Experiment: Varel_Bryant_medium_Glucose with Trimethoprim 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2512 and BT2513 are separated by 171 nucleotides BT2513 and BT2514 are separated by 43 nucleotides BT2514 and BT2515 are separated by 139 nucleotides BT2515 and BT2516 are separated by 68 nucleotides
BT2512: BT2512 - cation-transporting ATPase, P-type, putative zinc-transporting ATPase (NCBI ptt file), at 3,135,864 to 3,137,822
BT2512
BT2513: BT2513 - conserved hypothetical protein (NCBI ptt file), at 3,137,994 to 3,139,433
BT2513
BT2514: BT2514 - hypothetical protein (NCBI ptt file), at 3,139,477 to 3,139,674
BT2514
BT2515: BT2515 - hypothetical protein (NCBI ptt file), at 3,139,814 to 3,139,963
BT2515
BT2516: BT2516 - conserved hypothetical protein (NCBI ptt file), at 3,140,032 to 3,140,724
BT2516
Position (kb)
3137
3138
3139
3140 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3137.035 kb on + strand, within BT2512 at 3137.035 kb on + strand, within BT2512 at 3137.035 kb on + strand, within BT2512 at 3137.036 kb on - strand, within BT2512 at 3137.161 kb on - strand, within BT2512 at 3137.191 kb on - strand, within BT2512 at 3137.246 kb on + strand, within BT2512 at 3137.260 kb on - strand, within BT2512 at 3137.307 kb on + strand, within BT2512 at 3137.414 kb on + strand, within BT2512 at 3137.417 kb on + strand, within BT2512 at 3137.429 kb on + strand, within BT2512 at 3137.510 kb on - strand, within BT2512 at 3137.526 kb on - strand, within BT2512 at 3137.530 kb on - strand, within BT2512 at 3137.557 kb on + strand, within BT2512 at 3137.573 kb on + strand, within BT2512 at 3137.574 kb on - strand, within BT2512 at 3137.574 kb on - strand, within BT2512 at 3137.630 kb on - strand at 3137.667 kb on + strand at 3137.668 kb on - strand at 3137.698 kb on - strand at 3137.789 kb on + strand at 3137.805 kb on - strand at 3137.805 kb on - strand at 3137.822 kb on + strand at 3137.906 kb on + strand at 3137.911 kb on - strand at 3137.930 kb on + strand at 3137.930 kb on + strand at 3137.932 kb on + strand at 3137.988 kb on - strand at 3137.988 kb on - strand at 3137.990 kb on - strand at 3137.991 kb on + strand at 3138.002 kb on - strand at 3138.005 kb on + strand at 3138.008 kb on + strand at 3138.009 kb on - strand at 3138.010 kb on + strand at 3138.020 kb on - strand at 3138.022 kb on - strand at 3138.023 kb on + strand at 3138.102 kb on + strand at 3138.105 kb on - strand at 3138.110 kb on + strand at 3138.138 kb on + strand, within BT2513 at 3138.161 kb on - strand, within BT2513 at 3138.227 kb on - strand, within BT2513 at 3138.227 kb on - strand, within BT2513 at 3138.242 kb on - strand, within BT2513 at 3138.305 kb on + strand, within BT2513 at 3138.306 kb on - strand, within BT2513 at 3138.366 kb on - strand, within BT2513 at 3138.489 kb on + strand, within BT2513 at 3138.514 kb on + strand, within BT2513 at 3138.514 kb on + strand, within BT2513 at 3138.555 kb on + strand, within BT2513 at 3138.604 kb on + strand, within BT2513 at 3138.605 kb on - strand, within BT2513 at 3138.682 kb on - strand, within BT2513 at 3138.703 kb on + strand, within BT2513 at 3138.704 kb on - strand, within BT2513 at 3138.704 kb on - strand, within BT2513 at 3138.735 kb on - strand, within BT2513 at 3138.752 kb on + strand, within BT2513 at 3138.753 kb on - strand, within BT2513 at 3138.753 kb on - strand, within BT2513 at 3138.754 kb on + strand, within BT2513 at 3138.754 kb on + strand, within BT2513 at 3138.754 kb on + strand, within BT2513 at 3138.755 kb on - strand, within BT2513 at 3138.755 kb on - strand, within BT2513 at 3138.757 kb on + strand, within BT2513 at 3138.790 kb on + strand, within BT2513 at 3138.881 kb on + strand, within BT2513 at 3138.881 kb on + strand, within BT2513 at 3138.886 kb on - strand, within BT2513 at 3138.886 kb on - strand, within BT2513 at 3138.886 kb on - strand, within BT2513 at 3138.893 kb on + strand, within BT2513 at 3138.922 kb on + strand, within BT2513 at 3139.010 kb on + strand, within BT2513 at 3139.052 kb on + strand, within BT2513 at 3139.100 kb on + strand, within BT2513 at 3139.100 kb on + strand, within BT2513 at 3139.113 kb on + strand, within BT2513 at 3139.135 kb on + strand, within BT2513 at 3139.135 kb on + strand, within BT2513 at 3139.136 kb on - strand, within BT2513 at 3139.165 kb on - strand, within BT2513 at 3139.170 kb on - strand, within BT2513 at 3139.190 kb on - strand, within BT2513 at 3139.190 kb on - strand, within BT2513 at 3139.216 kb on - strand, within BT2513 at 3139.251 kb on - strand, within BT2513 at 3139.264 kb on - strand, within BT2513 at 3139.359 kb on + strand at 3139.359 kb on + strand at 3139.415 kb on + strand at 3139.416 kb on - strand at 3139.431 kb on + strand at 3139.446 kb on + strand at 3139.544 kb on + strand, within BT2514 at 3139.581 kb on - strand, within BT2514 at 3139.613 kb on + strand, within BT2514 at 3139.613 kb on + strand, within BT2514 at 3139.614 kb on - strand, within BT2514 at 3139.614 kb on - strand, within BT2514 at 3139.614 kb on - strand, within BT2514 at 3139.614 kb on - strand, within BT2514 at 3139.660 kb on + strand at 3139.661 kb on - strand at 3139.670 kb on + strand at 3139.704 kb on - strand at 3139.722 kb on - strand at 3139.724 kb on - strand at 3139.728 kb on - strand at 3139.738 kb on - strand at 3139.746 kb on + strand at 3139.747 kb on - strand at 3139.771 kb on + strand at 3139.772 kb on - strand at 3139.824 kb on + strand at 3139.824 kb on + strand at 3139.836 kb on - strand, within BT2515 at 3139.838 kb on - strand, within BT2515 at 3139.839 kb on + strand, within BT2515 at 3139.867 kb on + strand, within BT2515 at 3139.890 kb on - strand, within BT2515 at 3139.900 kb on + strand, within BT2515 at 3139.900 kb on + strand, within BT2515 at 3140.032 kb on - strand at 3140.092 kb on - strand at 3140.103 kb on + strand, within BT2516 at 3140.103 kb on + strand, within BT2516 at 3140.125 kb on + strand, within BT2516 at 3140.126 kb on - strand, within BT2516 at 3140.126 kb on - strand, within BT2516 at 3140.214 kb on - strand, within BT2516 at 3140.217 kb on + strand, within BT2516 at 3140.230 kb on - strand, within BT2516 at 3140.265 kb on - strand, within BT2516 at 3140.312 kb on + strand, within BT2516 at 3140.325 kb on + strand, within BT2516 at 3140.412 kb on + strand, within BT2516 at 3140.415 kb on - strand, within BT2516 at 3140.418 kb on - strand, within BT2516 at 3140.430 kb on - strand, within BT2516
