Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_00585 and H281DRAFT_00586 overlap by 4 nucleotides H281DRAFT_00586 and H281DRAFT_00587 are separated by 10 nucleotides H281DRAFT_00587 and H281DRAFT_00588 are separated by 61 nucleotides
H281DRAFT_00585: H281DRAFT_00585 - DNA polymerase, at 651,693 to 652,901
_00585
H281DRAFT_00586: H281DRAFT_00586 - hypothetical protein, at 652,898 to 653,995
_00586
H281DRAFT_00587: H281DRAFT_00587 - hydroxymethylpyrimidine kinase /phosphomethylpyrimidine kinase, at 654,006 to 654,815
_00587
H281DRAFT_00588: H281DRAFT_00588 - Major Facilitator Superfamily protein, at 654,877 to 656,184
_00588
Position (kb) 652
653
654 Strain fitness (log2 ratio) -1
0
1 at 652.854 kb on - strand at 653.119 kb on + strand, within H281DRAFT_00586 at 653.356 kb on - strand, within H281DRAFT_00586 at 653.356 kb on - strand, within H281DRAFT_00586 at 653.405 kb on + strand, within H281DRAFT_00586 at 653.489 kb on + strand, within H281DRAFT_00586 at 653.489 kb on + strand, within H281DRAFT_00586 at 653.489 kb on + strand, within H281DRAFT_00586 at 653.489 kb on + strand, within H281DRAFT_00586 at 653.490 kb on - strand, within H281DRAFT_00586 at 653.639 kb on + strand, within H281DRAFT_00586 at 653.795 kb on + strand, within H281DRAFT_00586 at 653.796 kb on - strand, within H281DRAFT_00586 at 653.796 kb on - strand, within H281DRAFT_00586 at 653.884 kb on + strand, within H281DRAFT_00586 at 654.019 kb on + strand at 654.019 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 652,854 - -0.9 653,119 + H281DRAFT_00586 0.20 +0.3 653,356 - H281DRAFT_00586 0.42 -0.0 653,356 - H281DRAFT_00586 0.42 -1.1 653,405 + H281DRAFT_00586 0.46 +0.4 653,489 + H281DRAFT_00586 0.54 +0.1 653,489 + H281DRAFT_00586 0.54 -0.7 653,489 + H281DRAFT_00586 0.54 +0.7 653,489 + H281DRAFT_00586 0.54 +0.3 653,490 - H281DRAFT_00586 0.54 -0.6 653,639 + H281DRAFT_00586 0.67 +0.5 653,795 + H281DRAFT_00586 0.82 -0.1 653,796 - H281DRAFT_00586 0.82 -0.0 653,796 - H281DRAFT_00586 0.82 -0.8 653,884 + H281DRAFT_00586 0.90 +0.3 654,019 + -0.5 654,019 + -0.0
Or see this region's nucleotide sequence