Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS10960

Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS10950 and BWI76_RS10955 overlap by 95 nucleotidesBWI76_RS10955 and BWI76_RS10960 overlap by 38 nucleotidesBWI76_RS10960 and BWI76_RS10965 are separated by 209 nucleotides BWI76_RS10950: BWI76_RS10950 - MFS transporter, at 2,312,436 to 2,313,686 _RS10950 BWI76_RS10955: BWI76_RS10955 - hypothetical protein, at 2,313,592 to 2,313,899 _RS10955 BWI76_RS10960: BWI76_RS10960 - lipid A biosynthesis lauroyl acyltransferase, at 2,313,862 to 2,314,782 _RS10960 BWI76_RS10965: BWI76_RS10965 - sulfurtransferase, at 2,314,992 to 2,316,056 _RS10965 Position (kb) 2313 2314 2315Strain fitness (log2 ratio) -1 0 1 2 3at 2313.263 kb on + strand, within BWI76_RS10950at 2313.339 kb on + strand, within BWI76_RS10950at 2313.356 kb on + strand, within BWI76_RS10950at 2313.769 kb on + strand, within BWI76_RS10955at 2313.844 kb on - strand, within BWI76_RS10955at 2314.790 kb on - strandat 2314.790 kb on - strandat 2314.790 kb on - strandat 2314.931 kb on - strandat 2314.984 kb on + strandat 2314.992 kb on - strandat 2315.034 kb on - strandat 2315.050 kb on + strandat 2315.050 kb on + strandat 2315.058 kb on - strandat 2315.121 kb on + strandat 2315.138 kb on + strand, within BWI76_RS10965at 2315.151 kb on + strand, within BWI76_RS10965at 2315.205 kb on + strand, within BWI76_RS10965at 2315.306 kb on + strand, within BWI76_RS10965at 2315.320 kb on + strand, within BWI76_RS10965

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM
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2,313,263 + BWI76_RS10950 0.66 +1.5
2,313,339 + BWI76_RS10950 0.72 -0.4
2,313,356 + BWI76_RS10950 0.74 -0.7
2,313,769 + BWI76_RS10955 0.57 -0.0
2,313,844 - BWI76_RS10955 0.82 -0.3
2,314,790 - -0.5
2,314,790 - +0.1
2,314,790 - +0.5
2,314,931 - -1.1
2,314,984 + -0.4
2,314,992 - -0.1
2,315,034 - +0.1
2,315,050 + -0.5
2,315,050 + -0.3
2,315,058 - +0.4
2,315,121 + +3.0
2,315,138 + BWI76_RS10965 0.14 -1.0
2,315,151 + BWI76_RS10965 0.15 +0.8
2,315,205 + BWI76_RS10965 0.20 -0.1
2,315,306 + BWI76_RS10965 0.29 -0.3
2,315,320 + BWI76_RS10965 0.31 -0.4

Or see this region's nucleotide sequence