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Trimethoprim 0.025 mM remove 3,137,035 + BT2512 0.60 +0.9 3,137,035 + BT2512 0.60 -3.2 3,137,035 + BT2512 0.60 -2.8 3,137,036 - BT2512 0.60 -4.4 3,137,161 - BT2512 0.66 -3.1 3,137,191 - BT2512 0.68 +0.4 3,137,246 + BT2512 0.71 -1.1 3,137,260 - BT2512 0.71 +1.9 3,137,307 + BT2512 0.74 +0.5 3,137,414 + BT2512 0.79 -0.5 3,137,417 + BT2512 0.79 -1.3 3,137,429 + BT2512 0.80 -2.2 3,137,510 - BT2512 0.84 -2.2 3,137,526 - BT2512 0.85 -1.6 3,137,530 - BT2512 0.85 -2.4 3,137,557 + BT2512 0.86 -0.4 3,137,573 + BT2512 0.87 +1.9 3,137,574 - BT2512 0.87 +2.5 3,137,574 - BT2512 0.87 -1.4 3,137,630 - +0.5 3,137,667 + +0.9 3,137,668 - +3.1 3,137,698 - -1.1 3,137,789 + -1.6 3,137,805 - -2.8 3,137,805 - -1.1 3,137,822 + +0.2 3,137,906 + +2.5 3,137,911 - -2.9 3,137,930 + -0.1 3,137,930 + -2.9 3,137,932 + -0.9 3,137,988 - +0.2 3,137,988 - -0.5 3,137,990 - -1.1 3,137,991 + -1.9 3,138,002 - -1.2 3,138,005 + -2.3 3,138,008 + -1.1 3,138,009 - -1.7 3,138,010 + -1.1 3,138,020 - +0.4 3,138,022 - -2.0 3,138,023 + -2.4 3,138,102 + -4.3 3,138,105 - +0.2 3,138,110 + -1.1 3,138,138 + BT2513 0.10 -0.8 3,138,161 - BT2513 0.12 +0.5 3,138,227 - BT2513 0.16 -0.9 3,138,227 - BT2513 0.16 -2.5 3,138,242 - BT2513 0.17 -0.1 3,138,305 + BT2513 0.22 +2.5 3,138,306 - BT2513 0.22 -1.6 3,138,366 - BT2513 0.26 +1.5 3,138,489 + BT2513 0.34 -2.1 3,138,514 + BT2513 0.36 +1.7 3,138,514 + BT2513 0.36 -2.2 3,138,555 + BT2513 0.39 +0.3 3,138,604 + BT2513 0.42 -0.1 3,138,605 - BT2513 0.42 +3.7 3,138,682 - BT2513 0.48 -1.1 3,138,703 + BT2513 0.49 -1.6 3,138,704 - BT2513 0.49 -1.0 3,138,704 - BT2513 0.49 +3.1 3,138,735 - BT2513 0.51 -1.6 3,138,752 + BT2513 0.53 -1.6 3,138,753 - BT2513 0.53 -2.1 3,138,753 - BT2513 0.53 -1.6 3,138,754 + BT2513 0.53 -1.6 3,138,754 + BT2513 0.53 -0.1 3,138,754 + BT2513 0.53 -1.1 3,138,755 - BT2513 0.53 -0.5 3,138,755 - BT2513 0.53 -2.9 3,138,757 + BT2513 0.53 -1.3 3,138,790 + BT2513 0.55 +1.5 3,138,881 + BT2513 0.62 -2.6 3,138,881 + BT2513 0.62 -1.1 3,138,886 - BT2513 0.62 -3.6 3,138,886 - BT2513 0.62 -2.6 3,138,886 - BT2513 0.62 -1.6 3,138,893 + BT2513 0.62 -0.1 3,138,922 + BT2513 0.64 -2.6 3,139,010 + BT2513 0.71 -2.6 3,139,052 + BT2513 0.73 -3.2 3,139,100 + BT2513 0.77 -1.3 3,139,100 + BT2513 0.77 -2.0 3,139,113 + BT2513 0.78 -2.6 3,139,135 + BT2513 0.79 -1.8 3,139,135 + BT2513 0.79 +3.5 3,139,136 - BT2513 0.79 -1.5 3,139,165 - BT2513 0.81 +1.9 3,139,170 - BT2513 0.82 -1.1 3,139,190 - BT2513 0.83 -1.1 3,139,190 - BT2513 0.83 -1.2 3,139,216 - BT2513 0.85 +1.8 3,139,251 - BT2513 0.87 -0.1 3,139,264 - BT2513 0.88 -1.3 3,139,359 + -1.1 3,139,359 + -2.7 3,139,415 + -0.1 3,139,416 - -0.6 3,139,431 + -2.4 3,139,446 + -3.4 3,139,544 + BT2514 0.34 -1.1 3,139,581 - BT2514 0.53 -3.2 3,139,613 + BT2514 0.69 +2.2 3,139,613 + BT2514 0.69 +1.3 3,139,614 - BT2514 0.69 -3.5 3,139,614 - BT2514 0.69 -0.8 3,139,614 - BT2514 0.69 -0.1 3,139,614 - BT2514 0.69 -1.6 3,139,660 + -3.6 3,139,661 - -0.7 3,139,670 + -1.8 3,139,704 - +1.4 3,139,722 - +1.5 3,139,724 - +0.9 3,139,728 - -1.8 3,139,738 - -1.1 3,139,746 + -1.9 3,139,747 - -1.6 3,139,771 + -3.2 3,139,772 - -3.5 3,139,824 + -1.6 3,139,824 + -1.9 3,139,836 - BT2515 0.15 -1.1 3,139,838 - BT2515 0.16 -1.9 3,139,839 + BT2515 0.17 -1.3 3,139,867 + BT2515 0.35 -0.1 3,139,890 - BT2515 0.51 -1.3 3,139,900 + BT2515 0.57 -1.3 3,139,900 + BT2515 0.57 -0.1 3,140,032 - -0.8 3,140,092 - -0.1 3,140,103 + BT2516 0.10 -3.1 3,140,103 + BT2516 0.10 -2.1 3,140,125 + BT2516 0.13 -0.8 3,140,126 - BT2516 0.14 -1.7 3,140,126 - BT2516 0.14 -2.9 3,140,214 - BT2516 0.26 -1.0 3,140,217 + BT2516 0.27 -0.1 3,140,230 - BT2516 0.29 -3.5 3,140,265 - BT2516 0.34 +1.5 3,140,312 + BT2516 0.40 +0.9 3,140,325 + BT2516 0.42 -2.6 3,140,412 + BT2516 0.55 -1.7 3,140,415 - BT2516 0.55 +3.4 3,140,418 - BT2516 0.56 -4.2 3,140,430 - BT2516 0.57 +0.9
Or see this region's nucleotide sequence