
4,305,474 + BWI76_RS20545 0.81 +0.7
4,305,474 + BWI76_RS20545 0.81 -0.5
4,305,474 + BWI76_RS20545 0.81 -1.4
4,305,474 + BWI76_RS20545 0.81 -2.4
4,305,474 + BWI76_RS20545 0.81 -0.9
4,305,474 + BWI76_RS20545 0.81 +0.4
4,305,474 + BWI76_RS20545 0.81 -0.8
4,305,474 + BWI76_RS20545 0.81 +1.2
4,305,474 + BWI76_RS20545 0.81 +0.2
4,305,474 + BWI76_RS20545 0.81 -0.2
4,305,474 + BWI76_RS20545 0.81 +1.0
4,305,474 + BWI76_RS20545 0.81 +2.0
4,305,474 + +0.4
4,305,474 + BWI76_RS20545 0.81 +0.4
4,305,474 + BWI76_RS20545 0.81 -0.0
4,305,474 + BWI76_RS20545 0.81 +0.2
4,305,479 + BWI76_RS20545 0.81 +0.1
4,305,482 - BWI76_RS20545 0.81 -1.4
4,305,482 - BWI76_RS20545 0.81 +0.7
4,305,482 - BWI76_RS20545 0.81 +1.0
4,305,482 - BWI76_RS20545 0.81 -1.4
4,305,482 - BWI76_RS20545 0.81 -0.0
4,305,482 - BWI76_RS20545 0.81 -1.0
4,305,482 - BWI76_RS20545 0.81 +0.7
4,305,482 - BWI76_RS20545 0.81 +0.1
4,305,482 - BWI76_RS20545 0.81 -0.0
4,305,482 - BWI76_RS20545 0.81 +0.3
4,305,482 - BWI76_RS20545 0.81 +0.1
4,305,490 + BWI76_RS20545 0.81 -0.4
4,305,544 + BWI76_RS20545 0.84 -0.8
4,305,545 - +0.4
4,305,549 - BWI76_RS20545 0.84 +0.8
4,305,572 - BWI76_RS20545 0.85 +0.7
4,305,573 + BWI76_RS20545 0.85 +0.1
4,305,595 - BWI76_RS20545 0.86 -0.0
4,305,658 - BWI76_RS20545 0.89 -0.1
4,305,664 + BWI76_RS20545 0.89 -0.7
4,305,683 - -0.4
4,305,753 - -0.1
4,305,753 - -0.7
4,305,760 - -0.3
4,305,837 + +0.6
4,305,878 - +1.4
4,305,956 + -0.2
4,306,006 + -0.6
4,306,019 - -1.0
4,306,021 - +2.0
4,306,344 + -0.6
4,306,509 - -0.4
4,306,531 - -0.3
4,306,563 - BWI76_RS20560 0.15 -1.8
4,306,637 - BWI76_RS20560 0.36 -0.9
4,306,639 - BWI76_RS20560 0.36 +0.8
4,307,110 - BWI76_RS20565 0.61 +1.3
4,307,155 - BWI76_RS20565 0.72 -0.2
4,307,185 - BWI76_RS20565 0.80 +0.4
4,307,247 - -0.5

Or see this region's nucleotide sequence