Experiment: Varel_Bryant_medium_Glucose with Trimethoprim 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1539 and BT1540 are separated by 3 nucleotides BT1540 and BT1541 are separated by 88 nucleotides BT1541 and BT1542 are separated by 103 nucleotides
BT1539: BT1539 - cytidine deaminase (NCBI ptt file), at 1,899,104 to 1,899,499
BT1539
BT1540: BT1540 - transcriptional regulator (NCBI ptt file), at 1,899,503 to 1,900,342
BT1540
BT1541: BT1541 - conserved hypothetical protein (NCBI ptt file), at 1,900,431 to 1,901,012
BT1541
BT1542: BT1542 - putative pyridine nucleotide-disulfide oxidoreductase (NCBI ptt file), at 1,901,116 to 1,902,489
BT1542
Position (kb)
1900
1901
1902 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1899.431 kb on - strand, within BT1539 at 1899.435 kb on - strand, within BT1539 at 1899.596 kb on + strand, within BT1540 at 1899.604 kb on + strand, within BT1540 at 1899.657 kb on + strand, within BT1540 at 1899.657 kb on + strand, within BT1540 at 1899.729 kb on - strand, within BT1540 at 1900.037 kb on + strand, within BT1540 at 1900.047 kb on + strand, within BT1540 at 1900.086 kb on - strand, within BT1540 at 1900.141 kb on + strand, within BT1540 at 1900.216 kb on + strand, within BT1540 at 1900.308 kb on + strand at 1900.343 kb on + strand at 1900.496 kb on + strand, within BT1541 at 1900.513 kb on + strand, within BT1541 at 1900.704 kb on + strand, within BT1541 at 1900.723 kb on + strand, within BT1541 at 1900.793 kb on - strand, within BT1541 at 1900.827 kb on - strand, within BT1541 at 1900.827 kb on - strand, within BT1541 at 1901.062 kb on + strand at 1901.063 kb on - strand at 1901.063 kb on - strand at 1901.084 kb on - strand at 1901.125 kb on + strand at 1901.125 kb on + strand at 1901.125 kb on + strand at 1901.136 kb on + strand at 1901.139 kb on + strand at 1901.252 kb on + strand at 1901.260 kb on - strand, within BT1542 at 1901.308 kb on + strand, within BT1542 at 1901.326 kb on - strand, within BT1542 at 1901.334 kb on + strand, within BT1542 at 1901.335 kb on - strand, within BT1542 at 1901.341 kb on + strand, within BT1542 at 1901.342 kb on - strand, within BT1542 at 1901.342 kb on - strand, within BT1542 at 1901.434 kb on + strand, within BT1542 at 1901.435 kb on - strand, within BT1542 at 1901.443 kb on - strand, within BT1542 at 1901.556 kb on + strand, within BT1542 at 1901.556 kb on + strand, within BT1542 at 1901.558 kb on + strand, within BT1542 at 1901.558 kb on + strand, within BT1542 at 1901.559 kb on - strand, within BT1542 at 1901.588 kb on - strand, within BT1542 at 1901.595 kb on + strand, within BT1542 at 1901.638 kb on - strand, within BT1542 at 1901.681 kb on - strand, within BT1542 at 1901.884 kb on + strand, within BT1542 at 1901.884 kb on + strand, within BT1542 at 1902.009 kb on - strand, within BT1542 at 1902.012 kb on + strand, within BT1542
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Trimethoprim 0.025 mM remove 1,899,431 - BT1539 0.83 -1.3 1,899,435 - BT1539 0.84 -0.9 1,899,596 + BT1540 0.11 -0.7 1,899,604 + BT1540 0.12 -3.4 1,899,657 + BT1540 0.18 +1.7 1,899,657 + BT1540 0.18 -0.9 1,899,729 - BT1540 0.27 +1.2 1,900,037 + BT1540 0.64 -3.7 1,900,047 + BT1540 0.65 -0.9 1,900,086 - BT1540 0.69 -0.9 1,900,141 + BT1540 0.76 -1.5 1,900,216 + BT1540 0.85 +0.1 1,900,308 + +0.3 1,900,343 + -1.6 1,900,496 + BT1541 0.11 -0.9 1,900,513 + BT1541 0.14 -3.2 1,900,704 + BT1541 0.47 -0.3 1,900,723 + BT1541 0.50 +1.0 1,900,793 - BT1541 0.62 -0.2 1,900,827 - BT1541 0.68 +0.1 1,900,827 - BT1541 0.68 +1.1 1,901,062 + -0.9 1,901,063 - +3.1 1,901,063 - +0.7 1,901,084 - -2.7 1,901,125 + -1.5 1,901,125 + +0.1 1,901,125 + -3.7 1,901,136 + -1.5 1,901,139 + -3.4 1,901,252 + -1.2 1,901,260 - BT1542 0.10 -1.9 1,901,308 + BT1542 0.14 -2.9 1,901,326 - BT1542 0.15 -1.1 1,901,334 + BT1542 0.16 -3.9 1,901,335 - BT1542 0.16 -1.7 1,901,341 + BT1542 0.16 -0.9 1,901,342 - BT1542 0.16 +2.9 1,901,342 - BT1542 0.16 -3.8 1,901,434 + BT1542 0.23 -3.5 1,901,435 - BT1542 0.23 +0.1 1,901,443 - BT1542 0.24 +1.1 1,901,556 + BT1542 0.32 -0.9 1,901,556 + BT1542 0.32 +0.1 1,901,558 + BT1542 0.32 -1.5 1,901,558 + BT1542 0.32 -2.5 1,901,559 - BT1542 0.32 -2.9 1,901,588 - BT1542 0.34 -0.9 1,901,595 + BT1542 0.35 +0.1 1,901,638 - BT1542 0.38 -0.8 1,901,681 - BT1542 0.41 -3.1 1,901,884 + BT1542 0.56 -2.2 1,901,884 + BT1542 0.56 -2.6 1,902,009 - BT1542 0.65 +0.1 1,902,012 + BT1542 0.65 +1.1
Or see this region's nucleotide sequence