Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS04190

Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS04185 and BWI76_RS04190 are separated by 117 nucleotidesBWI76_RS04190 and BWI76_RS04195 overlap by 20 nucleotidesBWI76_RS04195 and BWI76_RS04200 are separated by 114 nucleotides BWI76_RS04185: BWI76_RS04185 - murein transglycosylase, at 861,329 to 863,266 _RS04185 BWI76_RS04190: BWI76_RS04190 - Trp operon repressor, at 863,384 to 863,716 _RS04190 BWI76_RS04195: BWI76_RS04195 - non-canonical purine NTP phosphatase, at 863,697 to 864,218 _RS04195 BWI76_RS04200: BWI76_RS04200 - phosphoglycerate mutase GpmB, at 864,333 to 864,980 _RS04200 Position (kb) 863 864Strain fitness (log2 ratio) -1 0 1 2at 862.458 kb on + strand, within BWI76_RS04185at 862.495 kb on + strand, within BWI76_RS04185at 862.695 kb on - strand, within BWI76_RS04185at 863.062 kb on - strand, within BWI76_RS04185at 863.231 kb on + strandat 863.241 kb on - strandat 863.283 kb on - strandat 864.304 kb on + strandat 864.305 kb on - strandat 864.313 kb on - strandat 864.581 kb on + strand, within BWI76_RS04200at 864.589 kb on - strand, within BWI76_RS04200

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM
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862,458 + BWI76_RS04185 0.58 -0.4
862,495 + BWI76_RS04185 0.60 -0.5
862,695 - BWI76_RS04185 0.70 +0.4
863,062 - BWI76_RS04185 0.89 -0.2
863,231 + -0.3
863,241 - -0.3
863,283 - +0.6
864,304 + +0.2
864,305 - -0.5
864,313 - -0.5
864,581 + BWI76_RS04200 0.38 -0.0
864,589 - BWI76_RS04200 0.40 +2.0

Or see this region's nucleotide sequence