Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT1233

Experiment: Varel_Bryant_medium_Glucose with Lincomycin 0.0015625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT1231 and tRNA-Ser are separated by 95 nucleotidestRNA-Ser and tRNA-Glu are separated by 63 nucleotidestRNA-Glu and BT1232 are separated by 67 nucleotidesBT1232 and BT1233 are separated by 169 nucleotidesBT1233 and BT1234 are separated by 100 nucleotidesBT1234 and BT1235 are separated by 17 nucleotides BT1231: BT1231 - hypothetical protein (NCBI ptt file), at 1,533,844 to 1,534,047 BT1231 BT_t21: tRNA-Ser - tRNA-Ser (RefSeq), at 1,534,143 to 1,534,232 tRNA-Ser BT_t22: tRNA-Glu - tRNA-Glu (RefSeq), at 1,534,296 to 1,534,367 tRNA-Glu BT1232: BT1232 - hypothetical protein (NCBI ptt file), at 1,534,435 to 1,534,641 BT1232 BT1233: BT1233 - hypothetical protein (NCBI ptt file), at 1,534,811 to 1,535,545 BT1233 BT1234: BT1234 - conserved hypothetical protein (NCBI ptt file), at 1,535,646 to 1,536,230 BT1234 BT1235: BT1235 - AraC-type transcription regulator (NCBI ptt file), at 1,536,248 to 1,537,150 BT1235 Position (kb) 1534 1535 1536Strain fitness (log2 ratio) -3 -2 -1 0 1at 1533.862 kb on - strandat 1533.912 kb on + strand, within BT1231at 1534.036 kb on + strandat 1534.339 kb on + strand, within tRNA-Gluat 1534.340 kb on - strand, within tRNA-Gluat 1534.373 kb on - strandat 1534.377 kb on + strandat 1534.494 kb on + strand, within BT1232at 1534.593 kb on + strand, within BT1232at 1534.619 kb on - strand, within BT1232at 1534.745 kb on + strandat 1534.809 kb on - strandat 1534.812 kb on + strandat 1534.921 kb on - strand, within BT1233at 1534.923 kb on - strand, within BT1233at 1535.177 kb on - strand, within BT1233at 1535.182 kb on + strand, within BT1233at 1535.206 kb on - strand, within BT1233at 1535.219 kb on - strand, within BT1233at 1535.248 kb on + strand, within BT1233at 1535.352 kb on - strand, within BT1233at 1535.399 kb on + strand, within BT1233at 1535.399 kb on + strand, within BT1233at 1535.400 kb on - strand, within BT1233at 1535.466 kb on + strand, within BT1233at 1535.467 kb on - strand, within BT1233at 1535.468 kb on + strand, within BT1233at 1535.545 kb on + strandat 1535.546 kb on - strandat 1535.547 kb on + strandat 1535.573 kb on - strandat 1535.573 kb on - strandat 1535.614 kb on - strandat 1535.651 kb on + strandat 1535.708 kb on + strand, within BT1234at 1535.728 kb on + strand, within BT1234at 1535.836 kb on - strand, within BT1234at 1535.968 kb on - strand, within BT1234at 1536.228 kb on + strandat 1536.228 kb on + strandat 1536.237 kb on - strandat 1536.250 kb on - strandat 1536.280 kb on + strandat 1536.295 kb on + strandat 1536.295 kb on + strandat 1536.296 kb on - strandat 1536.300 kb on + strandat 1536.305 kb on - strandat 1536.345 kb on - strand, within BT1235at 1536.520 kb on - strand, within BT1235

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Per-strain Table

Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Lincomycin 0.0015625 mM
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1,533,862 - -2.5
1,533,912 + BT1231 0.33 +0.1
1,534,036 + -1.7
1,534,339 + tRNA-Glu BT_t22 0.60 +1.1
1,534,340 - tRNA-Glu BT_t22 0.61 +0.1
1,534,373 - +0.1
1,534,377 + +0.6
1,534,494 + BT1232 0.29 -1.9
1,534,593 + BT1232 0.76 -1.0
1,534,619 - BT1232 0.89 +0.1
1,534,745 + -0.3
1,534,809 - -1.7
1,534,812 + -1.9
1,534,921 - BT1233 0.15 -0.9
1,534,923 - BT1233 0.15 +1.1
1,535,177 - BT1233 0.50 -1.1
1,535,182 + BT1233 0.50 +0.5
1,535,206 - BT1233 0.54 -1.5
1,535,219 - BT1233 0.56 +0.8
1,535,248 + BT1233 0.59 +0.4
1,535,352 - BT1233 0.74 +0.1
1,535,399 + BT1233 0.80 -0.7
1,535,399 + BT1233 0.80 -2.2
1,535,400 - BT1233 0.80 +0.1
1,535,466 + BT1233 0.89 +0.1
1,535,467 - BT1233 0.89 -0.9
1,535,468 + BT1233 0.89 -2.2
1,535,545 + +1.1
1,535,546 - -0.1
1,535,547 + -1.5
1,535,573 - -0.9
1,535,573 - +0.1
1,535,614 - -2.9
1,535,651 + -2.5
1,535,708 + BT1234 0.11 -2.2
1,535,728 + BT1234 0.14 -1.5
1,535,836 - BT1234 0.32 -0.9
1,535,968 - BT1234 0.55 -2.1
1,536,228 + -1.5
1,536,228 + -0.4
1,536,237 - -0.9
1,536,250 - +0.1
1,536,280 + +0.7
1,536,295 + +0.1
1,536,295 + -0.5
1,536,296 - -2.4
1,536,300 + -0.9
1,536,305 - -1.6
1,536,345 - BT1235 0.11 -1.1
1,536,520 - BT1235 0.30 +1.1

Or see this region's nucleotide sequence