Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20535

Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20525 and BWI76_RS20530 are separated by 130 nucleotidesBWI76_RS20530 and BWI76_RS20535 are separated by 48 nucleotidesBWI76_RS20535 and BWI76_RS20540 are separated by 348 nucleotides BWI76_RS20525: BWI76_RS20525 - glyceraldehyde 3-phosphate reductase, at 4,299,367 to 4,300,359 _RS20525 BWI76_RS20530: BWI76_RS20530 - hypothetical protein, at 4,300,490 to 4,300,849 _RS20530 BWI76_RS20535: BWI76_RS20535 - manganese transporter MntH, at 4,300,898 to 4,302,133 _RS20535 BWI76_RS20540: BWI76_RS20540 - NupC/NupG family nucleoside CNT transporter, at 4,302,482 to 4,303,684 _RS20540 Position (kb) 4300 4301 4302 4303Strain fitness (log2 ratio) -2 -1 0 1 2 3at 4299.979 kb on - strand, within BWI76_RS20525at 4300.019 kb on + strand, within BWI76_RS20525at 4300.207 kb on + strand, within BWI76_RS20525at 4300.215 kb on - strand, within BWI76_RS20525at 4300.215 kb on - strand, within BWI76_RS20525at 4300.241 kb on + strand, within BWI76_RS20525at 4300.275 kb on + strandat 4300.417 kb on - strandat 4300.434 kb on + strandat 4300.447 kb on + strandat 4300.488 kb on - strandat 4300.563 kb on + strand, within BWI76_RS20530at 4300.704 kb on - strand, within BWI76_RS20530at 4300.726 kb on + strand, within BWI76_RS20530at 4301.402 kb on + strand, within BWI76_RS20535at 4302.305 kb on - strandat 4302.307 kb on + strandat 4302.479 kb on + strandat 4302.479 kb on + strandat 4302.646 kb on - strand, within BWI76_RS20540at 4302.737 kb on + strand, within BWI76_RS20540at 4302.854 kb on - strand, within BWI76_RS20540at 4302.889 kb on + strand, within BWI76_RS20540

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM
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4,299,979 - BWI76_RS20525 0.62 +0.4
4,300,019 + BWI76_RS20525 0.66 +0.4
4,300,207 + BWI76_RS20525 0.85 +0.1
4,300,215 - BWI76_RS20525 0.85 -0.2
4,300,215 - BWI76_RS20525 0.85 -0.3
4,300,241 + BWI76_RS20525 0.88 +1.7
4,300,275 + +0.2
4,300,417 - +2.8
4,300,434 + -0.4
4,300,447 + -0.8
4,300,488 - -0.7
4,300,563 + BWI76_RS20530 0.20 -0.8
4,300,704 - BWI76_RS20530 0.59 -0.4
4,300,726 + BWI76_RS20530 0.66 +0.8
4,301,402 + BWI76_RS20535 0.41 -0.4
4,302,305 - +1.0
4,302,307 + +1.0
4,302,479 + -0.3
4,302,479 + +0.6
4,302,646 - BWI76_RS20540 0.14 -0.6
4,302,737 + BWI76_RS20540 0.21 +0.2
4,302,854 - BWI76_RS20540 0.31 -2.0
4,302,889 + BWI76_RS20540 0.34 -0.4

Or see this region's nucleotide sequence