Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS09770

Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS09760 and BWI76_RS09765 are separated by 147 nucleotidesBWI76_RS09765 and BWI76_RS09770 overlap by 54 nucleotidesBWI76_RS09770 and BWI76_RS09775 overlap by 68 nucleotidesBWI76_RS09775 and BWI76_RS09780 are separated by 137 nucleotides BWI76_RS09760: BWI76_RS09760 - 3-phosphoshikimate 1-carboxyvinyltransferase, at 2,047,316 to 2,048,599 _RS09760 BWI76_RS09765: BWI76_RS09765 - metalloprotease, at 2,048,747 to 2,049,514 _RS09765 BWI76_RS09770: BWI76_RS09770 - hypothetical protein, at 2,049,461 to 2,049,753 _RS09770 BWI76_RS09775: BWI76_RS09775 - cytidylate kinase, at 2,049,686 to 2,050,369 _RS09775 BWI76_RS09780: BWI76_RS09780 - 30S ribosomal protein S1, at 2,050,507 to 2,052,180 _RS09780 Position (kb) 2049 2050Strain fitness (log2 ratio) -2 -1 0 1at 2048.593 kb on + strandat 2048.593 kb on + strandat 2048.837 kb on - strand, within BWI76_RS09765at 2049.152 kb on - strand, within BWI76_RS09765at 2049.624 kb on - strand, within BWI76_RS09770

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM
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2,048,593 + +0.1
2,048,593 + -2.5
2,048,837 - BWI76_RS09765 0.12 +0.0
2,049,152 - BWI76_RS09765 0.53 +0.1
2,049,624 - BWI76_RS09770 0.56 -0.6

Or see this region's nucleotide sequence