Experiment: Sucrose (C) and Ammonium chloride (N); with TAPS; with PEG
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS14295 and PFLU_RS14300 overlap by 4 nucleotides PFLU_RS14300 and PFLU_RS14305 overlap by 4 nucleotides PFLU_RS14305 and PFLU_RS14310 are separated by 170 nucleotides PFLU_RS14310 and PFLU_RS14315 are separated by 57 nucleotides
PFLU_RS14295: PFLU_RS14295 - PAS domain S-box protein, at 3,203,836 to 3,206,151
_RS14295
PFLU_RS14300: PFLU_RS14300 - response regulator transcription factor, at 3,206,148 to 3,206,801
_RS14300
PFLU_RS14305: PFLU_RS14305 - sensor histidine kinase, at 3,206,798 to 3,207,982
_RS14305
PFLU_RS14310: PFLU_RS14310 - SgcJ/EcaC family oxidoreductase, at 3,208,153 to 3,208,671
_RS14310
PFLU_RS14315: PFLU_RS14315 - TonB-dependent receptor, at 3,208,729 to 3,210,804
_RS14315
Position (kb)
3206
3207
3208 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3205.975 kb on + strand at 3206.005 kb on + strand at 3206.006 kb on - strand at 3206.054 kb on + strand at 3206.054 kb on + strand at 3206.054 kb on + strand at 3206.054 kb on + strand at 3206.054 kb on + strand at 3206.055 kb on - strand at 3206.055 kb on - strand at 3206.055 kb on - strand at 3206.262 kb on - strand, within PFLU_RS14300 at 3206.491 kb on + strand, within PFLU_RS14300 at 3206.492 kb on - strand, within PFLU_RS14300 at 3206.492 kb on - strand, within PFLU_RS14300 at 3206.987 kb on + strand, within PFLU_RS14305 at 3207.065 kb on - strand, within PFLU_RS14305 at 3207.399 kb on + strand, within PFLU_RS14305 at 3207.399 kb on + strand, within PFLU_RS14305 at 3208.029 kb on - strand at 3208.031 kb on + strand at 3208.032 kb on - strand at 3208.032 kb on - strand at 3208.032 kb on - strand at 3208.073 kb on + strand at 3208.073 kb on + strand at 3208.231 kb on + strand, within PFLU_RS14310 at 3208.232 kb on - strand, within PFLU_RS14310 at 3208.232 kb on - strand, within PFLU_RS14310 at 3208.351 kb on + strand, within PFLU_RS14310 at 3208.351 kb on + strand, within PFLU_RS14310 at 3208.351 kb on + strand, within PFLU_RS14310 at 3208.351 kb on + strand, within PFLU_RS14310 at 3208.351 kb on + strand, within PFLU_RS14310 at 3208.351 kb on + strand, within PFLU_RS14310 at 3208.351 kb on + strand, within PFLU_RS14310 at 3208.351 kb on + strand, within PFLU_RS14310 at 3208.351 kb on + strand, within PFLU_RS14310 at 3208.351 kb on + strand, within PFLU_RS14310 at 3208.351 kb on + strand, within PFLU_RS14310 at 3208.351 kb on + strand, within PFLU_RS14310 at 3208.352 kb on - strand, within PFLU_RS14310 at 3208.352 kb on - strand, within PFLU_RS14310 at 3208.352 kb on - strand, within PFLU_RS14310 at 3208.352 kb on - strand, within PFLU_RS14310 at 3208.352 kb on - strand, within