Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_01321

Experiment: LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_01319 and H281DRAFT_01320 are separated by 93 nucleotidesH281DRAFT_01320 and H281DRAFT_01321 are separated by 103 nucleotidesH281DRAFT_01321 and H281DRAFT_01322 overlap by 4 nucleotides H281DRAFT_01319: H281DRAFT_01319 - Uncharacterized protein, UPF0264 family, at 159,857 to 160,600 _01319 H281DRAFT_01320: H281DRAFT_01320 - Uncharacterized archaeal kinase related to aspartokinases, uridylate kinases, at 160,694 to 161,284 _01320 H281DRAFT_01321: H281DRAFT_01321 - probable H4MPT-linked C1 transfer pathway protein, at 161,388 to 162,512 _01321 H281DRAFT_01322: H281DRAFT_01322 - Predicted ATP-dependent carboligase, ATP-grasp superfamily, at 162,509 to 163,642 _01322 Position (kb) 161 162 163Strain fitness (log2 ratio) -1 0 1 2at 160.598 kb on + strandat 160.599 kb on - strandat 160.634 kb on - strandat 160.747 kb on + strandat 160.747 kb on + strandat 160.767 kb on + strand, within H281DRAFT_01320at 161.819 kb on - strand, within H281DRAFT_01321at 162.184 kb on + strand, within H281DRAFT_01321at 162.724 kb on - strand, within H281DRAFT_01322

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Per-strain Table

Position Strand Gene LocusTag Fraction LB
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160,598 + -0.5
160,599 - +0.7
160,634 - -0.6
160,747 + +2.4
160,747 + -0.2
160,767 + H281DRAFT_01320 0.12 -0.3
161,819 - H281DRAFT_01321 0.38 -1.4
162,184 + H281DRAFT_01321 0.71 -0.5
162,724 - H281DRAFT_01322 0.19 -0.8

Or see this region's nucleotide sequence