Experiment: Varel_Bryant_medium_Glucose with Trimethoprim 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2486 and BT2487 are separated by 217 nucleotides BT2487 and BT2488 overlap by 38 nucleotides BT2488 and BT2489 are separated by 301 nucleotides
BT2486: BT2486 - hypothetical protein (NCBI ptt file), at 3,108,332 to 3,111,382
BT2486
BT2487: BT2487 - hypothetical protein (NCBI ptt file), at 3,111,600 to 3,111,788
BT2487
BT2488: BT2488 - integrase (NCBI ptt file), at 3,111,751 to 3,112,698
BT2488
BT2489: BT2489 - 50S ribosomal protein L19 (NCBI ptt file), at 3,113,000 to 3,113,353
BT2489
Position (kb)
3111
3112
3113 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3110.758 kb on + strand, within BT2486 at 3110.759 kb on - strand, within BT2486 at 3110.765 kb on - strand, within BT2486 at 3110.784 kb on - strand, within BT2486 at 3110.808 kb on + strand, within BT2486 at 3110.814 kb on + strand, within BT2486 at 3110.866 kb on + strand, within BT2486 at 3110.867 kb on - strand, within BT2486 at 3110.868 kb on + strand, within BT2486 at 3110.868 kb on + strand, within BT2486 at 3110.871 kb on + strand, within BT2486 at 3110.871 kb on + strand, within BT2486 at 3110.872 kb on - strand, within BT2486 at 3110.982 kb on + strand, within BT2486 at 3110.982 kb on + strand, within BT2486 at 3110.986 kb on + strand, within BT2486 at 3111.070 kb on + strand, within BT2486 at 3111.116 kb on - strand at 3111.148 kb on + strand at 3111.282 kb on - strand at 3111.310 kb on + strand at 3111.447 kb on + strand at 3111.447 kb on + strand at 3111.454 kb on + strand at 3111.475 kb on + strand at 3111.504 kb on + strand at 3111.518 kb on - strand at 3111.547 kb on + strand at 3111.587 kb on - strand at 3111.653 kb on - strand, within BT2487 at 3111.753 kb on - strand at 3111.766 kb on - strand at 3111.848 kb on + strand, within BT2488 at 3111.867 kb on + strand, within BT2488 at 3111.867 kb on + strand, within BT2488 at 3111.868 kb on - strand, within BT2488 at 3111.918 kb on - strand, within BT2488 at 3111.918 kb on - strand, within BT2488 at 3111.918 kb on - strand, within BT2488 at 3111.921 kb on - strand, within BT2488 at 3111.951 kb on + strand, within BT2488 at 3111.952 kb on - strand, within BT2488 at 3111.970 kb on - strand, within BT2488 at 3112.008 kb on - strand, within BT2488 at 3112.008 kb on - strand, within BT2488 at 3112.041 kb on + strand, within BT2488 at 3112.041 kb on + strand, within BT2488 at 3112.042 kb on - strand, within BT2488 at 3112.042 kb on - strand, within BT2488 at 3112.044 kb on - strand, within BT2488 at 3112.044 kb on - strand, within BT2488 at 3112.044 kb on - strand, within BT2488 at 3112.044 kb on - strand, within BT2488 at 3112.113 kb on + strand, within BT2488 at 3112.114 kb on - strand, within BT2488 at 3112.187 kb on + strand, within BT2488 at 3112.191 kb on + strand, within BT2488 at 3112.192 kb on - strand, within BT2488 at 3112.208 kb on - strand, within BT2488 at 3112.209 kb on + strand, within BT2488 at 3112.210 kb on - strand, within BT2488 at 3112.210 kb on - strand, within BT2488 at 3112.210 kb on - strand, within BT2488 at 3112.267 kb on + strand, within BT2488 at 3112.270 kb on + strand, within BT2488 at 3112.270 kb on + strand, within BT2488 at 3112.338 kb on + strand, within BT2488 at 3112.338 kb on + strand, within BT2488 at 3112.421 kb on - strand, within BT2488 at 3112.421 kb on - strand, within BT2488 at 3112.424 kb on + strand, within BT2488 at 3112.425 kb on - strand, within BT2488 at 3112.479 kb on + strand, within BT2488 at 3112.479 kb on + strand, within BT2488 at 3112.480 kb on - strand, within