Experiment: Sucrose (C) and Ammonium chloride (N); with TAPS; with PEG
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS29495 and PFLU_RS29500 are separated by 10 nucleotides PFLU_RS29500 and PFLU_RS29505 are separated by 167 nucleotides PFLU_RS29505 and PFLU_RS29510 are separated by 60 nucleotides
PFLU_RS29495: PFLU_RS29495 - YicC family protein, at 6,547,661 to 6,548,524
_RS29495
PFLU_RS29500: PFLU_RS29500 - guanylate kinase, at 6,548,535 to 6,549,155
_RS29500
PFLU_RS29505: PFLU_RS29505 - DNA-directed RNA polymerase subunit omega, at 6,549,323 to 6,549,586
_RS29505
PFLU_RS29510: PFLU_RS29510 - bifunctional GTP diphosphokinase/guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase, at 6,549,647 to 6,551,752
_RS29510
Position (kb)
6549
6550 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6548.422 kb on + strand, within PFLU_RS29495 at 6548.422 kb on + strand, within PFLU_RS29495 at 6548.422 kb on + strand, within PFLU_RS29495 at 6548.422 kb on + strand, within PFLU_RS29495 at 6548.423 kb on - strand, within PFLU_RS29495 at 6548.423 kb on - strand, within PFLU_RS29495 at 6548.423 kb on - strand, within PFLU_RS29495 at 6548.423 kb on - strand, within PFLU_RS29495 at 6548.423 kb on - strand, within PFLU_RS29495 at 6548.423 kb on - strand, within PFLU_RS29495 at 6548.423 kb on - strand, within PFLU_RS29495 at 6548.508 kb on + strand at 6548.508 kb on + strand at 6548.508 kb on + strand at 6548.508 kb on + strand at 6548.509 kb on - strand at 6548.509 kb on - strand at 6548.509 kb on - strand at 6548.509 kb on - strand at 6548.515 kb on + strand at 6548.515 kb on + strand at 6548.522 kb on + strand at 6549.133 kb on + strand at 6549.187 kb on + strand at 6549.187 kb on + strand at 6549.187 kb on + strand at 6549.188 kb on - strand at 6549.188 kb on - strand at 6549.188 kb on - strand at 6549.188 kb on - strand at 6549.188 kb on - strand at 6549.188 kb on - strand at 6549.188 kb on - strand at 6549.268 kb on + strand at 6549.269 kb on - strand at 6549.319 kb on + strand at 6549.319 kb on + strand at 6549.319 kb on + strand at 6549.319 kb on + strand at 6549.319 kb on + strand at 6549.319 kb on + strand at 6549.319 kb on + strand at 6549.319 kb on + strand at 6549.320 kb on - strand at 6549.320 kb on - strand at 6549.351 kb on + strand, within PFLU_RS29505 at 6549.351 kb on + strand, within PFLU_RS29505 at 6549.351 kb on + strand, within PFLU_RS29505 at 6549.351 kb on + strand, within PFLU_RS29505 at 6549.351 kb on + strand, within PFLU_RS29505 at 6549.351 kb on + strand, within PFLU_RS29505 at 6549.351 kb on + strand, within PFLU_RS29505 at 6549.391 kb on + strand, within PFLU_RS29505 at 6549.460 kb on + strand, within PFLU_RS29505 at 6549.460 kb on + strand, within PFLU_RS29505 at 6549.460 kb on + strand, within PFLU_RS29505 at 6549.468 kb