Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_03118 and H281DRAFT_03119 overlap by 4 nucleotides H281DRAFT_03119 and H281DRAFT_03120 are separated by 23 nucleotides H281DRAFT_03120 and H281DRAFT_03121 are separated by 27 nucleotides H281DRAFT_03121 and H281DRAFT_03122 overlap by 8 nucleotides
H281DRAFT_03118: H281DRAFT_03118 - NADPH-dependent 2,4-dienoyl-CoA reductase, sulfur reductase, at 151,204 to 152,637
_03118
H281DRAFT_03119: H281DRAFT_03119 - NADH dehydrogenase/NADH:ubiquinone oxidoreductase 75 kD subunit (chain G), at 152,634 to 153,002
_03119
H281DRAFT_03120: H281DRAFT_03120 - putative spermidine/putrescine transport system permease protein, at 153,026 to 153,820
_03120
H281DRAFT_03121: H281DRAFT_03121 - putative spermidine/putrescine transport system permease protein, at 153,848 to 154,762
_03121
H281DRAFT_03122: H281DRAFT_03122 - putative spermidine/putrescine transport system ATP-binding protein, at 154,755 to 155,846
_03122
Position (kb) 153
154 Strain fitness (log2 ratio) -1
0
1 at 152.230 kb on - strand, within H281DRAFT_03118 at 153.158 kb on - strand, within H281DRAFT_03120 at 153.158 kb on - strand, within H281DRAFT_03120 at 153.160 kb on - strand, within H281DRAFT_03120 at 153.397 kb on + strand, within H281DRAFT_03120 at 153.867 kb on + strand at 153.867 kb on + strand at 153.891 kb on + strand at 154.260 kb on + strand at 154.435 kb on - strand at 154.519 kb on - strand, within H281DRAFT_03121
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 152,230 - H281DRAFT_03118 0.72 +0.8 153,158 - H281DRAFT_03120 0.17 -0.1 153,158 - H281DRAFT_03120 0.17 -0.2 153,160 - H281DRAFT_03120 0.17 -0.9 153,397 + H281DRAFT_03120 0.47 +1.1 153,867 + -0.1 153,867 + -0.1 153,891 + -0.2 154,260 + -0.2 154,435 - -0.6 154,519 - H281DRAFT_03121 0.73 +0.2
Or see this region's nucleotide sequence