Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS19005

Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS19000 and BWI76_RS19005 are separated by 225 nucleotidesBWI76_RS19005 and BWI76_RS19010 overlap by 4 nucleotidesBWI76_RS19010 and BWI76_RS19015 are separated by 16 nucleotides BWI76_RS19000: BWI76_RS19000 - acyltransferase, at 3,943,705 to 3,944,826 _RS19000 BWI76_RS19005: BWI76_RS19005 - capsular polysaccharide biosynthesis protein, at 3,945,052 to 3,946,038 _RS19005 BWI76_RS19010: BWI76_RS19010 - polysaccharide pyruvyl transferase yvff, at 3,946,035 to 3,946,982 _RS19010 BWI76_RS19015: BWI76_RS19015 - lipopolysaccharide biosynthesis protein, at 3,946,999 to 3,948,471 _RS19015 Position (kb) 3945 3946 3947Strain fitness (log2 ratio) -2 -1 0 1 2 3at 3944.068 kb on + strand, within BWI76_RS19000at 3944.089 kb on - strand, within BWI76_RS19000at 3944.168 kb on - strand, within BWI76_RS19000at 3944.626 kb on - strand, within BWI76_RS19000at 3944.692 kb on + strand, within BWI76_RS19000at 3944.695 kb on - strand, within BWI76_RS19000at 3944.699 kb on + strand, within BWI76_RS19000at 3944.748 kb on - strandat 3944.764 kb on - strandat 3944.839 kb on - strandat 3944.847 kb on - strandat 3944.847 kb on - strandat 3944.847 kb on - strandat 3944.847 kb on - strandat 3944.847 kb on - strandat 3944.847 kb on - strandat 3944.847 kb on - strandat 3944.847 kb on - strandat 3944.847 kb on - strandat 3944.847 kb on - strandat 3944.847 kb on - strandat 3944.847 kb on - strandat 3944.847 kb on - strandat 3944.847 kb on - strandat 3944.847 kb on - strandat 3944.847 kb on - strandat 3944.847 kb on - strandat 3944.847 kb on - strandat 3944.847 kb on - strandat 3944.847 kb on - strandat 3944.847 kb on - strandat 3944.847 kb on - strandat 3944.847 kb on - strandat 3944.847 kb on - strandat 3944.847 kb on - strandat 3944.847 kb on - strandat 3944.847 kb on - strandat 3944.847 kb on - strandat 3944.847 kb on - strandat 3944.847 kb on - strandat 3944.847 kb on - strandat 3944.847 kb on - strandat 3944.847 kb on - strandat 3944.847 kb on - strandat 3944.847 kb on - strandat 3944.847 kb on - strandat 3944.847 kb on - strandat 3944.847 kb on - strandat 3944.847 kb on - strandat 3944.847 kb on - strandat 3944.847 kb on - strandat 3944.847 kb on - strandat 3944.847 kb on - strandat 3944.847 kb on - strandat 3944.847 kb on - strandat 3944.847 kb on - strandat 3944.847 kb on - strandat 3944.847 kb on - strandat 3944.847 kb on - strandat 3944.847 kb on - strandat 3944.847 kb on - strandat 3944.847 kb on - strandat 3944.847 kb on - strandat 3944.847 kb on - strandat 3944.847 kb on - strandat 3944.891 kb on - strandat 3945.088 kb on - strandat 3945.299 kb on - strand, within BWI76_RS19005at 3945.372 kb on - strand, within BWI76_RS19005at 3946.074 kb on - strandat 3946.151 kb on + strand, within BWI76_RS19010at 3946.241 kb on - strand, within BWI76_RS19010at 3946.243 kb on - strand, within BWI76_RS19010at 3946.435 kb on - strand, within BWI76_RS19010at 3946.435 kb on - strand, within BWI76_RS19010at 3946.504 kb on - strand, within BWI76_RS19010at 3946.986 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM
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3,944,068 + BWI76_RS19000 0.32 +0.2
3,944,089 - BWI76_RS19000 0.34 +0.4
3,944,168 - BWI76_RS19000 0.41 -0.8
3,944,626 - BWI76_RS19000 0.82 +0.2
3,944,692 + BWI76_RS19000 0.88 +0.0
3,944,695 - BWI76_RS19000 0.88 -0.8
3,944,699 + BWI76_RS19000 0.89 -0.9
3,944,748 - +0.2
3,944,764 - +3.6
3,944,839 - -0.8
3,944,847 - -1.0
3,944,847 - +0.3
3,944,847 - -0.6
3,944,847 - -0.6
3,944,847 - -1.7
3,944,847 - -1.4
3,944,847 - +0.8
3,944,847 - +0.3
3,944,847 - +0.7
3,944,847 - +0.1
3,944,847 - +0.1
3,944,847 - -0.6
3,944,847 - +0.3
3,944,847 - +0.2
3,944,847 - -0.9
3,944,847 - -1.9
3,944,847 - +0.5
3,944,847 - +0.6
3,944,847 - -0.8
3,944,847 - -1.1
3,944,847 - -0.7
3,944,847 - +0.0
3,944,847 - -0.2
3,944,847 - +0.9
3,944,847 - +1.0
3,944,847 - -0.1
3,944,847 - -1.8
3,944,847 - +0.1
3,944,847 - +0.3
3,944,847 - -1.3
3,944,847 - -0.2
3,944,847 - -1.1
3,944,847 - -0.1
3,944,847 - -1.6
3,944,847 - -0.3
3,944,847 - +1.4
3,944,847 - -1.6
3,944,847 - +1.8
3,944,847 - +0.8
3,944,847 - -0.1
3,944,847 - -0.7
3,944,847 - +1.6
3,944,847 - -0.4
3,944,847 - +0.1
3,944,847 - -0.8
3,944,847 - +0.9
3,944,847 - -0.1
3,944,847 - -0.8
3,944,847 - -0.6
3,944,847 - -0.1
3,944,847 - -0.1
3,944,847 - +0.1
3,944,847 - +0.2
3,944,847 - -0.6
3,944,847 - +2.3
3,944,891 - -0.2
3,945,088 - +0.1
3,945,299 - BWI76_RS19005 0.25 -1.6
3,945,372 - BWI76_RS19005 0.32 -2.0
3,946,074 - +0.5
3,946,151 + BWI76_RS19010 0.12 -1.6
3,946,241 - BWI76_RS19010 0.22 -0.5
3,946,243 - BWI76_RS19010 0.22 -1.0
3,946,435 - BWI76_RS19010 0.42 +0.3
3,946,435 - BWI76_RS19010 0.42 -2.3
3,946,504 - BWI76_RS19010 0.49 -2.1
3,946,986 - -0.1

Or see this region's nucleotide sequence