Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_02358 and H281DRAFT_02359 are separated by 139 nucleotides H281DRAFT_02359 and H281DRAFT_02360 are separated by 413 nucleotides
H281DRAFT_02358: H281DRAFT_02358 - GTPase, G3E family, at 517,084 to 518,400
_02358
H281DRAFT_02359: H281DRAFT_02359 - HU family DNA-binding protein, at 518,540 to 518,818
_02359
H281DRAFT_02360: H281DRAFT_02360 - tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein, at 519,232 to 521,211
_02360
Position (kb)
518
519 Strain fitness (log2 ratio)
-1
0
1
2 at 517.848 kb on + strand, within H281DRAFT_02358 at 517.848 kb on + strand, within H281DRAFT_02358 at 517.849 kb on - strand, within H281DRAFT_02358 at 517.849 kb on - strand, within H281DRAFT_02358 at 518.268 kb on - strand, within H281DRAFT_02358 at 518.412 kb on + strand at 518.413 kb on - strand at 518.416 kb on - strand at 518.533 kb on - strand at 518.819 kb on + strand at 518.820 kb on - strand at 518.838 kb on + strand at 518.838 kb on + strand at 518.839 kb on - strand at 518.901 kb on + strand at 518.901 kb on + strand at 518.985 kb on + strand at 518.985 kb on + strand at 518.985 kb on + strand at 518.986 kb on - strand at 519.051 kb on + strand at 519.052 kb on - strand at 519.068 kb on - strand at 519.245 kb on + strand at 519.299 kb on - strand at 519.306 kb on + strand at 519.306 kb on + strand at 519.306 kb on + strand at 519.307 kb on - strand at 519.307 kb on - strand at 519.310 kb on + strand at 519.310 kb on + strand at 519.310 kb on + strand at 519.310 kb on + strand at 519.311 kb on - strand at 519.311 kb on - strand at 519.311 kb on - strand at 519.357 kb on - strand at 519.357 kb on - strand at 519.357 kb on - strand at 519.547 kb on + strand, within H281DRAFT_02360 at 519.659 kb on + strand, within H281DRAFT_02360 at 519.659 kb on + strand, within H281DRAFT_02360 at 519.659 kb on + strand, within H281DRAFT_02360 at 519.660 kb on - strand, within H281DRAFT_02360 at 519.671 kb on + strand, within H281DRAFT_02360 at 519.671 kb on + strand, within H281DRAFT_02360 at 519.671 kb on + strand, within H281DRAFT_02360 at 519.671 kb on + strand, within H281DRAFT_02360 at 519.672 kb on - strand, within H281DRAFT_02360 at 519.731 kb on - strand, within H281DRAFT_02360 at 519.731 kb on - strand, within H281DRAFT_02360
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 517,848 + H281DRAFT_02358 0.58 +0.2 517,848 + H281DRAFT_02358 0.58 -0.6 517,849 - H281DRAFT_02358 0.58 -1.1 517,849 - H281DRAFT_02358 0.58 -0.2 518,268 - H281DRAFT_02358 0.90 -1.3 518,412 + +0.3 518,413 - +0.5 518,416 - +0.1 518,533 - +0.4 518,819 + -0.0 518,820 - -0.0 518,838 + +0.9 518,838 + -0.3 518,839 - -0.1 518,901 + +0.9 518,901 + -0.1 518,985 + +0.9 518,985 + -1.0 518,985 + +0.6 518,986 - +2.0 519,051 + +1.0 519,052 - +0.6 519,068 - +0.6 519,245 + +0.1 519,299 - +0.3 519,306 + -0.9 519,306 + -0.8 519,306 + +0.6 519,307 - +0.2 519,307 - -1.4 519,310 + -0.2 519,310 + +0.3 519,310 + -0.6 519,310 + -0.6 519,311 - +0.2 519,311 - +1.7 519,311 - +1.0 519,357 - -0.7 519,357 - -0.1 519,357 - -0.0 519,547 + H281DRAFT_02360 0.16 -0.7 519,659 + H281DRAFT_02360 0.22 +0.2 519,659 + H281DRAFT_02360 0.22 +0.4 519,659 + H281DRAFT_02360 0.22 +0.4 519,660 - H281DRAFT_02360 0.22 -0.9 519,671 + H281DRAFT_02360 0.22 +0.1 519,671 + H281DRAFT_02360 0.22 +0.1 519,671 + H281DRAFT_02360 0.22 +0.5 519,671 + H281DRAFT_02360 0.22 +0.3 519,672 - H281DRAFT_02360 0.22 +0.6 519,731 - H281DRAFT_02360 0.25 -0.5 519,731 - H281DRAFT_02360 0.25 +0.4
Or see this region's nucleotide sequence