Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS08155

Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS08140 and BWI76_RS08145 are separated by 109 nucleotidesBWI76_RS08145 and BWI76_RS08150 are separated by 97 nucleotidesBWI76_RS08150 and BWI76_RS08155 are separated by 45 nucleotidesBWI76_RS08155 and BWI76_RS08160 are separated by 21 nucleotidesBWI76_RS08160 and BWI76_RS08165 are separated by 34 nucleotidesBWI76_RS08165 and BWI76_RS08170 are separated by 35 nucleotidesBWI76_RS08170 and BWI76_RS08175 are separated by 8 nucleotidesBWI76_RS08175 and BWI76_RS08180 are separated by 229 nucleotides BWI76_RS08140: BWI76_RS08140 - 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase, at 1,725,763 to 1,726,938 _RS08140 BWI76_RS08145: BWI76_RS08145 - tRNA-Gln, at 1,727,048 to 1,727,122 _RS08145 BWI76_RS08150: BWI76_RS08150 - tRNA-Gln, at 1,727,220 to 1,727,294 _RS08150 BWI76_RS08155: BWI76_RS08155 - tRNA-Met, at 1,727,340 to 1,727,416 _RS08155 BWI76_RS08160: BWI76_RS08160 - tRNA-Gln, at 1,727,438 to 1,727,512 _RS08160 BWI76_RS08165: BWI76_RS08165 - tRNA-Gln, at 1,727,547 to 1,727,621 _RS08165 BWI76_RS08170: BWI76_RS08170 - tRNA-Leu, at 1,727,657 to 1,727,741 _RS08170 BWI76_RS08175: BWI76_RS08175 - tRNA-Met, at 1,727,750 to 1,727,826 _RS08175 BWI76_RS08180: BWI76_RS08180 - asparagine synthase B, at 1,728,056 to 1,729,717 _RS08180 Position (kb) 1727 1728Strain fitness (log2 ratio) -2 -1 0 1at 1726.982 kb on - strandat 1728.054 kb on + strandat 1728.224 kb on + strand, within BWI76_RS08180

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM
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1,726,982 - -0.4
1,728,054 + -2.0
1,728,224 + BWI76_RS08180 0.10 -0.7

Or see this region's nucleotide sequence