Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_00279 and H281DRAFT_00280 overlap by 7 nucleotides H281DRAFT_00280 and H281DRAFT_00281 overlap by 4 nucleotides H281DRAFT_00281 and H281DRAFT_00282 are separated by 443 nucleotides H281DRAFT_00282 and H281DRAFT_00283 overlap by 1 nucleotides
H281DRAFT_00279: H281DRAFT_00279 - chorismate lyase, at 300,185 to 300,901
_00279
H281DRAFT_00280: H281DRAFT_00280 - G/U mismatch-specific uracil-DNA glycosylase, at 300,895 to 301,368
_00280
H281DRAFT_00281: H281DRAFT_00281 - Spermidine synthase, at 301,365 to 302,276
_00281
H281DRAFT_00282: H281DRAFT_00282 - Protein of unknown function (DUF3311), at 302,720 to 302,959
_00282
H281DRAFT_00283: H281DRAFT_00283 - solute:Na+ symporter, SSS family, at 302,959 to 304,509
_00283
Position (kb) 301
302
303 Strain fitness (log2 ratio) -1
0
1
2 at 300.487 kb on - strand, within H281DRAFT_00279 at 300.537 kb on - strand, within H281DRAFT_00279 at 300.537 kb on - strand, within H281DRAFT_00279 at 300.603 kb on - strand, within H281DRAFT_00279 at 300.742 kb on - strand, within H281DRAFT_00279 at 300.750 kb on + strand, within H281DRAFT_00279 at 300.751 kb on - strand, within H281DRAFT_00279 at 300.936 kb on + strand at 300.936 kb on + strand at 300.936 kb on + strand at 300.937 kb on - strand at 300.991 kb on + strand, within H281DRAFT_00280 at 301.289 kb on + strand, within H281DRAFT_00280 at 301.289 kb on + strand, within H281DRAFT_00280 at 301.289 kb on + strand, within H281DRAFT_00280 at 301.290 kb on - strand, within H281DRAFT_00280 at 301.317 kb on + strand, within H281DRAFT_00280 at 301.317 kb on + strand, within H281DRAFT_00280 at 301.317 kb on + strand, within H281DRAFT_00280 at 301.317 kb on + strand, within H281DRAFT_00280 at 301.318 kb on - strand, within H281DRAFT_00280 at 301.567 kb on - strand, within H281DRAFT_00281 at 301.683 kb on - strand, within H281DRAFT_00281 at 301.921 kb on - strand, within H281DRAFT_00281 at 302.364 kb on - strand at 302.574 kb on + strand at 302.575 kb on - strand at 302.575 kb on - strand at 302.575 kb on - strand at 302.576 kb on + strand at 302.576 kb on + strand at 302.576 kb on + strand at 302.577 kb on - strand at 302.700 kb on - strand at 302.700 kb on - strand at 302.702 kb on + strand at 302.852 kb on + strand, within H281DRAFT_00282 at 302.907 kb on - strand, within H281DRAFT_00282 at 302.908 kb on + strand, within H281DRAFT_00282 at 302.909 kb on - strand, within H281DRAFT_00282 at 302.909 kb on - strand, within H281DRAFT_00282 at 302.990 kb on + strand at 303.140 kb on - strand, within H281DRAFT_00283 at 303.206 kb on - strand, within H281DRAFT_00283
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 300,487 - H281DRAFT_00279 0.42 +0.5 300,537 - H281DRAFT_00279 0.49 +0.6 300,537 - H281DRAFT_00279 0.49 +0.4 300,603 - H281DRAFT_00279 0.58 -0.4 300,742 - H281DRAFT_00279 0.78 -0.9 300,750 + H281DRAFT_00279 0.79 -0.2 300,751 - H281DRAFT_00279 0.79 +0.1 300,936 + -0.9 300,936 + +0.3 300,936 + -0.1 300,937 - +0.7 300,991 + H281DRAFT_00280 0.20 -0.0 301,289 + H281DRAFT_00280 0.83 -0.3 301,289 + H281DRAFT_00280 0.83 +0.1 301,289 + H281DRAFT_00280 0.83 +1.3 301,290 - H281DRAFT_00280 0.83 +0.1 301,317 + H281DRAFT_00280 0.89 +0.2 301,317 + H281DRAFT_00280 0.89 +1.0 301,317 + H281DRAFT_00280 0.89 +1.5 301,317 + H281DRAFT_00280 0.89 +0.1 301,318 - H281DRAFT_00280 0.89 -0.2 301,567 - H281DRAFT_00281 0.22 +0.9 301,683 - H281DRAFT_00281 0.35 +0.1 301,921 - H281DRAFT_00281 0.61 +1.0 302,364 - +0.3 302,574 + -0.0 302,575 - +1.4 302,575 - -0.3 302,575 - +0.2 302,576 + -0.1 302,576 + -0.5 302,576 + +0.7 302,577 - +0.4 302,700 - +2.5 302,700 - +0.1 302,702 + +0.1 302,852 + H281DRAFT_00282 0.55 -1.4 302,907 - H281DRAFT_00282 0.78 -0.0 302,908 + H281DRAFT_00282 0.78 +1.5 302,909 - H281DRAFT_00282 0.79 -0.3 302,909 - H281DRAFT_00282 0.79 +0.3 302,990 + -0.1 303,140 - H281DRAFT_00283 0.12 -0.2 303,206 - H281DRAFT_00283 0.16 -0.9
Or see this region's nucleotide sequence