Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS32635

Experiment: Valeric (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS32625 and RR42_RS32630 are separated by 24 nucleotidesRR42_RS32630 and RR42_RS32635 are separated by 20 nucleotidesRR42_RS32635 and RR42_RS32640 are separated by 38 nucleotidesRR42_RS32640 and RR42_RS32645 are separated by 32 nucleotides RR42_RS32625: RR42_RS32625 - 4-hyroxy-2-oxovalerate aldolase, at 2,717,513 to 2,718,562 _RS32625 RR42_RS32630: RR42_RS32630 - acetaldehyde dehydrogenase, at 2,718,587 to 2,719,531 _RS32630 RR42_RS32635: RR42_RS32635 - 4-oxalocrotonate tautomerase, at 2,719,552 to 2,719,743 _RS32635 RR42_RS32640: RR42_RS32640 - 4-oxalocrotonate decarboxylase, at 2,719,782 to 2,720,570 _RS32640 RR42_RS32645: RR42_RS32645 - 2-keto-4-pentenoate hydratase, at 2,720,603 to 2,721,385 _RS32645 Position (kb) 2719 2720Strain fitness (log2 ratio) -1 0 1at 2718.666 kb on + strandat 2718.666 kb on + strandat 2718.666 kb on + strandat 2718.667 kb on - strandat 2718.748 kb on + strand, within RR42_RS32630at 2718.816 kb on + strand, within RR42_RS32630at 2718.816 kb on + strand, within RR42_RS32630at 2718.816 kb on + strand, within RR42_RS32630at 2718.817 kb on - strand, within RR42_RS32630at 2718.817 kb on - strand, within RR42_RS32630at 2719.072 kb on - strand, within RR42_RS32630at 2719.141 kb on + strand, within RR42_RS32630at 2719.141 kb on + strand, within RR42_RS32630at 2719.141 kb on + strand, within RR42_RS32630at 2719.142 kb on - strand, within RR42_RS32630at 2719.142 kb on - strand, within RR42_RS32630at 2719.194 kb on + strand, within RR42_RS32630at 2719.194 kb on + strand, within RR42_RS32630at 2720.589 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Valeric (C)
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2,718,666 + +0.6
2,718,666 + +0.4
2,718,666 + -0.8
2,718,667 - -1.5
2,718,748 + RR42_RS32630 0.17 +0.3
2,718,816 + RR42_RS32630 0.24 +0.6
2,718,816 + RR42_RS32630 0.24 +1.3
2,718,816 + RR42_RS32630 0.24 -0.9
2,718,817 - RR42_RS32630 0.24 +0.0
2,718,817 - RR42_RS32630 0.24 +0.1
2,719,072 - RR42_RS32630 0.51 +0.4
2,719,141 + RR42_RS32630 0.59 -1.0
2,719,141 + RR42_RS32630 0.59 +0.1
2,719,141 + RR42_RS32630 0.59 +0.3
2,719,142 - RR42_RS32630 0.59 +0.4
2,719,142 - RR42_RS32630 0.59 -0.3
2,719,194 + RR42_RS32630 0.64 -0.6
2,719,194 + RR42_RS32630 0.64 -0.3
2,720,589 + -0.5

Or see this region's nucleotide sequence