Experiment: Valeric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02715 and RR42_RS02720 are separated by 526 nucleotides RR42_RS02720 and RR42_RS02725 are separated by 17 nucleotides RR42_RS02725 and RR42_RS02730 overlap by 4 nucleotides
RR42_RS02715: RR42_RS02715 - Holliday junction DNA helicase, at 605,001 to 605,546
_RS02715
RR42_RS02720: RR42_RS02720 - phosphoribosylaminoimidazolecarboxamide formyltransferase, at 606,073 to 607,647
_RS02720
RR42_RS02725: RR42_RS02725 - Fis family transcriptional regulator, at 607,665 to 607,898
_RS02725
RR42_RS02730: RR42_RS02730 - tRNA-dihydrouridine synthase B, at 607,895 to 608,989
_RS02730
Position (kb)
606
607
608 Strain fitness (log2 ratio)
-1
0
1
2 at 605.587 kb on + strand at 605.587 kb on + strand at 605.587 kb on + strand at 605.587 kb on + strand at 605.588 kb on - strand at 605.588 kb on - strand at 605.588 kb on - strand at 605.602 kb on - strand at 605.608 kb on + strand at 605.609 kb on - strand at 605.614 kb on + strand at 605.615 kb on - strand at 605.615 kb on - strand at 607.652 kb on - strand at 607.919 kb on - strand at 608.119 kb on - strand, within RR42_RS02730 at 608.167 kb on - strand, within RR42_RS02730 at 608.167 kb on - strand, within RR42_RS02730 at 608.176 kb on - strand, within RR42_RS02730 at 608.257 kb on - strand, within RR42_RS02730 at 608.428 kb on - strand, within RR42_RS02730 at 608.446 kb on - strand, within RR42_RS02730
Per-strain Table
Position Strand Gene LocusTag Fraction Valeric (C) remove 605,587 + -0.9 605,587 + -0.6 605,587 + -0.7 605,587 + -0.2 605,588 - -0.5 605,588 - -1.2 605,588 - +0.6 605,602 - +0.4 605,608 + -0.5 605,609 - -1.6 605,614 + -1.1 605,615 - -0.5 605,615 - +0.6 607,652 - -0.4 607,919 - +1.2 608,119 - RR42_RS02730 0.20 +0.1 608,167 - RR42_RS02730 0.25 -0.2 608,167 - RR42_RS02730 0.25 -0.9 608,176 - RR42_RS02730 0.26 -0.4 608,257 - RR42_RS02730 0.33 -0.6 608,428 - RR42_RS02730 0.49 +1.0 608,446 - RR42_RS02730 0.50 +2.4
Or see this region's nucleotide sequence