PFLU_RS14310 at 3208.352 kb on - strand, within PFLU_RS14310 at 3208.352 kb on - strand, within PFLU_RS14310 at 3208.352 kb on - strand, within PFLU_RS14310 at 3208.423 kb on + strand, within PFLU_RS14310 at 3208.423 kb on + strand, within PFLU_RS14310 at 3208.468 kb on + strand, within PFLU_RS14310 at 3208.469 kb on - strand, within PFLU_RS14310 at 3208.469 kb on - strand, within PFLU_RS14310 at 3208.494 kb on + strand, within PFLU_RS14310 at 3208.520 kb on - strand, within PFLU_RS14310 at 3208.585 kb on + strand, within PFLU_RS14310 at 3208.585 kb on + strand, within PFLU_RS14310 at 3208.586 kb on - strand, within PFLU_RS14310 at 3208.586 kb on - strand, within PFLU_RS14310 at 3208.754 kb on + strand at 3208.754 kb on + strand at 3208.754 kb on + strand at 3208.754 kb on + strand at 3208.811 kb on + strand at 3208.811 kb on + strand at 3208.811 kb on + strand at 3208.820 kb on + strand at 3208.820 kb on + strand at 3208.820 kb on + strand at 3208.821 kb on - strand at 3208.826 kb on + strand at 3208.826 kb on + strand at 3208.827 kb on - strand at 3208.827 kb on - strand at 3208.827 kb on - strand at 3208.827 kb on - strand at 3208.827 kb on - strand at 3208.827 kb on - strand at 3208.827 kb on - strand at 3208.827 kb on - strand at 3208.886 kb on + strand at 3208.887 kb on - strand at 3208.887 kb on - strand at 3208.904 kb on - strand at 3208.914 kb on - strand at 3208.914 kb on - strand at 3208.914 kb on - strand at 3208.952 kb on + strand, within PFLU_RS14315 at 3208.953 kb on - strand, within PFLU_RS14315 at 3208.953 kb on - strand, within PFLU_RS14315 at 3208.953 kb on - strand, within PFLU_RS14315 at 3208.953 kb on - strand, within PFLU_RS14315 at 3208.955 kb on + strand, within PFLU_RS14315 at 3208.955 kb on + strand, within PFLU_RS14315 at 3208.955 kb on + strand, within PFLU_RS14315 at 3208.955 kb on + strand, within PFLU_RS14315 at 3208.955 kb on + strand, within PFLU_RS14315 at 3208.955 kb on + strand, within PFLU_RS14315 at 3208.955 kb on + strand, within PFLU_RS14315 at 3208.956 kb on - strand, within PFLU_RS14315 at 3208.956 kb on - strand, within PFLU_RS14315 at 3208.956 kb on - strand, within PFLU_RS14315 at 3208.956 kb on - strand, within PFLU_RS14315 at 3208.956 kb on - strand, within PFLU_RS14315 at 3208.956 kb on - strand, within PFLU_RS14315 at 3208.956 kb on - strand, within PFLU_RS14315
Per-strain Table
