Strain Fitness in Ralstonia sp. UNC404CL21Col around ABZR87_RS00065

Experiment: Disodium Glutarate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntABZR87_RS00055 and ABZR87_RS00060 are separated by 10 nucleotidesABZR87_RS00060 and ABZR87_RS00065 are separated by 12 nucleotidesABZR87_RS00065 and ABZR87_RS00070 overlap by 16 nucleotidesABZR87_RS00070 and ABZR87_RS00075 overlap by 4 nucleotides ABZR87_RS00055: ABZR87_RS00055 - threonine-phosphate decarboxylase CobD, at 13,001 to 14,008 _RS00055 ABZR87_RS00060: ABZR87_RS00060 - cobalamin-binding protein, at 14,019 to 14,975 _RS00060 ABZR87_RS00065: ABZR87_RS00065 - alpha-ribazole phosphatase, at 14,988 to 15,575 _RS00065 ABZR87_RS00070: ABZR87_RS00070 - adenosylcobinamide-GDP ribazoletransferase, at 15,560 to 16,330 _RS00070 ABZR87_RS00075: ABZR87_RS00075 - nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase, at 16,327 to 17,391 _RS00075 Position (kb) 14 15 16Strain fitness (log2 ratio) -2 -1 0 1 2 3at 14.055 kb on + strandat 14.055 kb on + strandat 14.055 kb on + strandat 14.055 kb on + strandat 14.055 kb on + strandat 14.055 kb on + strandat 14.055 kb on + strandat 14.056 kb on - strandat 14.056 kb on - strandat 14.056 kb on - strandat 14.056 kb on - strandat 14.056 kb on - strandat 14.056 kb on - strandat 14.056 kb on - strandat 14.056 kb on - strandat 14.056 kb on - strandat 14.056 kb on - strandat 14.251 kb on - strand, within ABZR87_RS00060at 14.444 kb on + strand, within ABZR87_RS00060at 14.444 kb on + strand, within ABZR87_RS00060at 14.580 kb on + strand, within ABZR87_RS00060at 14.580 kb on + strand, within ABZR87_RS00060at 14.580 kb on + strand, within ABZR87_RS00060at 14.580 kb on + strand, within ABZR87_RS00060at 14.580 kb on + strand, within ABZR87_RS00060at 14.580 kb on + strand, within ABZR87_RS00060at 14.580 kb on + strand, within ABZR87_RS00060at 14.581 kb on - strand, within ABZR87_RS00060at 14.581 kb on - strand, within ABZR87_RS00060at 14.581 kb on - strand, within ABZR87_RS00060at 15.831 kb on + strand, within ABZR87_RS00070at 15.831 kb on + strand, within ABZR87_RS00070at 15.831 kb on + strand, within ABZR87_RS00070at 15.831 kb on + strand, within ABZR87_RS00070at 15.832 kb on - strand, within ABZR87_RS00070at 15.832 kb on - strand, within ABZR87_RS00070at 15.832 kb on - strand, within ABZR87_RS00070at 15.832 kb on - strand, within ABZR87_RS00070at 15.832 kb on - strand, within ABZR87_RS00070at 16.164 kb on + strand, within ABZR87_RS00070at 16.165 kb on - strand, within ABZR87_RS00070at 16.282 kb on - strandat 16.282 kb on - strandat 16.282 kb on - strandat 16.516 kb on + strand, within ABZR87_RS00075at 16.516 kb on + strand, within ABZR87_RS00075at 16.516 kb on + strand, within ABZR87_RS00075at 16.516 kb on + strand, within ABZR87_RS00075at 16.517 kb on - strand, within ABZR87_RS00075at 16.517 kb on - strand, within ABZR87_RS00075at 16.517 kb on - strand, within ABZR87_RS00075at 16.535 kb on + strand, within ABZR87_RS00075

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Per-strain Table

Position Strand Gene LocusTag Fraction Disodium Glutarate (C)
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14,055 + -1.0
14,055 + -0.1
14,055 + -0.6
14,055 + -1.2
14,055 + -0.0
14,055 + -1.2
14,055 + +0.4
14,056 - -0.3
14,056 - +0.2
14,056 - -0.8
14,056 - +2.9
14,056 - +2.7
14,056 - +0.1
14,056 - +0.3
14,056 - -0.4
14,056 - -0.3
14,056 - -0.0
14,251 - ABZR87_RS00060 0.24 +1.5
14,444 + ABZR87_RS00060 0.44 +0.3
14,444 + ABZR87_RS00060 0.44 +0.7
14,580 + ABZR87_RS00060 0.59 +0.5
14,580 + ABZR87_RS00060 0.59 +0.4
14,580 + ABZR87_RS00060 0.59 +0.3
14,580 + ABZR87_RS00060 0.59 +0.3
14,580 + ABZR87_RS00060 0.59 +0.2
14,580 + ABZR87_RS00060 0.59 +1.1
14,580 + ABZR87_RS00060 0.59 +0.2
14,581 - ABZR87_RS00060 0.59 +0.4
14,581 - ABZR87_RS00060 0.59 +1.0
14,581 - ABZR87_RS00060 0.59 +0.0
15,831 + ABZR87_RS00070 0.35 -0.5
15,831 + ABZR87_RS00070 0.35 +0.3
15,831 + ABZR87_RS00070 0.35 +0.8
15,831 + ABZR87_RS00070 0.35 +0.5
15,832 - ABZR87_RS00070 0.35 -0.1
15,832 - ABZR87_RS00070 0.35 +1.0
15,832 - ABZR87_RS00070 0.35 +0.5
15,832 - ABZR87_RS00070 0.35 -0.6
15,832 - ABZR87_RS00070 0.35 +1.5
16,164 + ABZR87_RS00070 0.78 +0.1
16,165 - ABZR87_RS00070 0.78 -1.8
16,282 - +1.0
16,282 - +1.5
16,282 - +0.3
16,516 + ABZR87_RS00075 0.18 -0.7
16,516 + ABZR87_RS00075 0.18 -0.4
16,516 + ABZR87_RS00075 0.18 -2.1
16,516 + ABZR87_RS00075 0.18 -0.5
16,517 - ABZR87_RS00075 0.18 +0.2
16,517 - ABZR87_RS00075 0.18 +0.3
16,517 - ABZR87_RS00075 0.18 +1.2
16,535 + ABZR87_RS00075 0.20 -1.4

Or see this region's nucleotide sequence