BT2488 at 3112.480 kb on - strand, within BT2488 at 3112.486 kb on - strand, within BT2488 at 3112.504 kb on - strand, within BT2488 at 3112.523 kb on + strand, within BT2488 at 3112.523 kb on + strand, within BT2488 at 3112.612 kb on - strand at 3112.620 kb on - strand at 3112.687 kb on + strand at 3112.796 kb on + strand at 3112.803 kb on - strand at 3113.466 kb on - strand at 3113.479 kb on + strand at 3113.480 kb on - strand at 3113.486 kb on - strand at 3113.487 kb on + strand at 3113.487 kb on + strand at 3113.488 kb on - strand at 3113.488 kb on - strand at 3113.493 kb on + strand at 3113.498 kb on + strand at 3113.518 kb on + strand at 3113.518 kb on + strand at 3113.518 kb on + strand at 3113.519 kb on - strand at 3113.553 kb on + strand at 3113.555 kb on + strand at 3113.612 kb on + strand at 3113.656 kb on - strand at 3113.687 kb on + strand at 3113.687 kb on + strand at 3113.688 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Trimethoprim 0.025 mM remove 3,110,758 + BT2486 0.80 +0.9 3,110,759 - BT2486 0.80 -1.1 3,110,765 - BT2486 0.80 -0.6 3,110,784 - BT2486 0.80 -3.1 3,110,808 + BT2486 0.81 +2.7 3,110,814 + BT2486 0.81 +1.7 3,110,866 + BT2486 0.83 -1.8 3,110,867 - BT2486 0.83 -3.2 3,110,868 + BT2486 0.83 -1.6 3,110,868 + BT2486 0.83 -0.4 3,110,871 + BT2486 0.83 -1.1 3,110,871 + BT2486 0.83 -3.2 3,110,872 - BT2486 0.83 -0.9 3,110,982 + BT2486 0.87 -1.5 3,110,982 + BT2486 0.87 -1.9 3,110,986 + BT2486 0.87 -3.1 3,111,070 + BT2486 0.90 -1.1 3,111,116 - -4.1 3,111,148 + +1.5 3,111,282 - +1.3 3,111,310 + +0.9 3,111,447 + -1.7 3,111,447 + -1.0 3,111,454 + +1.5 3,111,475 + -0.1 3,111,504 + -3.3 3,111,518 - -2.1 3,111,547 + +1.1 3,111,587 - +0.7 3,111,653 - BT2487 0.28 -1.0 3,111,753 - -1.1 3,111,766 - -2.0 3,111,848 + BT2488 0.10 -1.8 3,111,867 + BT2488 0.12 +0.9 3,111,867 + BT2488 0.12 +2.5 3,111,868 - BT2488 0.12 -1.1 3,111,918 - BT2488 0.18 -0.1 3,111,918 - BT2488 0.18 +1.5 3,111,918 - BT2488 0.18 -1.0 3,111,921 - BT2488 0.18 +0.9 3,111,951 + BT2488 0.21 -2.4 3,111,952 - BT2488 0.21 -3.0 3,111,970 - BT2488 0.23 -0.4 3,112,008 - BT2488 0.27 +2.7 3,112,008 - BT2488 0.27 +2.9 3,112,041 + BT2488 0.31 -1.7 3,112,041 + BT2488 0.31 -0.4 3,112,042 - BT2488 0.31 +2.3 3,112,042 - BT2488 0.31 +1.5 3,112,044 - BT2488 0.31 -1.0 3,112,044 - BT2488 0.31 +1.7 3,112,044 - BT2488 0.31 -0.1 3,112,044 - BT2488 0.31 -0.3 3,112,113 + BT2488 0.38 -0.1 3,112,114 - BT2488 0.38 -1.1 3,112,187 + BT2488 0.46 -0.1 3,112,191 + BT2488 0.46 -2.0 3,112,192 - BT2488 0.47 -1.8 3,112,208 - BT2488 0.48 +0.5 3,112,209 + BT2488 0.48 -0.6 3,112,210 - BT2488 0.48 +3.1 3,112,210 - BT2488 0.48 -3.5 3,112,210 - BT2488 0.48 -0.2 3,112,267 + BT2488 0.54 +0.5 3,112,270 + BT2488 0.55 +3.4 3,112,270 + BT2488 0.55 +0.5 3,112,338 + BT2488 0.62 -1.2 3,112,338 + BT2488 0.62 -3.2 3,112,421 - BT2488 0.71 -0.1 3,112,421 - BT2488 0.71 -1.2 3,112,424 + BT2488 0.71 -1.6 3,112,425 - BT2488 0.71 -0.1 3,112,479 + BT2488 0.77 -0.1 3,112,479 + BT2488 0.77 -4.4 3,112,480 - BT2488 0.77 +1.0 3,112,480 - BT2488 0.77 +0.9 3,112,486 - BT2488 0.78 -0.1 3,112,504 - BT2488 0.79 -1.3 3,112,523 + BT2488 0.81 +0.5 3,112,523 + BT2488 0.81 +1.2 3,112,612 - -2.1 3,112,620 - -2.1 3,112,687 + -0.2 3,112,796 + -1.1 3,112,803 - -0.9 3,113,466 - -0.1 3,113,479 + -1.6 3,113,480 - -0.8 3,113,486 - +0.3 3,113,487 + -1.0 3,113,487 + -0.1 3,113,488 - -3.5 3,113,488 - -2.5 3,113,493 + +1.4 3,113,498 + -1.3 3,113,518 + -0.3 3,113,518 + -3.2 3,113,518 + -2.6 3,113,519 - -0.4 3,113,553 + -0.9 3,113,555 + -0.1 3,113,612 + -2.4 3,113,656 - -0.1 3,113,687 + -0.3 3,113,687 + -2.5 3,113,688 - -0.1
Or see this region's nucleotide sequence