Position Strand Gene LocusTag Fraction Sucrose (C) and Ammonium chloride (N); with TAPS; with PEG remove 3,205,975 + +1.6 3,206,005 + -0.1 3,206,006 - +0.6 3,206,054 + +0.3 3,206,054 + -0.1 3,206,054 + -0.5 3,206,054 + +1.4 3,206,054 + +1.7 3,206,055 - +0.3 3,206,055 - +0.7 3,206,055 - -0.6 3,206,262 - PFLU_RS14300 0.17 +0.4 3,206,491 + PFLU_RS14300 0.52 +0.6 3,206,492 - PFLU_RS14300 0.53 -0.7 3,206,492 - PFLU_RS14300 0.53 -0.4 3,206,987 + PFLU_RS14305 0.16 -0.5 3,207,065 - PFLU_RS14305 0.23 -0.9 3,207,399 + PFLU_RS14305 0.51 -1.2 3,207,399 + PFLU_RS14305 0.51 -1.2 3,208,029 - -0.4 3,208,031 + +0.9 3,208,032 - -0.3 3,208,032 - -1.6 3,208,032 - -2.2 3,208,073 + -0.2 3,208,073 + -1.1 3,208,231 + PFLU_RS14310 0.15 +0.7 3,208,232 - PFLU_RS14310 0.15 -0.3 3,208,232 - PFLU_RS14310 0.15 -0.4 3,208,351 + PFLU_RS14310 0.38 -0.3 3,208,351 + PFLU_RS14310 0.38 -0.1 3,208,351 + PFLU_RS14310 0.38 +0.5 3,208,351 + PFLU_RS14310 0.38 +0.6 3,208,351 + PFLU_RS14310 0.38 -1.3 3,208,351 + PFLU_RS14310 0.38 +0.6 3,208,351 + PFLU_RS14310 0.38 -0.2 3,208,351 + PFLU_RS14310 0.38 -2.2 3,208,351 + PFLU_RS14310 0.38 -0.8 3,208,351 + PFLU_RS14310 0.38 +0.4 3,208,351 + PFLU_RS14310 0.38 +0.1 3,208,351 + PFLU_RS14310 0.38 +1.7 3,208,352 - PFLU_RS14310 0.38 +0.1 3,208,352 - PFLU_RS14310 0.38 +0.9 3,208,352 - PFLU_RS14310 0.38 +0.5 3,208,352 - PFLU_RS14310 0.38 -0.7 3,208,352 - PFLU_RS14310 0.38 +0.7 3,208,352 - PFLU_RS14310 0.38 +1.1 3,208,352 - PFLU_RS14310 0.38 -0.6 3,208,352 - PFLU_RS14310 0.38 -0.7 3,208,423 + PFLU_RS14310 0.52 -2.9 3,208,423 + PFLU_RS14310 0.52 -0.5 3,208,468 + PFLU_RS14310 0.61 -0.1 3,208,469 - PFLU_RS14310 0.61 -0.9 3,208,469 - PFLU_RS14310 0.61 +0.1 3,208,494 + PFLU_RS14310 0.66 -1.6 3,208,520 - PFLU_RS14310 0.71 -0.5 3,208,585 + PFLU_RS14310 0.83 +1.5 3,208,585 + PFLU_RS14310 0.83 -0.9 3,208,586 - PFLU_RS14310 0.83 -1.9 3,208,586 - PFLU_RS14310 0.83 +1.1 3,208,754 + -1.4 3,208,754 + +0.1 3,208,754 + -0.2 3,208,754 + -0.3 3,208,811 + +1.5 3,208,811 + +0.1 3,208,811 + +1.0 3,208,820 + +0.8 3,208,820 + +0.3 3,208,820 + +0.1 3,208,821 - +0.8 3,208,826 + +0.6 3,208,826 + +1.1 3,208,827 - +0.3 3,208,827 - -0.5 3,208,827 - +0.6 3,208,827 - -1.3 3,208,827 - +0.5 3,208,827 - -0.2 3,208,827 - -1.6 3,208,827 - -1.6 3,208,886 + +0.8 3,208,887 - -0.1 3,208,887 - -0.4 3,208,904 - +0.2 3,208,914 - +1.0 3,208,914 - +1.6 3,208,914 - +1.8 3,208,952 + PFLU_RS14315 0.11 +0.7 3,208,953 - PFLU_RS14315 0.11 -0.7 3,208,953 - PFLU_RS14315 0.11 -0.5 3,208,953 - PFLU_RS14315 0.11 +1.3 3,208,953 - PFLU_RS14315 0.11 -1.4 3,208,955 + PFLU_RS14315 0.11 +1.0 3,208,955 + PFLU_RS14315 0.11 +1.2 3,208,955 + PFLU_RS14315 0.11 -0.4 3,208,955 + PFLU_RS14315 0.11 -1.6 3,208,955 + PFLU_RS14315 0.11 -0.5 3,208,955 + PFLU_RS14315 0.11 +0.1 3,208,955 + PFLU_RS14315 0.11 +0.7 3,208,956 - PFLU_RS14315 0.11 -0.4 3,208,956 - PFLU_RS14315 0.11 +0.6 3,208,956 - PFLU_RS14315 0.11 -1.1 3,208,956 - PFLU_RS14315 0.11 -0.9 3,208,956 - PFLU_RS14315 0.11 +0.3 3,208,956 - PFLU_RS14315 0.11 -1.9 3,208,956 - PFLU_RS14315 0.11 -1.7
Or see this region's nucleotide sequence