Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS14315

Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS14305 and BWI76_RS14310 are separated by 4 nucleotidesBWI76_RS14310 and BWI76_RS14315 overlap by 1 nucleotidesBWI76_RS14315 and BWI76_RS14320 are separated by 1 nucleotidesBWI76_RS14320 and BWI76_RS14325 are separated by 7 nucleotides BWI76_RS14305: BWI76_RS14305 - metallophosphoesterase, at 2,989,883 to 2,990,668 _RS14305 BWI76_RS14310: BWI76_RS14310 - hypothetical protein, at 2,990,673 to 2,991,554 _RS14310 BWI76_RS14315: BWI76_RS14315 - MazG nucleotide pyrophosphohydrolase, at 2,991,554 to 2,991,892 _RS14315 BWI76_RS14320: BWI76_RS14320 - hypothetical protein, at 2,991,894 to 2,992,829 _RS14320 BWI76_RS14325: BWI76_RS14325 - maturase, at 2,992,837 to 2,992,957 _RS14325 Position (kb) 2991 2992Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 2990.838 kb on + strand, within BWI76_RS14310at 2990.838 kb on + strand, within BWI76_RS14310at 2990.840 kb on + strand, within BWI76_RS14310at 2990.840 kb on + strandat 2990.848 kb on + strand, within BWI76_RS14310at 2990.850 kb on + strand, within BWI76_RS14310at 2990.985 kb on + strand, within BWI76_RS14310at 2991.214 kb on + strand, within BWI76_RS14310at 2991.275 kb on + strand, within BWI76_RS14310at 2991.275 kb on + strand, within BWI76_RS14310at 2991.275 kb on + strand, within BWI76_RS14310at 2991.294 kb on + strand, within BWI76_RS14310at 2991.307 kb on + strandat 2991.320 kb on + strand, within BWI76_RS14310at 2991.326 kb on + strand, within BWI76_RS14310at 2991.327 kb on + strand, within BWI76_RS14310at 2991.344 kb on + strand, within BWI76_RS14310at 2991.350 kb on + strand, within BWI76_RS14310at 2991.374 kb on + strand, within BWI76_RS14310at 2991.374 kb on + strand, within BWI76_RS14310at 2991.452 kb on + strand, within BWI76_RS14310at 2991.491 kb on + strandat 2991.491 kb on + strandat 2991.491 kb on + strandat 2991.491 kb on + strandat 2991.491 kb on + strandat 2991.491 kb on + strandat 2991.491 kb on + strandat 2991.498 kb on + strandat 2991.498 kb on + strandat 2991.498 kb on + strandat 2991.498 kb on + strandat 2991.498 kb on + strandat 2991.498 kb on + strandat 2992.011 kb on + strand, within BWI76_RS14320at 2992.114 kb on + strand, within BWI76_RS14320at 2992.201 kb on + strand, within BWI76_RS14320at 2992.293 kb on + strandat 2992.293 kb on + strand, within BWI76_RS14320at 2992.395 kb on + strand, within BWI76_RS14320at 2992.830 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM
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2,990,838 + BWI76_RS14310 0.19 -0.3
2,990,838 + BWI76_RS14310 0.19 -1.0
2,990,840 + BWI76_RS14310 0.19 -0.2
2,990,840 + +2.1
2,990,848 + BWI76_RS14310 0.20 -0.9
2,990,850 + BWI76_RS14310 0.20 -0.8
2,990,985 + BWI76_RS14310 0.35 +0.7
2,991,214 + BWI76_RS14310 0.61 +0.2
2,991,275 + BWI76_RS14310 0.68 +0.2
2,991,275 + BWI76_RS14310 0.68 -0.2
2,991,275 + BWI76_RS14310 0.68 -0.8
2,991,294 + BWI76_RS14310 0.70 -0.4
2,991,307 + -0.2
2,991,320 + BWI76_RS14310 0.73 -0.0
2,991,326 + BWI76_RS14310 0.74 -1.1
2,991,327 + BWI76_RS14310 0.74 +0.4
2,991,344 + BWI76_RS14310 0.76 -1.5
2,991,350 + BWI76_RS14310 0.77 +0.5
2,991,374 + BWI76_RS14310 0.79 -1.2
2,991,374 + BWI76_RS14310 0.79 +0.0
2,991,452 + BWI76_RS14310 0.88 +0.3
2,991,491 + -0.4
2,991,491 + -0.5
2,991,491 + +1.1
2,991,491 + -0.2
2,991,491 + +2.4
2,991,491 + -2.8
2,991,491 + -0.9
2,991,498 + -1.1
2,991,498 + +0.7
2,991,498 + -0.8
2,991,498 + -0.7
2,991,498 + -0.3
2,991,498 + -4.4
2,992,011 + BWI76_RS14320 0.12 -0.3
2,992,114 + BWI76_RS14320 0.24 +1.0
2,992,201 + BWI76_RS14320 0.33 +1.0
2,992,293 + +0.5
2,992,293 + BWI76_RS14320 0.43 +1.5
2,992,395 + BWI76_RS14320 0.54 -0.2
2,992,830 + +0.7

Or see this region's nucleotide sequence