Experiment: Varel_Bryant_medium_Glucose with Trimethoprim 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0706 and BT0707 are separated by 145 nucleotides BT0707 and BT0708 are separated by 299 nucleotides BT0708 and BT0709 are separated by 224 nucleotides
BT0706: BT0706 - putative N-acetylmuramoyl-L-alanine amidase (NCBI ptt file), at 879,984 to 880,367
BT0706
BT0707: BT0707 - conserved hypothetical protein (NCBI ptt file), at 880,513 to 881,001
BT0707
BT0708: BT0708 - hypothetical protein (NCBI ptt file), at 881,301 to 881,546
BT0708
BT0709: BT0709 - hypothetical protein (NCBI ptt file), at 881,771 to 884,146
BT0709
Position (kb) 881
882 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 880.324 kb on + strand, within BT0706 at 880.370 kb on + strand at 880.448 kb on + strand at 880.463 kb on + strand at 880.514 kb on + strand at 880.666 kb on + strand, within BT0707 at 880.710 kb on + strand, within BT0707 at 880.844 kb on + strand, within BT0707 at 881.147 kb on + strand at 881.147 kb on + strand at 881.452 kb on - strand, within BT0708 at 881.460 kb on - strand, within BT0708 at 881.463 kb on + strand, within BT0708 at 881.463 kb on + strand, within BT0708 at 881.598 kb on + strand at 881.618 kb on + strand at 881.618 kb on + strand at 881.622 kb on - strand at 881.770 kb on - strand at 881.995 kb on + strand at 881.996 kb on - strand at 882.010 kb on + strand, within BT0709 at 882.051 kb on - strand, within BT0709 at 882.182 kb on - strand, within BT0709
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Trimethoprim 0.025 mM remove 880,324 + BT0706 0.89 -1.1 880,370 + -1.8 880,448 + -2.1 880,463 + -2.4 880,514 + -0.1 880,666 + BT0707 0.31 +2.1 880,710 + BT0707 0.40 +0.4 880,844 + BT0707 0.68 +0.5 881,147 + -1.3 881,147 + -1.1 881,452 - BT0708 0.61 -0.1 881,460 - BT0708 0.65 -2.2 881,463 + BT0708 0.66 -0.1 881,463 + BT0708 0.66 -3.9 881,598 + -3.8 881,618 + -1.6 881,618 + -0.4 881,622 - -1.6 881,770 - -1.1 881,995 + +0.9 881,996 - -2.6 882,010 + BT0709 0.10 +0.9 882,051 - BT0709 0.12 +1.3 882,182 - BT0709 0.17 -0.6
Or see this region's nucleotide sequence