Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_03117 and H281DRAFT_03118 overlap by 8 nucleotides H281DRAFT_03118 and H281DRAFT_03119 overlap by 4 nucleotides H281DRAFT_03119 and H281DRAFT_03120 are separated by 23 nucleotides
H281DRAFT_03117: H281DRAFT_03117 - sarcosine oxidase subunit beta, at 150,084 to 151,211
_03117
H281DRAFT_03118: H281DRAFT_03118 - NADPH-dependent 2,4-dienoyl-CoA reductase, sulfur reductase, at 151,204 to 152,637
_03118
H281DRAFT_03119: H281DRAFT_03119 - NADH dehydrogenase/NADH:ubiquinone oxidoreductase 75 kD subunit (chain G), at 152,634 to 153,002
_03119
H281DRAFT_03120: H281DRAFT_03120 - putative spermidine/putrescine transport system permease protein, at 153,026 to 153,820
_03120
Position (kb) 151
152
153 Strain fitness (log2 ratio) -1
0
1 at 150.656 kb on + strand, within H281DRAFT_03117 at 151.056 kb on + strand, within H281DRAFT_03117 at 151.250 kb on + strand at 151.251 kb on - strand at 151.251 kb on - strand at 151.456 kb on + strand, within H281DRAFT_03118 at 152.009 kb on + strand, within H281DRAFT_03118 at 152.230 kb on - strand, within H281DRAFT_03118 at 153.158 kb on - strand, within H281DRAFT_03120 at 153.158 kb on - strand, within H281DRAFT_03120 at 153.160 kb on - strand, within H281DRAFT_03120 at 153.397 kb on + strand, within H281DRAFT_03120
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 150,656 + H281DRAFT_03117 0.51 +0.4 151,056 + H281DRAFT_03117 0.86 -0.1 151,250 + -0.3 151,251 - +0.8 151,251 - -0.5 151,456 + H281DRAFT_03118 0.18 +1.5 152,009 + H281DRAFT_03118 0.56 +0.6 152,230 - H281DRAFT_03118 0.72 +0.8 153,158 - H281DRAFT_03120 0.17 -0.1 153,158 - H281DRAFT_03120 0.17 -0.2 153,160 - H281DRAFT_03120 0.17 -0.9 153,397 + H281DRAFT_03120 0.47 +1.1
Or see this region's nucleotide sequence