on + strand, within PFLU_RS29505 at 6549.468 kb on + strand, within PFLU_RS29505 at 6549.503 kb on + strand, within PFLU_RS29505 at 6549.503 kb on + strand, within PFLU_RS29505 at 6549.503 kb on + strand, within PFLU_RS29505 at 6549.503 kb on + strand, within PFLU_RS29505 at 6549.503 kb on + strand, within PFLU_RS29505 at 6549.504 kb on - strand, within PFLU_RS29505 at 6549.584 kb on + strand at 6549.584 kb on + strand at 6549.590 kb on + strand at 6549.590 kb on + strand at 6549.590 kb on + strand at 6549.590 kb on + strand at 6549.590 kb on + strand at 6549.590 kb on + strand at 6549.605 kb on + strand at 6549.605 kb on + strand at 6549.605 kb on + strand at 6549.657 kb on + strand at 6549.658 kb on - strand at 6549.658 kb on - strand at 6549.658 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sucrose (C) and Ammonium chloride (N); with TAPS; with PEG remove 6,548,422 + PFLU_RS29495 0.88 -1.3 6,548,422 + PFLU_RS29495 0.88 +0.2 6,548,422 + PFLU_RS29495 0.88 +0.5 6,548,422 + PFLU_RS29495 0.88 -0.3 6,548,423 - PFLU_RS29495 0.88 +0.5 6,548,423 - PFLU_RS29495 0.88 -0.9 6,548,423 - PFLU_RS29495 0.88 +0.5 6,548,423 - PFLU_RS29495 0.88 +0.9 6,548,423 - PFLU_RS29495 0.88 -0.2 6,548,423 - PFLU_RS29495 0.88 +0.1 6,548,423 - PFLU_RS29495 0.88 +1.4 6,548,508 + -1.3 6,548,508 + -1.3 6,548,508 + +0.1 6,548,508 + +0.1 6,548,509 - -0.3 6,548,509 - -0.6 6,548,509 - +1.6 6,548,509 - -0.4 6,548,515 + +1.5 6,548,515 + -0.1 6,548,522 + -0.4 6,549,133 + -0.1 6,549,187 + -0.1 6,549,187 + +0.8 6,549,187 + +0.4 6,549,188 - -0.7 6,549,188 - +1.0 6,549,188 - +0.8 6,549,188 - +0.2 6,549,188 - +0.1 6,549,188 - -0.5 6,549,188 - +0.7 6,549,268 + +0.1 6,549,269 - -0.8 6,549,319 + +0.2 6,549,319 + -0.5 6,549,319 + -0.8 6,549,319 + -1.3 6,549,319 + +1.9 6,549,319 + -2.4 6,549,319 + +0.1 6,549,319 + -0.7 6,549,320 - +0.3 6,549,320 - +1.1 6,549,351 + PFLU_RS29505 0.11 +1.1 6,549,351 + PFLU_RS29505 0.11 -0.5 6,549,351 + PFLU_RS29505 0.11 -1.3 6,549,351 + PFLU_RS29505 0.11 -1.2 6,549,351 + PFLU_RS29505 0.11 -2.2 6,549,351 + PFLU_RS29505 0.11 -0.4 6,549,351 + PFLU_RS29505 0.11 +0.4 6,549,391 + PFLU_RS29505 0.26 -0.9 6,549,460 + PFLU_RS29505 0.52 -0.2 6,549,460 + PFLU_RS29505 0.52 -3.0 6,549,460 + PFLU_RS29505 0.52 -1.7 6,549,468 + PFLU_RS29505 0.55 -2.4 6,549,468 + PFLU_RS29505 0.55 -0.9 6,549,503 + PFLU_RS29505 0.68 -1.5 6,549,503 + PFLU_RS29505 0.68 -0.3 6,549,503 + PFLU_RS29505 0.68 +0.6 6,549,503 + PFLU_RS29505 0.68 -1.7 6,549,503 + PFLU_RS29505 0.68 -1.2 6,549,504 - PFLU_RS29505 0.69 -0.5 6,549,584 + +2.5 6,549,584 + +1.2 6,549,590 + -0.7 6,549,590 + +1.4 6,549,590 + +0.7 6,549,590 + -0.9 6,549,590 + +0.4 6,549,590 + -0.2 6,549,605 + +0.7 6,549,605 + -1.4 6,549,605 + -0.0 6,549,657 + -0.6 6,549,658 - +1.0 6,549,658 - +0.3 6,549,658 - +0.1
Or see this region's nucleotide sequence