Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS10425

Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS10420 and BWI76_RS10425 are separated by 43 nucleotidesBWI76_RS10425 and BWI76_RS10430 are separated by 107 nucleotidesBWI76_RS10430 and BWI76_RS10435 are separated by 44 nucleotides BWI76_RS10420: BWI76_RS10420 - monooxygenase, at 2,203,362 to 2,204,390 _RS10420 BWI76_RS10425: BWI76_RS10425 - AIG2 family protein, at 2,204,434 to 2,204,769 _RS10425 BWI76_RS10430: BWI76_RS10430 - thiol:disulfide interchange protein DsbG, at 2,204,877 to 2,205,635 _RS10430 BWI76_RS10435: BWI76_RS10435 - suppressor for copper-sensitivity C, at 2,205,680 to 2,206,402 _RS10435 Position (kb) 2204 2205Strain fitness (log2 ratio) -1 0 1 2at 2203.625 kb on + strand, within BWI76_RS10420at 2203.633 kb on - strand, within BWI76_RS10420at 2203.633 kb on - strand, within BWI76_RS10420at 2203.672 kb on + strand, within BWI76_RS10420at 2203.729 kb on + strand, within BWI76_RS10420at 2203.880 kb on + strand, within BWI76_RS10420at 2204.239 kb on - strand, within BWI76_RS10420at 2204.329 kb on - strandat 2204.744 kb on - strandat 2205.247 kb on + strand, within BWI76_RS10430at 2205.360 kb on + strand, within BWI76_RS10430at 2205.445 kb on + strand, within BWI76_RS10430at 2205.491 kb on - strand, within BWI76_RS10430at 2205.589 kb on - strandat 2205.739 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM
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2,203,625 + BWI76_RS10420 0.26 -0.3
2,203,633 - BWI76_RS10420 0.26 -0.1
2,203,633 - BWI76_RS10420 0.26 +0.9
2,203,672 + BWI76_RS10420 0.30 -0.7
2,203,729 + BWI76_RS10420 0.36 -0.4
2,203,880 + BWI76_RS10420 0.50 -0.1
2,204,239 - BWI76_RS10420 0.85 -0.2
2,204,329 - +2.0
2,204,744 - +1.4
2,205,247 + BWI76_RS10430 0.49 +0.3
2,205,360 + BWI76_RS10430 0.64 -0.5
2,205,445 + BWI76_RS10430 0.75 -0.5
2,205,491 - BWI76_RS10430 0.81 +0.6
2,205,589 - +1.4
2,205,739 - -0.1

Or see this region's nucleotide sequence