Experiment: Varel_Bryant_medium_Glucose with Doxycycline hyclate 0.0001953125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0131 and BT0132 are separated by 239 nucleotides BT0132 and BT0133 overlap by 17 nucleotides BT0133 and BT0134 are separated by 10 nucleotides
BT0131: BT0131 - putative transposase (NCBI ptt file), at 126,746 to 127,678
BT0131
BT0132: BT0132 - alpha-glucosidase, putative (NCBI ptt file), at 127,918 to 129,852
BT0132
BT0133: BT0133 - putative sugar phosphate isomerase (NCBI ptt file), at 129,836 to 130,669
BT0133
BT0134: BT0134 - conserved hypothetical protein (NCBI ptt file), at 130,680 to 131,063
BT0134
Position (kb)
127
128
129
130 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 127.543 kb on + strand, within BT0131 at 127.860 kb on - strand at 127.862 kb on - strand at 127.864 kb on + strand at 127.880 kb on - strand at 127.882 kb on - strand at 127.884 kb on - strand at 127.930 kb on - strand at 127.953 kb on - strand at 128.080 kb on - strand at 128.087 kb on + strand at 128.087 kb on + strand at 128.087 kb on + strand at 128.089 kb on + strand at 128.091 kb on + strand at 128.091 kb on + strand at 128.255 kb on + strand, within BT0132 at 128.289 kb on + strand, within BT0132 at 128.377 kb on - strand, within BT0132 at 128.455 kb on - strand, within BT0132 at 128.457 kb on + strand, within BT0132 at 128.504 kb on - strand, within BT0132 at 128.530 kb on + strand, within BT0132 at 128.695 kb on - strand at 128.719 kb on - strand, within BT0132 at 128.729 kb on - strand, within BT0132 at 128.940 kb on + strand, within BT0132 at 129.071 kb on - strand, within BT0132 at 129.167 kb on + strand, within BT0132 at 129.186 kb on + strand, within BT0132 at 129.194 kb on + strand, within BT0132 at 129.345 kb on + strand, within BT0132 at 129.360 kb on + strand, within BT0132 at 129.389 kb on - strand, within BT0132 at 129.390 kb on + strand, within BT0132 at 129.455 kb on + strand, within BT0132 at 129.456 kb on - strand, within BT0132 at 129.461 kb on - strand, within BT0132 at 129.505 kb on + strand, within BT0132 at 129.505 kb on + strand, within BT0132 at 129.507 kb on + strand, within BT0132 at 129.572 kb on + strand, within BT0132 at 129.585 kb on - strand, within BT0132 at 129.592 kb on + strand, within BT0132 at 129.593 kb on - strand, within BT0132 at 129.648 kb on + strand, within BT0132 at 129.667 kb on - strand at 129.703 kb on + strand at 129.703 kb on + strand at 129.705 kb on + strand at 129.706 kb on - strand at 129.718 kb on + strand at 129.742 kb on - strand at 129.742 kb on - strand at 129.790 kb on - strand at 129.887 kb on - strand at 130.009 kb on + strand, within BT0133 at 130.240 kb on - strand, within BT0133 at 130.276 kb on + strand at 130.277 kb on - strand, within BT0133 at 130.277 kb on - strand, within BT0133 at 130.291 kb on + strand, within BT0133 at 130.327 kb on + strand, within BT0133 at 130.394 kb on + strand, within BT0133 at 130.394 kb on + strand, within BT0133 at 130.395 kb on - strand, within BT0133 at 130.395 kb on - strand, within BT0133 at 130.408 kb on + strand, within BT0133 at 130.482 kb on - strand, within BT0133 at 130.482 kb on - strand, within BT0133 at 130.583 kb on - strand, within BT0133 at 130.681 kb on + strand at 130.689 kb on + strand at 130.716 kb on - strand at 130.822 kb on - strand, within BT0134 at 130.822 kb on - strand, within BT0134 at 130.838 kb on + strand, within BT0134
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Doxycycline hyclate 0.0001953125 mM remove 127,543 + BT0131 0.85 -1.7 127,860 - -1.4 127,862 - -1.6 127,864 + -0.1 127,880 - -0.1 127,882 - +0.5 127,884 - -0.0 127,930 - -0.6 127,953 - +0.9 128,080 - -0.5 128,087 + -1.1 128,087 + +0.9 128,087 + -0.7 128,089 + +0.2 128,091 + -0.1 128,091 + -0.1 128,255 + BT0132 0.17 -0.1 128,289 + BT0132 0.19 -0.1 128,377 - BT0132 0.24 +0.3 128,455 - BT0132 0.28 +1.5 128,457 + BT0132 0.28 +1.9 128,504 - BT0132 0.30 +2.2 128,530 + BT0132 0.32 -0.3 128,695 - -1.1 128,719 - BT0132 0.41 -1.3 128,729 - BT0132 0.42 +2.0 128,940 + BT0132 0.53 -1.1 129,071 - BT0132 0.60 +1.7 129,167 + BT0132 0.65 -0.1 129,186 + BT0132 0.66 +0.2 129,194 + BT0132 0.66 -0.1 129,345 + BT0132 0.74 +0.9 129,360 + BT0132 0.75 -1.7 129,389 - BT0132 0.76 -2.1 129,390 + BT0132 0.76 -0.1 129,455 + BT0132 0.79 -0.1 129,456 - BT0132 0.79 +1.5 129,461 - BT0132 0.80 -0.1 129,505 + BT0132 0.82 -1.8 129,505 + BT0132 0.82 -0.7 129,507 + BT0132 0.82 +1.2 129,572 + BT0132 0.85 +0.1 129,585 - BT0132 0.86 +1.2 129,592 + BT0132 0.87 +0.6 129,593 - BT0132 0.87 -0.9 129,648 + BT0132 0.89 -0.1 129,667 - -0.1 129,703 + -0.7 129,703 + -1.4 129,705 + -0.5 129,706 - +1.5 129,718 + -1.1 129,742 - -0.7 129,742 - -1.7 129,790 - -0.3 129,887 - -0.1 130,009 + BT0133 0.21 -1.5 130,240 - BT0133 0.48 -1.4 130,276 + -0.1 130,277 - BT0133 0.53 -0.5 130,277 - BT0133 0.53 -0.3 130,291 + BT0133 0.55 -1.7 130,327 + BT0133 0.59 -1.1 130,394 + BT0133 0.67 +0.9 130,394 + BT0133 0.67 +0.9 130,395 - BT0133 0.67 -1.1 130,395 - BT0133 0.67 +1.2 130,408 + BT0133 0.69 -1.7 130,482 - BT0133 0.77 +0.2 130,482 - BT0133 0.77 +0.9 130,583 - BT0133 0.90 +1.2 130,681 + +1.5 130,689 + +1.5 130,716 - -2.7 130,822 - BT0134 0.37 -0.9 130,822 - BT0134 0.37 -0.6 130,838 + BT0134 0.41 -0.1
Or see this region's nucleotide sequence