Experiment: Varel_Bryant_medium_Glucose with Spiramycin 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3728 and BT3729 are separated by 71 nucleotides BT3729 and BT3730 overlap by 4 nucleotides BT3730 and BT3731 are separated by 77 nucleotides BT3731 and BT3732 are separated by 97 nucleotides
BT3728: BT3728 - riboflavin biosynthesis protein ribD (NCBI ptt file), at 4,838,863 to 4,839,882
BT3728
BT3729: BT3729 - putative protoporphyrinogen oxidase (NCBI ptt file), at 4,839,954 to 4,840,790
BT3729
BT3730: BT3730 - putative regulatory protein (NCBI ptt file), at 4,840,787 to 4,841,269
BT3730
BT3731: BT3731 - orotate phosphoribosyltransferase (NCBI ptt file), at 4,841,347 to 4,841,985
BT3731
BT3732: BT3732 - hypothetical protein (NCBI ptt file), at 4,842,083 to 4,842,493
BT3732
Position (kb)
4840
4841
4842 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4839.919 kb on - strand at 4839.922 kb on - strand at 4840.075 kb on - strand, within BT3729 at 4840.295 kb on - strand, within BT3729 at 4840.295 kb on - strand, within BT3729 at 4840.298 kb on + strand, within BT3729 at 4840.300 kb on + strand, within BT3729 at 4840.300 kb on + strand, within BT3729 at 4840.301 kb on - strand, within BT3729 at 4840.373 kb on + strand, within BT3729 at 4840.395 kb on - strand, within BT3729 at 4840.485 kb on + strand, within BT3729 at 4840.493 kb on - strand, within BT3729 at 4840.687 kb on - strand, within BT3729 at 4840.702 kb on + strand, within BT3729 at 4840.896 kb on + strand, within BT3730 at 4840.987 kb on + strand, within BT3730 at 4841.023 kb on - strand, within BT3730 at 4841.991 kb on + strand at 4842.036 kb on + strand at 4842.048 kb on + strand at 4842.068 kb on + strand at 4842.082 kb on + strand at 4842.120 kb on - strand at 4842.120 kb on - strand at 4842.138 kb on - strand, within BT3732
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Spiramycin 0.2 mM remove 4,839,919 - -1.0 4,839,922 - -1.2 4,840,075 - BT3729 0.14 -1.0 4,840,295 - BT3729 0.41 -2.6 4,840,295 - BT3729 0.41 -1.6 4,840,298 + BT3729 0.41 -1.0 4,840,300 + BT3729 0.41 -2.3 4,840,300 + BT3729 0.41 -2.0 4,840,301 - BT3729 0.41 -0.0 4,840,373 + BT3729 0.50 -0.0 4,840,395 - BT3729 0.53 -0.0 4,840,485 + BT3729 0.63 +1.6 4,840,493 - BT3729 0.64 +1.0 4,840,687 - BT3729 0.88 +1.6 4,840,702 + BT3729 0.89 -1.6 4,840,896 + BT3730 0.23 +1.0 4,840,987 + BT3730 0.41 +3.0 4,841,023 - BT3730 0.49 +0.6 4,841,991 + -0.4 4,842,036 + +1.0 4,842,048 + -2.0 4,842,068 + -1.2 4,842,082 + -0.0 4,842,120 - -2.3 4,842,120 - -1.6 4,842,138 - BT3732 0.13 -1.6
Or see this region's nucleotide sequence