Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_03116 and H281DRAFT_03117 are separated by 29 nucleotides H281DRAFT_03117 and H281DRAFT_03118 overlap by 8 nucleotides
H281DRAFT_03116: H281DRAFT_03116 - putative spermidine/putrescine transport system substrate-binding protein, at 149,008 to 150,054
_03116
H281DRAFT_03117: H281DRAFT_03117 - sarcosine oxidase subunit beta, at 150,084 to 151,211
_03117
H281DRAFT_03118: H281DRAFT_03118 - NADPH-dependent 2,4-dienoyl-CoA reductase, sulfur reductase, at 151,204 to 152,637
_03118
Position (kb) 150
151
152 Strain fitness (log2 ratio) -1
0
1 at 149.313 kb on - strand, within H281DRAFT_03116 at 149.426 kb on + strand, within H281DRAFT_03116 at 149.427 kb on - strand, within H281DRAFT_03116 at 149.652 kb on - strand, within H281DRAFT_03116 at 149.652 kb on - strand, within H281DRAFT_03116 at 149.652 kb on - strand, within H281DRAFT_03116 at 149.854 kb on - strand, within H281DRAFT_03116 at 150.102 kb on - strand at 150.656 kb on + strand, within H281DRAFT_03117 at 151.056 kb on + strand, within H281DRAFT_03117 at 151.250 kb on + strand at 151.251 kb on - strand at 151.251 kb on - strand at 151.456 kb on + strand, within H281DRAFT_03118 at 152.009 kb on + strand, within H281DRAFT_03118
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 149,313 - H281DRAFT_03116 0.29 -0.8 149,426 + H281DRAFT_03116 0.40 +0.4 149,427 - H281DRAFT_03116 0.40 +0.2 149,652 - H281DRAFT_03116 0.62 -0.3 149,652 - H281DRAFT_03116 0.62 -0.3 149,652 - H281DRAFT_03116 0.62 -1.2 149,854 - H281DRAFT_03116 0.81 +0.6 150,102 - +0.2 150,656 + H281DRAFT_03117 0.51 +0.4 151,056 + H281DRAFT_03117 0.86 -0.1 151,250 + -0.3 151,251 - +0.8 151,251 - -0.5 151,456 + H281DRAFT_03118 0.18 +1.5 152,009 + H281DRAFT_03118 0.56 +0.6
Or see this region's nucleotide sequence