Experiment: Varel_Bryant_medium_Glucose with Doxycycline hyclate 0.00009765625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0451 and BT0452 are separated by 18 nucleotides BT0452 and BT0453 are separated by 54 nucleotides
BT0451: BT0451 - putative outer membrane protein, probably involved in nutrient binding (NCBI ptt file), at 550,603 to 552,264
BT0451
BT0452: BT0452 - putative outer membrane protein, probably involved in nutrient binding (NCBI ptt file), at 552,283 to 555,507
BT0452
BT0453: BT0453 - N-acylglucosamine 2-epimerase (NCBI ptt file), at 555,562 to 556,725
BT0453
Position (kb) 552
553
554
555
556 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 551.316 kb on - strand, within BT0451 at 551.330 kb on - strand, within BT0451 at 551.373 kb on + strand, within BT0451 at 551.520 kb on + strand, within BT0451 at 551.521 kb on - strand, within BT0451 at 551.606 kb on + strand, within BT0451 at 551.737 kb on - strand, within BT0451 at 551.758 kb on + strand, within BT0451 at 551.774 kb on + strand, within BT0451 at 551.793 kb on + strand, within BT0451 at 551.795 kb on + strand, within BT0451 at 551.797 kb on + strand, within BT0451 at 551.797 kb on + strand, within BT0451 at 551.797 kb on + strand, within BT0451 at 551.912 kb on + strand, within BT0451 at 552.003 kb on + strand, within BT0451 at 552.104 kb on + strand at 552.197 kb on + strand at 552.204 kb on + strand at 552.205 kb on - strand at 552.205 kb on - strand at 552.264 kb on + strand at 552.332 kb on + strand at 552.332 kb on + strand at 552.409 kb on + strand at 552.465 kb on + strand at 552.465 kb on + strand at 552.465 kb on + strand at 552.529 kb on + strand at 552.604 kb on - strand at 552.623 kb on + strand, within BT0452 at 552.626 kb on - strand, within BT0452 at 552.785 kb on + strand, within BT0452 at 552.955 kb on + strand, within BT0452 at 552.970 kb on + strand, within BT0452 at 553.160 kb on + strand, within BT0452 at 553.165 kb on - strand, within BT0452 at 553.169 kb on + strand at 553.223 kb on + strand, within BT0452 at 553.224 kb on - strand, within BT0452 at 553.229 kb on + strand, within BT0452 at 553.230 kb on - strand, within BT0452 at 553.236 kb on + strand, within BT0452 at 553.298 kb on + strand, within BT0452 at 553.299 kb on - strand, within BT0452 at 553.418 kb on + strand, within BT0452 at 553.423 kb on - strand, within BT0452 at 553.582 kb on + strand, within BT0452 at 553.641 kb on + strand, within BT0452 at 553.650 kb on - strand, within BT0452 at 553.683 kb on + strand, within BT0452 at 553.819 kb on + strand, within BT0452 at 553.819 kb on + strand, within BT0452 at 553.989 kb on + strand, within BT0452 at 554.065 kb on + strand, within BT0452 at 554.094 kb on + strand, within BT0452 at 554.094 kb on + strand, within BT0452 at 554.096 kb on + strand, within BT0452 at 554.097 kb on - strand, within BT0452 at 554.203 kb on - strand, within BT0452 at 554.256 kb on + strand, within BT0452 at 554.314 kb on - strand, within BT0452 at 554.374 kb on + strand, within BT0452 at 554.423 kb on + strand, within BT0452 at 554.433 kb on - strand, within BT0452 at 