Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS02675

Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS02660 and BWI76_RS02665 are separated by 5 nucleotidesBWI76_RS02665 and BWI76_RS02670 are separated by 4 nucleotidesBWI76_RS02670 and BWI76_RS02675 are separated by 41 nucleotidesBWI76_RS02675 and BWI76_RS02680 are separated by 146 nucleotides BWI76_RS02660: BWI76_RS02660 - 30S ribosomal protein S6, at 542,473 to 542,868 _RS02660 BWI76_RS02665: BWI76_RS02665 - primosomal replication protein N, at 542,874 to 543,188 _RS02665 BWI76_RS02670: BWI76_RS02670 - 30S ribosomal protein S18, at 543,193 to 543,420 _RS02670 BWI76_RS02675: BWI76_RS02675 - 50S ribosomal protein L9, at 543,462 to 543,911 _RS02675 BWI76_RS02680: BWI76_RS02680 - EamA family transporter, at 544,058 to 545,011 _RS02680 Position (kb) 543 544Strain fitness (log2 ratio) -2 -1 0 1 2at 543.723 kb on + strand, within BWI76_RS02675at 544.109 kb on + strandat 544.262 kb on + strand, within BWI76_RS02680at 544.275 kb on - strand, within BWI76_RS02680at 544.367 kb on - strand, within BWI76_RS02680at 544.880 kb on + strand, within BWI76_RS02680

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM
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543,723 + BWI76_RS02675 0.58 -2.6
544,109 + +0.4
544,262 + BWI76_RS02680 0.21 +2.2
544,275 - BWI76_RS02680 0.23 -0.3
544,367 - BWI76_RS02680 0.32 +0.0
544,880 + BWI76_RS02680 0.86 -0.9

Or see this region's nucleotide sequence