Experiment: Varel_Bryant_medium_Glucose with Trimethoprim 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2021 and BT2022 are separated by 227 nucleotides BT2022 and BT2023 overlap by 28 nucleotides BT2023 and BT2024 are separated by 568 nucleotides
BT2021: BT2021 - conserved hypothetical protein, putative non-specific DNA-binding protein (NCBI ptt file), at 2,537,883 to 2,538,410
BT2021
BT2022: BT2022 - hypothetical protein (NCBI ptt file), at 2,538,638 to 2,539,081
BT2022
BT2023: BT2023 - two-component system sensor histidine kinase (NCBI ptt file), at 2,539,054 to 2,541,216
BT2023
BT2024: BT2024 - conserved hypothetical protein (NCBI ptt file), at 2,541,785 to 2,542,450
BT2024
Position (kb)
2539
2540
2541
2542 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2538.193 kb on - strand, within BT2021 at 2538.347 kb on - strand, within BT2021 at 2538.349 kb on + strand, within BT2021 at 2538.350 kb on - strand, within BT2021 at 2538.388 kb on + strand at 2538.389 kb on - strand at 2538.389 kb on - strand at 2538.411 kb on - strand at 2538.411 kb on - strand at 2538.483 kb on + strand at 2538.508 kb on - strand at 2538.516 kb on + strand at 2538.516 kb on + strand at 2538.563 kb on + strand at 2538.564 kb on - strand at 2538.564 kb on - strand at 2538.585 kb on - strand at 2538.655 kb on - strand at 2538.656 kb on + strand at 2538.750 kb on + strand, within BT2022 at 2538.778 kb on - strand, within BT2022 at 2538.910 kb on + strand, within BT2022 at 2539.277 kb on + strand, within BT2023 at 2539.277 kb on + strand, within BT2023 at 2539.277 kb on + strand, within BT2023 at 2539.572 kb on + strand, within BT2023 at 2539.615 kb on - strand, within BT2023 at 2540.266 kb on + strand, within BT2023 at 2540.430 kb on - strand, within BT2023 at 2540.544 kb on + strand, within BT2023 at 2540.547 kb on - strand, within BT2023 at 2540.609 kb on + strand, within BT2023 at 2540.610 kb on - strand, within BT2023 at 2540.654 kb on + strand, within BT2023 at 2540.737 kb on + strand, within BT2023 at 2540.742 kb on - strand, within BT2023 at 2540.750 kb on + strand, within BT2023 at 2540.751 kb on - strand, within BT2023 at 2540.751 kb on - strand, within BT2023 at 2540.854 kb on + strand, within BT2023 at 2541.016 kb on + strand at 2541.016 kb on + strand at 2541.017 kb on - strand at 2541.221 kb on - strand at 2541.260 kb on - strand at 2541.351 kb on - strand at 2541.355 kb on + strand at 2541.358 kb on - strand at 2541.455 kb on + strand at 2541.494 kb on + strand at 2541.578 kb on + strand at 2541.585 kb on + strand at 2541.653 kb on + strand at 2541.653 kb on + strand at 2541.654 kb on - strand at 2541.654 kb on - strand at 2541.806 kb on - strand at 2541.878 kb on + strand, within BT2024 at 2541.878 kb on + strand, within BT2024 at 2541.909 kb on + strand, within BT2024 at 2541.947 kb on - strand, within BT2024 at 2542.111 kb on - strand, within BT2024 at 2542.201 kb on + strand, within BT2024 at 2542.201 kb on + strand, within BT2024 at 2542.202 kb on - strand, within BT2024
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Trimethoprim 0.025 mM remove 2,538,193 - BT2021 0.59 -0.9 2,538,347 - BT2021 0.88 -0.9 2,538,349 + BT2021 0.88 -0.3 2,538,350 - BT2021 0.88 -1.3 2,538,388 + -0.7 2,538,389 - -1.9 2,538,389 - -1.3 2,538,411 - -2.5 2,538,411 - -0.6 2,538,483 + -0.7 2,538,508 - -1.9 2,538,516 + -2.4 2,538,516 + -0.9 2,538,563 + -0.9 2,538,564 - +0.6 2,538,564 - -3.2 2,538,585 - +0.1 2,538,655 - +0.3 2,538,656 + -3.2 2,538,750 + BT2022 0.25 +3.9 2,538,778 - BT2022 0.32 -0.9 2,538,910 + BT2022 0.61 -1.4 2,539,277 + BT2023 0.10 -0.9 2,539,277 + BT2023 0.10 -1.9 2,539,277 + BT2023 0.10 +1.7 2,539,572 + BT2023 0.24 +1.3 2,539,615 - BT2023 0.26 -3.8 2,540,266 + BT2023 0.56 +1.3 2,540,430 - BT2023 0.64 +2.0 2,540,544 + BT2023 0.69 -2.2 2,540,547 - BT2023 0.69 -0.4 2,540,609 + BT2023 0.72 +2.3 2,540,610 - BT2023 0.72 +1.7 2,540,654 + BT2023 0.74 +1.1 2,540,737 + BT2023 0.78 -0.9 2,540,742 - BT2023 0.78 -1.5 2,540,750 + BT2023 0.78 -1.3 2,540,751 - BT2023 0.78 +2.9 2,540,751 - BT2023 0.78 +0.6 2,540,854 + BT2023 0.83 -3.6 2,541,016 + +1.3 2,541,016 + -0.3 2,541,017 - -1.5 2,541,221 - -2.9 2,541,260 - -1.5 2,541,351 - -3.1 2,541,355 + +1.1 2,541,358 - -0.7 2,541,455 + +0.1 2,541,494 + +2.4 2,541,578 + +0.8 2,541,585 + +0.1 2,541,653 + -1.9 2,541,653 + -0.9 2,541,654 - -0.9 2,541,654 - +2.4 2,541,806 - -2.5 2,541,878 + BT2024 0.14 +3.3 2,541,878 + BT2024 0.14 -0.5 2,541,909 + BT2024 0.19 -2.5 2,541,947 - BT2024 0.24 -4.6 2,542,111 - BT2024 0.49 -3.8 2,542,201 + BT2024 0.62 -3.6 2,542,201 + BT2024 0.62 +0.2 2,542,202 - BT2024 0.63 -2.2
Or see this region's nucleotide sequence