Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS01145

Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS01140 and BWI76_RS01145 are separated by 102 nucleotidesBWI76_RS01145 and BWI76_RS01150 are separated by 55 nucleotidesBWI76_RS01150 and BWI76_RS01155 are separated by 22 nucleotidesBWI76_RS01155 and BWI76_RS01160 are separated by 45 nucleotidesBWI76_RS01160 and BWI76_RS01165 are separated by 461 nucleotides BWI76_RS01140: BWI76_RS01140 - amino acid permease, at 235,238 to 236,623 _RS01140 BWI76_RS01145: BWI76_RS01145 - tRNA-Arg, at 236,726 to 236,802 _RS01145 BWI76_RS01150: BWI76_RS01150 - tRNA-His, at 236,858 to 236,933 _RS01150 BWI76_RS01155: BWI76_RS01155 - tRNA-Leu, at 236,956 to 237,042 _RS01155 BWI76_RS01160: BWI76_RS01160 - tRNA-Pro, at 237,088 to 237,164 _RS01160 BWI76_RS01165: BWI76_RS01165 - protoheme IX biogenesis protein HemY, at 237,626 to 238,822 _RS01165 Position (kb) 236 237Strain fitness (log2 ratio) -2 -1 0 1at 235.742 kb on - strand, within BWI76_RS01140at 235.755 kb on + strand, within BWI76_RS01140at 235.869 kb on + strand, within BWI76_RS01140at 236.038 kb on - strand, within BWI76_RS01140at 236.051 kb on + strand, within BWI76_RS01140at 236.144 kb on + strand, within BWI76_RS01140at 236.152 kb on - strand, within BWI76_RS01140at 236.154 kb on - strand, within BWI76_RS01140at 236.297 kb on - strand, within BWI76_RS01140at 236.331 kb on - strand, within BWI76_RS01140at 236.497 kb on + strandat 236.556 kb on - strandat 237.382 kb on - strandat 237.411 kb on + strandat 237.651 kb on + strandat 237.742 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM
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235,742 - BWI76_RS01140 0.36 -1.2
235,755 + BWI76_RS01140 0.37 -0.6
235,869 + BWI76_RS01140 0.46 +0.6
236,038 - BWI76_RS01140 0.58 +0.2
236,051 + BWI76_RS01140 0.59 -0.6
236,144 + BWI76_RS01140 0.65 -2.0
236,152 - BWI76_RS01140 0.66 +0.5
236,154 - BWI76_RS01140 0.66 -0.4
236,297 - BWI76_RS01140 0.76 -0.2
236,331 - BWI76_RS01140 0.79 +1.3
236,497 + -0.1
236,556 - -1.0
237,382 - -0.4
237,411 + -0.6
237,651 + -0.6
237,742 - +0.1

Or see this region's nucleotide sequence