Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04074 and H281DRAFT_04075 overlap by 4 nucleotides H281DRAFT_04075 and H281DRAFT_04076 are separated by 158 nucleotides
H281DRAFT_04074: H281DRAFT_04074 - 16S rRNA (cytosine967-C5)-methyltransferase, at 176,101 to 177,360
_04074
H281DRAFT_04075: H281DRAFT_04075 - formyltetrahydrofolate-dependent phosphoribosylglycinamide formyltransferase, at 177,357 to 177,968
_04075
H281DRAFT_04076: H281DRAFT_04076 - FMN adenylyltransferase /riboflavin kinase, at 178,127 to 179,122
_04076
Position (kb)
177
178 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 176.606 kb on + strand, within H281DRAFT_04074 at 176.606 kb on + strand, within H281DRAFT_04074 at 176.607 kb on - strand, within H281DRAFT_04074 at 176.607 kb on - strand, within H281DRAFT_04074 at 176.607 kb on - strand, within H281DRAFT_04074 at 176.866 kb on + strand, within H281DRAFT_04074 at 176.866 kb on + strand, within H281DRAFT_04074 at 176.866 kb on + strand, within H281DRAFT_04074 at 176.866 kb on + strand, within H281DRAFT_04074 at 176.867 kb on - strand, within H281DRAFT_04074 at 176.921 kb on + strand, within H281DRAFT_04074 at 176.984 kb on + strand, within H281DRAFT_04074 at 176.985 kb on - strand, within H281DRAFT_04074 at 176.985 kb on - strand, within H281DRAFT_04074 at 176.985 kb on - strand, within H281DRAFT_04074 at 176.985 kb on - strand, within H281DRAFT_04074 at 177.352 kb on + strand at 177.352 kb on + strand at 177.352 kb on + strand at 177.352 kb on + strand at 177.352 kb on + strand at 177.352 kb on + strand at 177.352 kb on + strand at 177.352 kb on + strand at 177.352 kb on + strand at 177.353 kb on - strand at 177.413 kb on + strand at 177.414 kb on - strand at 177.414 kb on - strand at 177.479 kb on - strand, within H281DRAFT_04075 at 177.482 kb on + strand, within H281DRAFT_04075 at 177.482 kb on + strand, within H281DRAFT_04075 at 177.482 kb on + strand, within H281DRAFT_04075 at 177.482 kb on + strand, within H281DRAFT_04075 at 177.482 kb on + strand, within H281DRAFT_04075 at 177.482 kb on + strand, within H281DRAFT_04075 at 177.482 kb on + strand, within H281DRAFT_04075 at 177.482 kb on + strand, within H281DRAFT_04075 at 177.482 kb on + strand, within H281DRAFT_04075 at 177.482 kb on + strand, within H281DRAFT_04075 at 177.483 kb on - strand, within H281DRAFT_04075 at 177.483 kb on - strand, within H281DRAFT_04075 at 177.483 kb on - strand, within H281DRAFT_04075 at 177.744 kb on - strand, within H281DRAFT_04075 at 177.959 kb on + strand at 177.959 kb on + strand at 177.960 kb on - strand at 178.076 kb on + strand at 178.076 kb on + strand at 178.077 kb on - strand at 178.078 kb on + strand at 178.078 kb on + strand at 178.078 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 176,606 + H281DRAFT_04074 0.40 +0.6 176,606 + H281DRAFT_04074 0.40 -1.2 176,607 - H281DRAFT_04074 0.40 -0.3 176,607 - H281DRAFT_04074 0.40 +0.5 176,607 - H281DRAFT_04074 0.40 +0.2 176,866 + H281DRAFT_04074 0.61 -0.2 176,866 + H281DRAFT_04074 0.61 -0.0 176,866 + H281DRAFT_04074 0.61 +2.4 176,866 + H281DRAFT_04074 0.61 -0.2 176,867 - H281DRAFT_04074 0.61 -0.1 176,921 + H281DRAFT_04074 0.65 -2.2 176,984 + H281DRAFT_04074 0.70 +0.1 176,985 - H281DRAFT_04074 0.70 +0.9 176,985 - H281DRAFT_04074 0.70 +1.6 176,985 - H281DRAFT_04074 0.70 +0.1 176,985 - H281DRAFT_04074 0.70 -0.3 177,352 + -0.5 177,352 + -0.2 177,352 + -1.0 177,352 + +1.9 177,352 + -1.5 177,352 + +0.6 177,352 + -0.5 177,352 + +0.3 177,352 + -0.4 177,353 - -0.1 177,413 + -1.2 177,414 - -0.4 177,414 - +0.6 177,479 - H281DRAFT_04075 0.20 -1.5 177,482 + H281DRAFT_04075 0.20 +0.3 177,482 + H281DRAFT_04075 0.20 -0.6 177,482 + H281DRAFT_04075 0.20 -0.7 177,482 + H281DRAFT_04075 0.20 -0.7 177,482 + H281DRAFT_04075 0.20 -0.9 177,482 + H281DRAFT_04075 0.20 +0.2 177,482 + H281DRAFT_04075 0.20 +0.1 177,482 + H281DRAFT_04075 0.20 +0.6 177,482 + H281DRAFT_04075 0.20 -3.0 177,482 + H281DRAFT_04075 0.20 +0.1 177,483 - H281DRAFT_04075 0.21 -0.1 177,483 - H281DRAFT_04075 0.21 +0.4 177,483 - H281DRAFT_04075 0.21 +1.6 177,744 - H281DRAFT_04075 0.63 +0.1 177,959 + -0.9 177,959 + +1.2 177,960 - +0.2 178,076 + +0.6 178,076 + +0.5 178,077 - -0.4 178,078 + +0.1 178,078 + -0.2 178,078 + -0.5
Or see this region's nucleotide sequence