Experiment: Varel_Bryant_medium_Glucose with Trimethoprim 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0221 and BT0222 are separated by 18 nucleotides BT0222 and BT0223 are separated by 70 nucleotides BT0223 and BT0224 are separated by 128 nucleotides
BT0221: BT0221 - conserved hypothetical protein (NCBI ptt file), at 241,657 to 242,916
BT0221
BT0222: BT0222 - putative two-component system sensor kinase, but no kinase domain (NCBI ptt file), at 242,935 to 243,960
BT0222
BT0223: BT0223 - two-component system response regulator (NCBI ptt file), at 244,031 to 244,723
BT0223
BT0224: BT0224 - hypothetical protein (NCBI ptt file), at 244,852 to 245,256
BT0224
Position (kb) 242
243
244 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 241.997 kb on + strand, within BT0221 at 241.998 kb on - strand, within BT0221 at 241.998 kb on - strand, within BT0221 at 241.998 kb on - strand, within BT0221 at 241.998 kb on - strand, within BT0221 at 242.078 kb on + strand, within BT0221 at 242.119 kb on - strand, within BT0221 at 242.157 kb on + strand, within BT0221 at 242.231 kb on - strand, within BT0221 at 242.256 kb on - strand, within BT0221 at 242.266 kb on - strand, within BT0221 at 242.277 kb on + strand, within BT0221 at 242.309 kb on + strand, within BT0221 at 242.310 kb on - strand, within BT0221 at 242.358 kb on + strand, within BT0221 at 242.458 kb on + strand, within BT0221 at 242.500 kb on + strand at 242.501 kb on - strand, within BT0221 at 242.537 kb on + strand, within BT0221 at 242.538 kb on - strand, within BT0221 at 242.542 kb on + strand, within BT0221 at 242.584 kb on + strand, within BT0221 at 242.610 kb on + strand, within BT0221 at 242.621 kb on + strand, within BT0221 at 242.622 kb on - strand, within BT0221 at 242.658 kb on + strand, within BT0221 at 242.659 kb on - strand, within BT0221 at 242.745 kb on + strand, within BT0221 at 242.780 kb on + strand, within BT0221 at 242.851 kb on + strand at 242.852 kb on - strand at 242.940 kb on + strand at 242.982 kb on - strand at 242.998 kb on + strand at 243.004 kb on - strand at 243.021 kb on - strand at 243.262 kb on - strand, within BT0222 at 243.267 kb on + strand, within BT0222 at 243.280 kb on - strand, within BT0222 at 243.282 kb on - strand, within BT0222 at 243.408 kb on + strand, within BT0222 at 243.409 kb on - strand, within BT0222 at 243.520 kb on + strand, within BT0222 at 243.553 kb on + strand, within BT0222 at 243.555 kb on + strand, within BT0222 at 243.558 kb on - strand, within BT0222 at 243.672 kb on + strand, within BT0222 at 243.673 kb on - strand, within BT0222 at 243.697 kb on - strand, within BT0222 at 243.697 kb on - strand, within BT0222 at 243.721 kb on - strand, within BT0222 at 243.800 kb on + strand, within BT0222 at 243.802 kb on + strand, within BT0222 at 243.802 kb on + strand, within BT0222 at 243.803 kb on - strand, within BT0222 at 243.803 kb on - strand, within BT0222 at 243.830 kb on + strand, within BT0222 at 243.831 kb on - strand, within BT0222 at 244.050 kb on + strand at 244.051 kb on - strand at 244.115 kb on + strand, within BT0223 at 244.116 kb on - strand, within BT0223 at 244.344 kb on + strand, within BT0223 at 244.446 kb on + strand, within BT0223 at 244.580 kb on + strand, within BT0223 at 244.594 kb on + strand, within BT0223 at 244.741 kb on + strand at 244.776 kb on + strand at 244.777 kb on - strand at 244.779 kb on - strand at 244.779 kb on - strand at 244.833 kb on + strand at 244.841 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Trimethoprim 0.025 mM remove 241,997 + BT0221 0.27 -1.7 241,998 - BT0221 0.27 -0.1 241,998 - BT0221 0.27 +0.7 241,998 - BT0221 0.27 -1.1 241,998 - BT0221 0.27 -2.1 242,078 + BT0221 0.33 -0.1 242,119 - BT0221 0.37 -0.1 242,157 + BT0221 0.40 -1.7 242,231 - BT0221 0.46 +1.5 242,256 - BT0221 0.48 -1.7 242,266 - BT0221 0.48 +0.2 242,277 + BT0221 0.49 -0.1 242,309 + BT0221 0.52 -0.1 242,310 - BT0221 0.52 -1.1 242,358 + BT0221 0.56 +1.5 242,458 + BT0221 0.64 -1.1 242,500 + -1.1 242,501 - BT0221 0.67 -1.1 242,537 + BT0221 0.70 +1.9 242,538 - BT0221 0.70 -0.1 242,542 + BT0221 0.70 -1.5 242,584 + BT0221 0.74 -1.7 242,610 + BT0221 0.76 -0.1 242,621 + BT0221 0.77 -2.9 242,622 - BT0221 0.77 -2.4 242,658 + BT0221 0.79 +2.5 242,659 - BT0221 0.80 -0.4 242,745 + BT0221 0.86 -2.7 242,780 + BT0221 0.89 -2.4 242,851 + -1.9 242,852 - -1.1 242,940 + -4.3 242,982 - +2.3 242,998 + -1.9 243,004 - -2.1 243,021 - -2.6 243,262 - BT0222 0.32 -2.6 243,267 + BT0222 0.32 -2.5 243,280 - BT0222 0.34 -2.6 243,282 - BT0222 0.34 +2.3 243,408 + BT0222 0.46 -1.1 243,409 - BT0222 0.46 +0.9 243,520 + BT0222 0.57 +3.1 243,553 + BT0222 0.60 -2.6 243,555 + BT0222 0.60 -0.5 243,558 - BT0222 0.61 -0.1 243,672 + BT0222 0.72 +0.7 243,673 - BT0222 0.72 -1.6 243,697 - BT0222 0.74 -0.1 243,697 - BT0222 0.74 -1.9 243,721 - BT0222 0.77 -1.6 243,800 + BT0222 0.84 -0.1 243,802 + BT0222 0.85 -3.9 243,802 + BT0222 0.85 -1.0 243,803 - BT0222 0.85 -1.3 243,803 - BT0222 0.85 -1.8 243,830 + BT0222 0.87 -1.1 243,831 - BT0222 0.87 +1.5 244,050 + -1.7 244,051 - -1.7 244,115 + BT0223 0.12 -2.2 244,116 - BT0223 0.12 -0.1 244,344 + BT0223 0.45 +0.2 244,446 + BT0223 0.60 -2.1 244,580 + BT0223 0.79 -1.1 244,594 + BT0223 0.81 -1.1 244,741 + +2.2 244,776 + -3.4 244,777 - -2.4 244,779 - -3.5 244,779 - +3.1 244,833 + +2.5 244,841 - -1.7
Or see this region's nucleotide sequence