554.451 kb on + strand, within BT0452 at 554.544 kb on + strand, within BT0452 at 554.565 kb on - strand, within BT0452 at 554.689 kb on - strand, within BT0452 at 554.700 kb on + strand, within BT0452 at 554.764 kb on + strand, within BT0452 at 554.775 kb on + strand, within BT0452 at 554.852 kb on + strand, within BT0452 at 554.887 kb on + strand, within BT0452 at 554.904 kb on + strand, within BT0452 at 554.948 kb on + strand, within BT0452 at 554.949 kb on - strand, within BT0452 at 555.247 kb on + strand at 555.247 kb on + strand at 555.248 kb on - strand at 555.267 kb on + strand at 555.319 kb on - strand at 555.319 kb on - strand at 555.327 kb on + strand at 555.595 kb on + strand at 555.793 kb on + strand, within BT0453 at 556.194 kb on + strand, within BT0453 at 556.343 kb on - strand, within BT0453 at 556.444 kb on - strand, within BT0453
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Doxycycline hyclate 0.00009765625 mM remove 551,316 - BT0451 0.43 -0.1 551,330 - BT0451 0.44 -1.5 551,373 + BT0451 0.46 -1.1 551,520 + BT0451 0.55 +0.9 551,521 - BT0451 0.55 -2.9 551,606 + BT0451 0.60 -1.3 551,737 - BT0451 0.68 +0.3 551,758 + BT0451 0.69 +0.2 551,774 + BT0451 0.70 -0.4 551,793 + BT0451 0.72 +1.9 551,795 + BT0451 0.72 -1.5 551,797 + BT0451 0.72 -2.4 551,797 + BT0451 0.72 -2.1 551,797 + BT0451 0.72 -0.7 551,912 + BT0451 0.79 -1.7 552,003 + BT0451 0.84 +0.9 552,104 + -1.1 552,197 + -1.8 552,204 + -0.1 552,205 - -2.1 552,205 - -1.7 552,264 + -1.7 552,332 + -1.4 552,332 + -0.5 552,409 + -0.1 552,465 + -2.1 552,465 + +0.9 552,465 + -1.1 552,529 + +2.2 552,604 - +0.9 552,623 + BT0452 0.11 -1.8 552,626 - BT0452 0.11 -0.5 552,785 + BT0452 0.16 -1.7 552,955 + BT0452 0.21 +0.9 552,970 + BT0452 0.21 +1.5 553,160 + BT0452 0.27 -1.7 553,165 - BT0452 0.27 -2.0 553,169 + -1.1 553,223 + BT0452 0.29 +1.5 553,224 - BT0452 0.29 -0.1 553,229 + BT0452 0.29 -0.1 553,230 - BT0452 0.29 -0.1 553,236 + BT0452 0.30 +0.9 553,298 + BT0452 0.31 -1.5 553,299 - BT0452 0.32 -1.1 553,418 + BT0452 0.35 +0.9 553,423 - BT0452 0.35 -2.1 553,582 + BT0452 0.40 -1.7 553,641 + BT0452 0.42 -0.1 553,650 - BT0452 0.42 +0.5 553,683 + BT0452 0.43 -0.1 553,819 + BT0452 0.48 -0.8 553,819 + BT0452 0.48 -1.1 553,989 + BT0452 0.53 -0.8 554,065 + BT0452 0.55 -1.4 554,094 + BT0452 0.56 -0.7 554,094 + BT0452 0.56 -0.9 554,096 + BT0452 0.56 -1.9 554,097 - BT0452 0.56 -0.6 554,203 - BT0452 0.60 -1.3 554,256 + BT0452 0.61 +0.2 554,314 - BT0452 0.63 +1.5 554,374 + BT0452 0.65 -1.2 554,423 + BT0452 0.66 -1.7 554,433 - BT0452 0.67 -0.3 554,451 + BT0452 0.67 +0.2 554,544 + BT0452 0.70 +1.5 554,565 - BT0452 0.71 -0.1 554,689 - BT0452 0.75 -2.4 554,700 + BT0452 0.75 -1.3 554,764 + BT0452 0.77 -0.1 554,775 + BT0452 0.77 -0.1 554,852 + BT0452 0.80 -2.3 554,887 + BT0452 0.81 -1.5 554,904 + BT0452 0.81 -1.7 554,948 + BT0452 0.83 -3.7 554,949 - BT0452 0.83 -1.1 555,247 + -0.1 555,247 + -1.1 555,248 - -1.1 555,267 + +0.3 555,319 - -0.1 555,319 - -1.7 555,327 + -1.7 555,595 + -2.1 555,793 + BT0453 0.20 -1.1 556,194 + BT0453 0.54 -0.1 556,343 - BT0453 0.67 -0.1 556,444 - BT0453 0.76 +0.2
Or see this region's nucleotide sequence