Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_02815 and H281DRAFT_02816 overlap by 4 nucleotides H281DRAFT_02816 and H281DRAFT_02817 are separated by 207 nucleotides H281DRAFT_02817 and H281DRAFT_02818 are separated by 284 nucleotides H281DRAFT_02818 and H281DRAFT_02819 are separated by 220 nucleotides
H281DRAFT_02815: H281DRAFT_02815 - xanthine dehydrogenase YagS FAD-binding subunit, at 406,469 to 407,473
_02815
H281DRAFT_02816: H281DRAFT_02816 - xanthine dehydrogenase YagT iron-sulfur-binding subunit, at 407,470 to 408,012
_02816
H281DRAFT_02817: H281DRAFT_02817 - hypothetical protein, at 408,220 to 409,086
_02817
H281DRAFT_02818: H281DRAFT_02818 - Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain, at 409,371 to 409,850
_02818
H281DRAFT_02819: H281DRAFT_02819 - DNA ligase-1, at 410,071 to 411,756
_02819
Position (kb) 408
409
410 Strain fitness (log2 ratio) -1
0
1 at 407.544 kb on - strand, within H281DRAFT_02816 at 408.221 kb on + strand at 408.270 kb on - strand at 408.270 kb on - strand at 408.677 kb on + strand, within H281DRAFT_02817 at 408.678 kb on - strand, within H281DRAFT_02817 at 408.689 kb on + strand, within H281DRAFT_02817 at 408.689 kb on + strand, within H281DRAFT_02817 at 409.089 kb on - strand at 409.530 kb on - strand, within H281DRAFT_02818 at 409.854 kb on + strand at 409.854 kb on + strand at 409.854 kb on + strand at 410.050 kb on - strand at 410.050 kb on - strand at 410.053 kb on + strand at 410.054 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 407,544 - H281DRAFT_02816 0.14 -0.7 408,221 + -1.0 408,270 - -0.5 408,270 - +0.3 408,677 + H281DRAFT_02817 0.53 +0.6 408,678 - H281DRAFT_02817 0.53 -0.1 408,689 + H281DRAFT_02817 0.54 +0.5 408,689 + H281DRAFT_02817 0.54 +0.2 409,089 - -1.1 409,530 - H281DRAFT_02818 0.33 -0.2 409,854 + +0.8 409,854 + -1.0 409,854 + -0.1 410,050 - +0.3 410,050 - -0.8 410,053 + +0.4 410,054 - +0.3
Or see this region's nucleotide sequence